A5024732143- Blood properties and coagulation
- Optical Imaging and Spectroscopy Techniques
- Erythrocyte Function and Pathophysiology
- Photoacoustic and Ultrasonic Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Thermoregulation and physiological responses
- Optical Coherence Tomography Applications
- Microfluidic and Bio-sensing Technologies
- Cardiovascular Health and Disease Prevention
- Skin Protection and Aging
- Laser Design and Applications
- Laser Applications in Dentistry and Medicine
- Optical Polarization and Ellipsometry
- Digital Imaging for Blood Diseases
- Chemical and Physical Studies
- Peripheral Artery Disease Management
- Ocular and Laser Science Research
- Protein Interaction Studies and Fluorescence Analysis
- Non-Invasive Vital Sign Monitoring
- Atmospheric aerosols and clouds
- Slime Mold and Myxomycetes Research
- Orbital Angular Momentum in Optics
- Advanced Fluorescence Microscopy Techniques
- Fluid Dynamics and Turbulent Flows
- Infrared Thermography in Medicine
Lomonosov Moscow State University
2015-2024
Moscow State University
2008-2021
Laboratoire Interdisciplinaire de Physique
2020
Centre National de la Recherche Scientifique
2020
International University in Moscow
2009-2019
International Laser Center
2011-2012
P.N. Lebedev Physical Institute of the Russian Academy of Sciences
2011
University of Oulu
2007-2008
Pirogov Russian National Research Medical University
1994
Institute of Biophysics
1984
Protecting human skin against harmful UV-B radiation coming from the sun is currently a problem. Due to decreased thickness of ozone layer, more dangerous amount light reaches surface our planet. This causes increased frequency diseases. Titanium dioxide (TiO2) fine particles are embedded with sunscreens into effectively attenuate radiation. study evaluates most appropriate size such assuming they spheres. The distribution TiO2 within skin, achieved topically applied sunscreens, determined...
Nanodiamonds (ND) have emerged to be a widely-discussed nanomaterial for their applications in biological studies and medical diagnostics treatment. The potentials been successfully demonstrated cellular tissue models vitro. For applications, further vivo on various become important. One of the most challenging possibilities ND biomedical application is controllable drug delivery tracing. That usually assumes interaction with blood system. In this work, we study rat analyze how surface...
The papillary dermis of human skin is responsible for its biomechanical properties and supply epidermis with chemicals. Dermis mainly composed structural protein molecules, including collagen elastin, contains blood capillaries. Connective tissue diseases, as well cardiovascular complications have manifestations on the molecular level in (e.g. alteration I III content) capillary structure. In this paper we assessed structure internal external regions capillaries using two-photon fluorescence...
Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation red blood cells (RBCs) were studied at the level cell doublets to assess RBC interaction mechanics. Measurements performed under in vitro conditions plasma fibrinogen + albumin solutions. The kinetics was found exhibit different behavior depending on environment. In contrast, similar all solutions qualitatively quantitatively, demonstrating a significant contribution proteins process. impact study assessing...
Recently there has been a strong demand to protect human skin against negative effects of the UV solar light. This problem is interesting due increased frequency diseases caused by such radiation. We aim evaluate how optical properties horny layer can be effectively changed imbedding TiO2 fine particles achieve maximal attenuation In-depth distribution embedded into multiple administration sunscreens determined experimentally using tape-stripping technique. A computer code implementing Monte...
Blood cell analysis is one of the standard clinical tests.Despite widespread use exogenous markers for blood quantification, label-free optical methods are still high demand due to their possibility in vivo application and signal specific biochemical state provided by native fluorophores.Here we report results characterization using fluorescence imaging techniques flow-cytometry.Autofluorescence parameters different types -white cells, red erythrophagocytic cells -are assessed analyzed terms...
The red blood cells (RBC) aggregation is of current basic science and clinical interest, as a determinant microcirculation. Thus, the measurement assessment RBC property (aggregability) state at different physiologic conditions human individual or laboratory animal are an important issue. In this paper, in order to assess dynamics interaction, optical tweezers were used probe forces during doublet formation disruption. We show that autologous plasma, aggregating disaggregating have absolute...
Endogenous autofluorescence of biological tissues is an important source information for biomedical diagnostics. Despite the molecular complexity tissues, list commonly known fluorophores strictly limited. Still, question sources red and near-infrared excited remains open. In this work we demonstrated that oxidation products organic components (lipids, proteins, amino acids, etc.) can serve as such autofluorescence. Using model solutions cell systems (human keratinocytes) under oxidative...
