A5085696882- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Polymer Surface Interaction Studies
- Mechanical and Optical Resonators
- thermodynamics and calorimetric analyses
- Blood properties and coagulation
- Hydrogels: synthesis, properties, applications
- Near-Field Optical Microscopy
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Nanofabrication and Lithography Techniques
- Lipid Membrane Structure and Behavior
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neural Engineering
- Liquid Crystal Research Advancements
- Bone Tissue Engineering Materials
- Electrochemical sensors and biosensors
- Elasticity and Material Modeling
- Advanced Materials and Mechanics
- Polymer crystallization and properties
- Tendon Structure and Treatment
- Polysaccharides Composition and Applications
- Cell Adhesion Molecules Research
Hokkaido University
2015-2024
Tokyo University of the Arts
2024
The University of Tokyo
2024
Hokkaido University of Science
2018-2021
Inform (Germany)
2020
Sapporo Science Center
2008-2019
Tokyo Institute of Technology
1995-2011
Yokohama City University
1991
Cell fixation is an essential step to preserve cell samples for a wide range of biological assays involving histochemical and cytochemical analysis. Paraformaldehyde (PFA) has been widely used as cross-linking agent. It empirically recognized in gold standard protocol that the PFA concentration fixation, C PFA, 4%. However, it still not quantitatively clear how conventional optimized. Here, we investigated mechanical properties function by using atomic force microscopy scanning ion...
Fouling in membranes used for water treatment has been attributed to the presence of natural organic matter (NOM) water. There have reports recently on contribution hydrophilic fractions NOM (e.g., carbohydrate-like substances) fouling, but there is still little information about physicochemical interactions between and substances. In this study, affinity substances two different microfiltration (MF) was investigated by using atomic force microscopy (AFM) functionally modified microspheres....
Abstract Double‐network (DN) gels, a type of interpenetrating polymer network (IPN) consisting rigid and flexible components, exhibit two outstanding mechanical behaviors: yielding deformation the entire specimen in tensile tests quite high fracture energy tearing tests. In this study, atomic force microscope (AFM) measurements were conducted on DN gels to determine local Young's moduli immediately below surfaces E f usual molded m , compare modulus with bulk measured before after...
Kinetics of volume phase transition in poly(N-isopropylacrylamide) (NIPA) gels jumped from a low-temperature swollen to high-temperature shrunken was studied as functions NIPA monomer and crosslinker concentrations. We found for the first time clear kinematical boundary at which shrinking relaxation changes discontinuously by 102–104 times, that profile correlates with sol-gel line contour turbidity gels. A “morphological” characterizes emergence bubble formation on gel surface also...
The mechanosensitivity of neurons in the central nervous system (CNS) is an interesting issue as regards understanding neuronal development and designing compliant materials neural interfaces between external devices for treating CNS injuries disorders. Although neurite initiation from a cell body known to be first step towards forming functional network during or regeneration, less about how mechanical properties extracellular microenvironment affect neuritogenesis. Here, we investigated...
We present multi-frequency force modulation atomic microscopy (AFM) for mapping the complex shear modulus G* of living cells as a function frequency over range 50–500 Hz in same measurement time single-frequency measurement. The AFM technique enables us to reconstruct image maps rheological parameters, which exhibit frequency-dependent power-law behavior with respect G*. These quantitative measurements reveal large spatial variation this single cells. Moreover, we find that reconstructed...
This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 was cultured onto two-dimensional (2D) microplates, degradation the alginate sacrificial layer in system by addition lyase triggered NIH/3T3 cells self-fold microplates around HepG2 cells, and then 3D cell co-culture were spontaneously formed. Using this method, we can create...
Abstract During the developmental processes of embryos, cells undergo massive deformation and division that are regulated by mechanical cues. However, little is known about how embryonic change their properties during different cleavage stages. Here, using atomic force microscopy, we investigated stiffness in ascidian embryos from fertilised egg to stage before gastrulation. In both animal vegetal hemispheres, observed a Rho kinase (ROCK)-independent cell stiffening exhibited remarkable...
