A5041883322- Advanced X-ray Imaging Techniques
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Advanced Electron Microscopy Techniques and Applications
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Physics of Superconductivity and Magnetism
- Particle Accelerators and Free-Electron Lasers
- Crystallography and Radiation Phenomena
- Magnetic and transport properties of perovskites and related materials
- Advanced Condensed Matter Physics
- X-ray Spectroscopy and Fluorescence Analysis
- Glycosylation and Glycoproteins Research
- X-ray Diffraction in Crystallography
- Advanced NMR Techniques and Applications
- Laser-Plasma Interactions and Diagnostics
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Quantum and electron transport phenomena
- Marine Biology and Ecology Research
- Polar Research and Ecology
- Animal Virus Infections Studies
- Quantum many-body systems
- Chemical synthesis and alkaloids
Paul Scherrer Institute
2016-2025
University of Bern
2017
Swiss Light Source
2017
Institut de Biologie Structurale
2016
ETH Zurich
2006-2015
Center for Free-Electron Laser Science
2015
Universität Hamburg
2015
Scripps Research Institute
2007-2014
Joint Center for Structural Genomics
2014
Pedrini (Italy)
2013
The SwissFEL X-ray Free Electron Laser (XFEL) facility started construction at the Paul Scherrer Institute (Villigen, Switzerland) in 2013 and will be ready to accept its first users 2018 on Aramis hard branch. In following sections we summarize various aspects of project, including design soft branches accelerator, results performance simulations, details photon beamlines experimental stations, our commissioning results.
X-ray diffraction patterns from two-dimensional (2-D) protein crystals obtained using femtosecond pulses an free-electron laser (XFEL) are presented. To date, it has not been possible to acquire transmission individual 2-D due radiation damage. However, the intense and ultrafast generated by XFEL permit a new method of collecting data before sample is destroyed. Utilizing diffract-before-destroy approach at Linac Coherent Light Source, Bragg was acquired better than 8.5 Å resolution for two...
We present the main specifications of newly constructed Swiss Free Electron Laser, SwissFEL, and explore its potential impact on ultrafast science. In light recent achievements at current X-ray free electron lasers, we discuss territory for new scientific breakthroughs offered by SwissFEL in Chemistry, Biology, Materials Science, as well nonlinear
The nuclear magnetic resonance (NMR) structure of a central segment the previously annotated severe acute respiratory syndrome (SARS)-unique domain (SUD-M, for "middle SARS-unique domain") in SARS coronavirus (SARS-CoV) nonstructural protein 3 (nsp3) has been determined. SUD-M(513-651) exhibits macrodomain fold containing nsp3 residues 528 to 648, and there is flexibly extended N-terminal tail with 513 527 C-terminal flexible 649 651. As follow-up this initial result, we also solved...
HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not.The documents may come from teaching institutions in France abroad, public private centers.L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement recherche français étrangers, laboratoires publics privés. Hard x-ray...
Serial femtosecond crystallography (SFX) exploits extremely brief X-ray free-electron laser pulses to obtain diffraction data before destruction of the crystal. However, during pulse X-ray-induced site-specific radiation damage can occur, leading electronic state and/or structural changes. Here, we present a systematic exploration effect single-pulse duration and energy (and consequently different dose rates) on under typical SFX room-temperature experimental conditions. For first time in...
We present the results of measurements dc-magnetic susceptibility $\ensuremath{\chi}(T)$ and ${}^{23}\mathrm{Na}$-NMR response ${\mathrm{Na}}_{0.70}{\mathrm{CoO}}_{2}$ at temperatures between 50 340 K. The data suggest that for $T>75\mathrm{K},$ Co ions adopt an effective configuration ${\mathrm{Co}}^{3.4+}.$ reveals pronounced anomalies near 250 295 K, but no evidence magnetic phase transitions is found in $\ensuremath{\chi}(T).$ Our onset a dramatic change $3d$-electron spin dynamics This...
Wild-type strains of the protozoan ciliate Euplotes collected from different locations on coasts Antarctica, Tierra del Fuego and Arctic were taxonomically identified as morpho-species nobilii , based morphometric phylogenetic analyses. Subsequent studies their sexual interactions revealed that mating combinations Antarctic form stable pairs conjugant cells. These isolated shown to complete mutual gene exchange cross-fertilization. The biological significance this finding was further...
X-ray free-electron lasers (XFELs) offer a new avenue to the structural probing of complex materials, including biomolecules. Delivery precious sample XFEL beam is key consideration, as interest must be serially replaced after each destructive pulse. The fixed-target approach delivery involves depositing samples on thin-film support and subsequent serial introduction via translating stage. Some classes biological two-dimensional protein crystals, introduced supports, they require flat...
Membrane proteins arranged as two-dimensional crystals in the lipid environment provide close-to-physiological structural information, which is essential for understanding molecular mechanisms of protein function. Previously, X-ray diffraction from individual did not represent a suitable investigational tool because radiation damage. The recent availability ultrashort pulses free-electron lasers (XFELs) has now provided means to outrun Here, we report on measurements performed at Linac...
We propose a signal-to-noise criterion which predicts whether feature of given size and scattering strength, placed inside larger object, can be retrieved with two common X-ray imaging techniques: coherent diffraction projection microscopy.This criterion, based on how efficiently these techniques detect the scattered photons validated through simulations, shows in general that microscopy resolve smaller phase differences features than imaging.Our used to design optimized experiments perform...
The extension of transient grating spectroscopy to the x-ray regime will create numerous opportunities, ranging from study thermal transport in ballistic charge, spin, and energy transfer processes with atomic spatial femtosecond temporal resolution. Studies involving complicated split-and-delay lines have not yet been successful achieving this goal. Here we propose a novel, simple method based on Talbot effect for converging beams, which can easily be implemented at current free electron...
The Bernina instrument at the SwissFEL Aramis hard X-ray free-electron laser is designed for studying ultrafast phenomena in condensed matter and material science. Ultrashort pulses from an optical system covering a large wavelength range can be used to generate specific non-equilibrium states, whose subsequent temporal evolution probed by selective scattering techniques 2–12 keV. For that purpose, beamline equipped with elements which tailor beam size energy, as well pulse-to-pulse...
Structural information of the different conformational states two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in past by X-ray cryo-crystallography cryo-electron microscopy. However, these methods do not allow for structure determination most intermediate conformations. Recently, potential X-Ray Free Electron Lasers (X-FELs) tracking dynamics light-triggered processes pump-probe serial femtosecond crystallography demonstrated using...
We report the results of $^{87}\mathrm{Rb}$ NMR measurements on ${\mathrm{RbOs}}_{2}{\mathrm{O}}_{6}$, a member family superconducting pyrochlore-type oxides with critical temperature ${T}_{c}=6.4\phantom{\rule{0.3em}{0ex}}\mathrm{K}$. In normal state, nuclear spin-lattice relaxation time ${T}_{1}$ obeys Korringa-type relation ${T}_{1}T=\mathrm{const}$ and Knight shift is independent temperature, indicating absence strong magnetic correlations. ${T}_{1}^{\ensuremath{-}1}(T)$ exhibits tiny...