Blastocystis is a common intestinal pathogen of humans and variety animals, with various host-specific subtypes. The aim this study was to determine the prevalence subtype distribution in domestic Thailand. 113 stool samples were collected from pigs, goats, cattle Ayutthaya Province (AP; central Thailand) 218 dogs, cats, chickens, Kanchanaburi (KP; western Thailand). detected by nested PCR targeting SSU rRNA gene. Subtypes identified DNA sequencing, phylogenetic analysis conducted. overall...

Citrus reticulata Blanco and aurantifolia are the edible plants which contain several biological properties including antibacterial activity. The aims of present study were to determine chemical compositions evaluate activities citrus essential oils extracted from fruit peels C. (CREO) (CAEO), alone in combination with gentamicin, against a panel clinically isolated methicillin-resistant S. aureus (MRSA) (n = 40) methicillin-susceptible (MSSA) 45). Gas chromatography-mass spectrometry...

Blastocystis inhabits the digestive tracts of a diverse range hosts. Transmission patterns, including host specificity, and clinical public health significance in humans remain poorly understood. This study aimed to investigate distribution genetic diversity herbivorous carnivorous reptiles Eastern Thailand. A total 501 faecal samples were collected from 363 iguanas, 79 bearded dragons, 50 tortoises, nine snakes an animal breeding farm Chonburi Province, Detection differentiation was based...

Zoonotic Cryptosporidium spp., particularly C. meleagridis, canis, and felis, are enteric protozoa responsible for major public health concerns around the world. To determine spread of this parasite in Thailand, we conducted molecular identification spp. from animal samples country, by collecting investigating feces seagulls (Chroicocephalus brunnicephalus Chroicocephalus ridibundus), domestic pigeons (Columba livia domestica), dogs, cats. Seagull pigeon were collected at seaside on...

Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of in villagers living on Thai-Myanmar border, where risk parasitic infection high. total 207 stool samples were collected DNA extracted. PCR sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene performed. The 37.2% (77/207). ST3 (19.8%; 41/207) predominant subtype,...

Blastocystis is a unicellular protist most commonly detected in humans and variety of animals. The predominant mode its transmission the fecal–oral route, but zoonotic potential not completely understood. objective this study was to determine presence genetic diversity on pig farms Nakhon Pathom Province, Central Thailand. A total 154 human 90 stool samples were collected analyzed. Nested PCR 35.55% 6.49% samples. Subtyping based regions small-subunit ribosomal RNA (SSU rRNA) gene identified...

Enterocytozoon bieneusi has been increasingly reported to infect domestic animals and humans, with human infections primarily as zoonotic in origin. The aim of the present study was determine presence genotype E. humans central Thailand by testing stool samples 200 apparently healthy 73 goats, 60 cattle 65 pigs using nested-PCR/ sequence analysis based on ITS region SSU rRNA genes.E. tested positive 2 (1%) collected from 56 (28.3%) 198 animals. highest prevalence observed (39/65, 60%),...

Giardia duodenalis is a major pathogen of the gastrointestinal tract, and infections impact both human health economy. The ongoing issues with drug resistance side effects current anti-Giardia treatments highlight urgent need for new therapeutic options. This study focused on investigating in vitro efficacy crude extracts Stemona collinsiae from Thailand against G. duodenalis. S. was initially extracted hexane, followed sequentially by dichloromethane, ethanol, water. were tested growth...

The escalating global incidence of antimicrobial resistance poses a significant public health challenge. In response, exploring alternative agents, particularly derived from plants, becomes crucial to alleviate the selective pressure exerted by conventional antibiotics. aim this study was characterize composition essential oil extracted Litsea cubeba fruits and evaluate its potential, along with major compound, across solid, liquid, vapor phases. activity assessed against diverse range human...

Abstract Background Pentatrichomonas hominis inhabits the digestive tracts of several vertebrates, such as humans, monkeys, pigs, dogs, cats and rats. This protozoan was originally considered a commensal tract but has subsequently been identified potential zoonotic parasite causative agent diarrhoea. Molecular techniques are more sensitive specific to detect P. hominis. study aimed determine presence genetic diversity in animals Thailand. A total 403 faecal samples were collected from 119...

Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of in villagers living along Chao Phraya River, Ayutthaya Province, Thailand, assess risk infection. In total, 220 stool samples were collected, DNA extracted. PCR sequencing performed with primers targeting small-subunit ribosomal RNA (SSU rRNA) genes. present 5.9% (13/220) samples, ST3...

Abstract Background Giardia duodenalis causes giardiasis in humans, particularly developing countries. Despite the availability of treatments, resistance to some commercial anti- drugs has been reported addition their harmful side effects. Therefore, novel treatments for are required. In this study, we aimed assess vitro activity crude extracts Ageratum conyzoides against G. trophozoites. Methods Plants were classified into three groups based on flower colors: white (W), purple (P), and...

Naegleria fowleri is the causative agent of fatal disease primary amebic meningoencephalitis. Detection N. using conventional culture and biochemical-based assays time-consuming laborious, while molecular techniques, such as PCR, require laboratory skills expensive equipment. We developed evaluated a novel loop-mediated isothermal amplification (LAMP) assay targeting virulence-related gene for fowleri. Time to results about 90 min products were easily detected visually hydroxy naphthol blue....

To determine the ability of oysters to trap and maintain viable Cryptosporidium oocysts, feasibility multiplication in oysters' organs. Seventy were raised experimentally seeded natural seawater for up 3 months, with weekly oocysts inoculations. non-viable, as well other stages detected using two immunofluorescence vital staining techniques (Sporo-Glo Merifluor®) confocal microscopy. Viability rate at various times after inoculations calculated. found most concentrated digestive organs than...

Crude extracts and essential oils of A. conyzoides were tested with larva adult stages Ae. aegypti mosquitoes to determine their insecticidal properties. The crude came from three varieties (with white flowers, purple or white-purple flowers) two places on each plant (leaves flowers), giving six types overall: leaf-white (LW); leaf-purple (LP); leaf (LW-P); flower-white (FW); flower-purple (FP); flower (FW-P). Chemical constituents components the identified using gas chromatography-mass...

Giardia duodenalis is a significant protozoan that affects humans and animals. An estimated 280 million G. diarrheal cases are recorded annually. Pharmacological therapy crucial for controlling giardiasis. Metronidazole the first-line treating Several metronidazole targets have been proposed. However, downstream signaling pathways of these with respect to their antigiardial action unclear. In addition, several giardiasis demonstrated treatment failures drug resistance. Therefore, development...

Enterocytozoon bieneusi and Cryptosporidium spp. are prevalent zoonotic parasites associated with a high burden among children. To date only limited molecular epidemiological data on E. in humans living Thailand has been published.PCR-based tools were used to detect characterize The internal transcribed spacer (ITS) region of the rRNA gene was investigate bieneusi, small subunit (SSU) spp., 697 fecal samples from villagers school children rural areas analyzed.The infection rates 2.15%...

Diabetes mellitus (DM) is a major global public health problem with an increasing prevalence. DM increases the risk of infections caused by bacteria, fungi, viruses, and parasites. We examined prevalence, subtypes, factors Blastocystis infection in patients without central Thailand. Stool samples questionnaires were obtained from 130 people group 100 non-DM group. was identified via nested polymerase chain reaction subtyped sequencing partial small-subunit ribosomal RNA (SSU rRNA) gene....

Bovine neosporosis is among the main causes of abortion in cattle worldwide, causing serious economic losses beef and dairy industries. A highly sensitive specific diagnostic method for assessment epidemiology disease, as well it surveillance management, imperative, due to absence an effective treatment or vaccine against neosporosis. In present study, immunodiagnostic performance Neospora caninum peroxiredoxin 2 (NcPrx2), microneme 4 (NcMIC4), surface antigen 1 (NcSAG1) detect IgG...

Even though treatment of symptomatic cases as well improving hygiene and sanitation conditions decrease giardiasis in underdeveloped and/or developing countries, Giardia duodenalis infection is still frequently encountered especially children living crowded leading to public health problems.We studied the prevalence G. 274 asymptomatic Thai boy orphans, in/around Bangkok metropolitan area, by use direct fecal smear formalin-ethyl acetate techniques.This was done explore our hypothesis that...