A5056468340- Wheat and Barley Genetics and Pathology
- Phytase and its Applications
- Plant Disease Resistance and Genetics
- Food composition and properties
- Plant Micronutrient Interactions and Effects
- Advanced Proteomics Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Horticultural and Viticultural Research
- Crop Yield and Soil Fertility
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Plant Pathogenic Bacteria Studies
- Food Allergy and Anaphylaxis Research
- Agricultural Productivity and Crop Improvement
- Genetics and Plant Breeding
- Plant Pathogens and Fungal Diseases
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Molecular Biology Techniques and Applications
- Plant nutrient uptake and metabolism
- Animal Genetics and Reproduction
- Plant pathogens and resistance mechanisms
- Selenium in Biological Systems
- Tree Root and Stability Studies
- Tissue Engineering and Regenerative Medicine
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2017-2023
Génétique, Diversité, Écophysiologie des Céréales
2010-2023
Université Clermont Auvergne
2018
Institut National de la Recherche Agronomique
2003-2012
Département Biologie et Amélioration des Plantes
1993-2007
Addenbrooke's Hospital
1997
University of Cambridge
1997
A molecular-marker map of bread wheat having many markers in common with other grasses the Gramineae family is a prerequisite for molecular level genetic studies and breeding this crop species. We have constructed restriction fragment length polymorphism maps A-, B-, D-genome chromosomes homoeologous group 2 hexaploid (Triticum aestivum L. em. Thell) using 114 F7 lines from synthetic x cross clones 11 libraries. Chromosomes 2A, 2B, 2D comprise 57, 60, 56 each spans about 200 cM. Comparisons...
The concentration and composition of the gliadin glutenin seed storage proteins (SSPs) in wheat flour are most important determinants its end-use value. In cereals, synthesis SSPs is predominantly regulated at transcriptional level by a complex network involving least five cis-elements gene promoters. high-molecular-weight subunits (HMW-GS) encoded two tightly linked genes located on long arms group 1 chromosomes. Here, we sequenced annotated HMW-GS promoters 22 electrophoretic alleles to...
Summary The quality of wheat grain is mainly determined by the quantity and composition its storage proteins ( GSP s). Grain consist low‐ high‐molecular‐weight glutenins LMW ‐ GS HMW , respectively) gliadins. synthesis these essentially regulated at transcriptional level availability nitrogen sulfur. regulation network has been extensively studied in barley where BLZ 1 2, members basic leucine zipper bZIP ) family, activate hordeins. To date, wheat, only ortholog Storage Protein Activator...
Wheat proteins and specially gluten have been well studied are closely associated with baking products. Amphiphilic (proteins that soluble using nonionic detergent Triton X-114 ) also play an important role in wheat quality. Some of them, like puroindolines, lipid binding proteins, strongly linked to dough foaming properties fine crumb texture. However many amphiphilic still unknown both their physiological technological functions remain be analysed. In order explore these proteomic analysis...
Wheat kernel texture, a major trait determining the end-use quality of wheat flour, is mainly influenced by puroindolines. These small basic proteins display in vitro lipid binding and antimicrobial properties, but their cellular functions during grain development remain unknown. To gain an insight into biological function, comparative proteome analysis two near-isogenic lines (NILs) bread Triticum aestivum L. cv. Falcon differing presence or absence puroindoline-a gene (Pina) hardness, was...
Wheat presents an important genetic diversity that could be useful to look for cultivars with reduced allergencity. ω5-Gliadins have been described as major allergens wheat allergic patients suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA) and some cases of chronic urticaria (U). Our objective was study the influence variability at Gli-B1 locus encoding ω5-gliadins on reactivity IgE antibodies these patients. We selected expressing 13 alleles including a wheat/rye...
Grain storage proteins (GSPs) quantity and composition determine the end-use value of wheat flour. GSPs consists low-molecular-weight glutenins (LMW-GS), high-molecular-weight (HMW-GS) gliadins. GSP gene expression is controlled by a complex network DNA-protein protein-protein interactions, which coordinate tissue-specific protein during grain development. The regulatory has been most extensively studied in barley, particularly two transcription factors (TFs) DNA binding with One Finger...
The expression of cereal grain storage protein (GSP) genes is controlled by a complex network transcription factors (TFs). Storage activator (SPA) major TF acting in this but its specific function wheat (Triticum aestivum L.) remains to be determined. Here we generated an RNAi line which the three SPA homoeologs was reduced. In and null segregant analyzed GSP accumulation regulatory under two regimes nitrogen availability. We show that down regulation decreases concentration at maturity low...