Stress relaxation of HepG2 cells was examined with an atomic force microscope (AFM). In the measurement, a loading applied to cell by AFM tip, and time series cantilever deflection signal measured at fixed position base displacement. The clearly observed on cells, well fitted stretched exponential function known as Kohlrausch–Williams–Watts (KWW) function, which is empirically employed represent dispersion processes system. stretching exponent parameter were estimated be ∼0.5 s 0.4–0.6,...
We measured stress and creep relaxations of mouse fibroblast cells arranged cultured on a microarray, by colloidal probe atomic force microscopy (AFM). A hydrophobic monolayer coating perfluorodecyltrichlorosilane (FDTS) the surface silica beads significantly reduced adhesion live cells, compared with untreated beads. The rheological behaviors were estimated averaging several relaxation curves AFM. Longer-time tailing both followed single power-law behavior over time scale 60 s, exponents in...
We investigated the apparent Young’s modulus, Em, of soft materials with surface tilt angle, as measured by colloidal probe atomic force microscopy (AFM). The AFM measurements polymer hydrogels and natural unfertilized eggs showed a clear universal behavior Em function θ, sample at local contact area. found that observed θ dependence was well fitted simple modified Hertz model, in which pressure distribution area follows conventional vertical component net balances loading force, remaining...
Fascin, an actin-bundling protein, is present in the filopodia and lamellipodia of growth cones. However, few studies have examined lamellipodial fascin because it difficult to observe. In this study, we evaluated fascin. We visualized actin meshwork live cones by super-resolution microscopy. Fascin was colocalized with lamellipodia. Ser39 a well-known phosphorylation site that controls binding filaments. Fluorescence recovery after photobleaching experiments confocal microscopy showed...
The number distribution of the elastic modulus fibroblast cells was successfully measured during early stages adhesion using an atomic force microscope (AFM) combined with a microarray as substrate, which allowed us to arrange and culture so that large could be in short time period. We confirmed deposited wells cultured for at least 12 h without any significant migration. Histograms Young's modulus, E, produced from curve measurements (n\cong300) 3–9 were well fitted log-normal function....
To investigate the mechanical properties of adherent cells under conditions where cell-to-cell interaction can be prevented and controlled, we used microcontact printing to pattern single without intercellular contacts measured them with a custom-built atomic force microscopy (AFM) system. The motorized AFM system measure individual over large spatial range, enabling measurement in microarray format. We tested fibroblast found that power-law their complex shear modulus is not significantly...
Background Dilated cardiomyopathy (DCM) is a major cause of heart failure in children. Despite intensive genetic analyses, pathogenic gene variants have not been identified most patients with DCM, which suggests that cardiomyocytes are solely responsible for DCM. Cardiac fibroblasts (CFs) the abundant cell type heart. They several roles maintaining cardiac function; however, pathological role CFs DCM remains unknown. Methods and Results Four primary cultured CF lines were established from...
As epithelial cells in vitro reach a highly confluent state, the often form microscale dome-like architecture that encloses fluid-filled lumen. The domes are stabilized by mechanical stress and luminal pressure. However, properties of remain poorly characterized at single-cell level. In this study, we used atomic force microscopy (AFM) to measure forming domes. AFM showed apparent Young's modulus was significantly higher when compared with surrounding monolayer. also stiffness tension were...
We measured the stress relaxation of mouse fibroblast NIH3T3 cells with an atomic force microscope (AFM) using a sharp silicon tip and silica bead radius ∼1 µm as indenter. The decay loading was clearly observed in at small initial ∼0.4 nN well fitted to stretched exponential function rather than single function. stretching exponent parameter ∼0.5 for both indenters, indicating that consisted multiple processes. time-domain AFM technique described this report allows us measure directly...