The <i>Journal of Biomedical Optics</i> (JBO) is a Gold Open Access journal that publishes peer-reviewed papers on the use novel optical systems and techniques for improved health care biomedical research.
Ever increasing use of engineered nanodiamond (ND) into the human blood for various biomedical studies and applications has increased demand to thoroughly understand interaction NDs with its effect on coagulation. Here, we report study adsorption plasma sized carboxylated nanodiamonds (cNDs) using UV/visible spectroscopy Fourier transform infrared (FTIR). The 5 nm 100 cNDs is confirmed from spectra. FTIR shows minor change in shape amide I absorption peak (1600–1700 cm− 1) indicating that...
We present the results of a native fluorescence spectroscopy study blood plasma rats with experimental diabetes. It was shown that emission band shape at 320 nm excitation is most indicative hyperglycemia in samples. provide interpretation this fact based on changes reduced nicotinamide adenine dinucleotide phosphate concentration due to glucose-related metabolic pathways and protein fluorescent cross-linking formation following nonenzymatic glycation.
Red blood cell (RBC) aggregation highly influences hemorheology and microcirculation in the human body. The properties of RBCs can vary due to numerous factors, including RBC age. aim this work was estimate vitro differences different age populations single-cell experiments using laser tweezers. from five healthy volunteers were separated into four subpopulations by Percoll density gradient centrifugation. Each subpopulation separately resuspended autologous plasma or dextran 70 kDa (50...
Thioflavin T (ThT) assay is extensively used for studying fibrillation kinetics in vitro. However, the differences time course of ThT fluorescence intensity and lifetime other physical parameters system, such as particle size distribution, raise questions about correct interpretation aggregation kinetics. In this work, we focused on investigation mechanisms, which underlay difference sensitivity to formation protein aggregates during by example insulin binding globular proteins. The...
Titanium dioxide (TiO2) is considered to be a nontoxic material and widely used in number of everyday products, such as sunscreen. TiO2 nanoparticles (NP) are also prospective agents for photodynamic therapy drug delivery. These applications require an understanding the potential effects on blood system its components upon administration. In presented work, we analyze interaction different crystal phases (anatase rutile) with individual rat Red Blood Cells (RBC) influence oxygenation state...
We have fabricated double-beam optical tweezers and demonstrated the possibility of their use for measuring interaction forces between red blood cells (erythrocytes). It has been established experimentally that prolonged trapping in a tightly focused laser beam does not cause any visible changes shape or size. measured aggregate, deformed by tweezers.
An elevated concentration of fibrinogen in blood is a significant risk factor during many pathological diseases, as it leads to an increase red cells (RBC) aggregation, resulting hemorheological disorders. Despite the biomedical importance, mechanisms fibrinogen-induced RBC aggregation are still debatable. One discussed models non-specific adsorption macromolecules onto membrane, leading bridging aggregates. However, recent works point specific character interaction between and membrane....
We present here a novel phantom for optical coherence tomography (OCT) made of polyvinyl chloride-plastisol (PVCP). The properties PVCP were estimated by the Mie theory and deduced from OCT measurements. Titanium dioxide (TiO<sub>2</sub>) powder black plastic colour (light-absorbing ink) used to introduce scattering create capillary structure, respectively.
Adsorption of such blood plasma proteins as albumin and g-globulin on diamond nanoparticles size around 5 nm 100 is observed studied using laser-optical methods. The adsorption at physiological pH 7.4 found weaker than that enzyme protein lysozyme. variations in the Fourier Transform Infrared (FTIR) spectra may be due to structural transformations adsorbed protein. Using lysozyme a test we show leading observable changes FTIR spectrum (the band Amide I) also induces significant decrease...
We report about manufacturing of fully functional capillary network embedded into the multilayer tissue phantom. Polyvinyl chloride-plastisol was used as a host transparent medium. Scattering introduced by adding TiO<sub>2</sub>submicron particles. OCT technique to characterize manufactured phantoms and monitor vessels filling with different liquids.
Red blood cell (RBC) aggregation is an intrinsic property of the that has a direct effect on viscosity and circulation. Nevertheless, mechanism behind RBC not been confirmed still under investigation with two major hypotheses, known as “depletion layer” “cross-bridging.” We aim to ultimately understand clarify both models. To measure interaction in vitro different suspensions (including plasma, isotonic solution fibrinogen, fibrinogen albumin, phosphate buffer saline) while moving aggregate...