A5033133780- Bone Metabolism and Diseases
- Bone health and treatments
- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Connective tissue disorders research
- Inflammatory mediators and NSAID effects
Ewha Womans University
2020-2024
Abstract Branched-chain aminotransferase 1 (BCAT1) transfers the amine group on branched-chain amino acids (BCAAs) to alpha-ketoglutarate. This generates glutamate along with alpha-keto that are eventually oxidized provide cell energy. BCAT1 thus plays a critical role in sustaining BCAA concentrations and availability as an energy source. Osteoclasts have high metabolic needs during differentiation. When we assessed levels of bone marrow macrophages (BMMs) were undergoing receptor activator...
Abstract Osteoarthritis (OA) is a progressive and irreversible degenerative joint disease that characterized by cartilage destruction, osteophyte formation, subchondral bone remodeling, synovitis. Despite affecting millions of patients, effective safe disease-modifying osteoarthritis drugs are lacking. Here we reveal an unexpected role for the small molecule 5-aminosalicylic acid (5-ASA), which used as anti-inflammatory drug in ulcerative colitis. We show 5-ASA competes with...
Mature osteoclasts degrade bone matrix by exocytosis of active proteases from secretory lysosomes through a ruffled border. However, the molecular mechanisms underlying lysosomal trafficking and secretion in remain largely unknown. Here, we show with GeneChip analysis that RUN FYVE domain-containing protein 4 (RUFY4) is strongly upregulated during osteoclastogenesis. Mice lacking Rufy4 exhibited high trabecular mass phenotype abnormalities osteoclast function vivo. Furthermore, deleting did...
Abstract Actin rings are unique structures that facilitate the attachment of osteoclasts to bone matrix during resorption. Previous studies have shown tetraspanin7 (TSPAN7) plays an important role in reorganization cytoskeleton necessary for bone‐resorbing activity osteoclasts. However, questions remain as mechanisms by which TSPAN7 regulates this cytoskeletal rearrangement. In study, we investigated roles deleting Tm4sf2 gene mice, encodes TSPAN7. The global knockout model showed protective...
Osteoclast-mediated inflammatory bone resorption is a major cause of many disorders, including rheumatoid arthritis and periodontitis. However, the mechanisms regulating osteoclast differentiation in settings are not well understood. We demonstrate here that early estrogen-induced gene 1 (EEIG1)-deficient mice protected from loss as determined with use models lipopolysaccharide (LPS)-induced destruction. EEIG1-deficient macrophages markedly decreased RANKL- TNFα-mediated osteoclastogenesis...