A5050505917- Cardiac electrophysiology and arrhythmias
- Cardiac pacing and defibrillation studies
- Cardiac Arrest and Resuscitation
- DNA Repair Mechanisms
- Heart Failure Treatment and Management
- Cardiac Arrhythmias and Treatments
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Thermal Regulation in Medicine
- Photosynthetic Processes and Mechanisms
- Cardiovascular Function and Risk Factors
- Ion channel regulation and function
- Cardiac Ischemia and Reperfusion
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- Intracranial Aneurysms: Treatment and Complications
- RNA Research and Splicing
- Cerebrovascular and Carotid Artery Diseases
- Respiratory Support and Mechanisms
- Cardiac and Coronary Surgery Techniques
- Microtubule and mitosis dynamics
- Mobile Health and mHealth Applications
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Aortic aneurysm repair treatments
Semmes Murphey Foundation
2025
University of Tennessee Health Science Center
2024
Medtronic (United States)
2000-2023
St. Anna Children's Cancer Research Institute
2023
St Anna Children's Hospital
2023
Vienna Biocenter
2018-2022
University of Vienna
2018-2022
Max Perutz Labs
2016-2022
Universität Hamburg
2022
Manchester University NHS Foundation Trust
2020
To elucidate the molecular nature of evolutionary changes telomeres in plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) these plants. The unusually long telomere repeat unit Allium plants (12 nt) allowed us identify TRs transcriptomic data representative species genus. Orthologous were then identified Asparagales harbouring DNA composed TTAGGG (human type) or TTTAGGG (Arabidopsis-type) repeats. Further, across land phylogeny, including common model plants, crop...
In the current meiotic recombination initiation model, SPO11 catalytic subunits associate with MTOPVIB to form a Topoisomerase VI-like complex that generates DNA double strand breaks (DSBs). Four additional proteins, PRD1/AtMEI1, PRD2/AtMEI4, PRD3/AtMER2 and plant specific DFO are required for DSB formation. Here we show (i) PRD1 provide link between sub-complex other (ii) PRD3/AtMER2, while localized axis, does not assemble canonical pre-DSB but establishes direct DSB-forming resection...
The epidemiological, clinical, and societal implications of the heart failure (HF) epidemic cannot be overemphasized. Approximately half all HF patients have with preserved ejection fraction (HFpEF). HFpEF is largely a syndrome elderly, aging population, proportion expected to grow. Currently, there no drug known improve mortality or hospitalization risk for these patients. Besides hospitalization, it imperative realize that significant impairment in their functional capacity quality life on...
Fanconi anemia (FA) is a human autosomal recessive disorder characterized by chromosomal instability, developmental pathologies, predisposition to cancer, and reduced fertility. So far, 19 genes have been implicated in FA, most of them involved DNA repair. Some are conserved across higher eukaryotes, including plants. The Arabidopsis thaliana genome encodes homolog the D2 gene (FANCD2) whose function repair not yet fully understood. Here, we provide evidence that AtFANCD2 required for...
Ribosomal RNA genes are arranged in large arrays with hundreds of rDNA units tandem. These highly repetitive DNA elements pose a risk to genome stability since they can undergo nonallelic exchanges. During meiosis, double-strand breaks (DSBs) induced as part the regular program generate gametes. Meiotic DSBs initiate homologous recombination (HR), which subsequently ensures genetic exchange and chromosome disjunction. In Arabidopsis (Arabidopsis thaliana), we demonstrate that all 45S become...
Abstract Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted multiple, highly repetitive rDNA genes, encoding catalytic ribosomal core RNAs which transcribed from 45S units. Their precise sequence information and organization remain uncharacterized. Here, using a combination long- short-read sequencing technologies we assemble contigs Arabidopsis NOR2 domain. We identify several...
Rationale : Cardioprotective pathways may involve a mitochondrial ATP-sensitive potassium (mitoK ATP ) channel but its composition is not fully understood. Objective We hypothesized that the mitoK contains sulfonylurea receptor (SUR)2 regulatory subunit and aimed to identify molecular structure. Methods Results Western blot analysis in cardiac mitochondria detected 55-kDa SUR2 (mitoSUR2) short form, 2 additional forms (28 68 kDa), 130-kDa long form. RACE (Rapid Amplification of cDNA Ends)...
INTRODUCTION: Woven Endobridge (WEB) is a safe and effective intrasaccular flow disruptor with over 80% adequate occlusion rates. However, complete rates remain around 50-60%. Recent studies showed that sizing modifications lateral compression correlate METHODS: This single center retrospective study of all cerebral aneurysms treated WEB devices from August 2015 to December 2023 follow-up data. We collected basic demographic, as well The aneurysm entry angle was calculated by measuring the...
Refractory headache is a common and frequently disabling disorder, affecting 2-12 million patients each year in the US alone. This case series provides initial insight into safety effectiveness of lidocaine infusions middle meningeal artery (MMAL) for treatment refractory headache. A retrospective review prospectively maintained database was conducted to identify undergoing MMAL at two institutions. Baseline demographics, procedural metrics, measures were tracked over 3 month period....
Objective Determine the effects of hypothermia on defibrillation energy requirements and cardiac electrophysiology. Design Prospective randomized acute intervention trial. Setting Medical center animal laboratory. Subjects Fifteen domestic farm swine. Interventions Swine were to a group (n = 8) or control 7). All animals instrumented with transvenous system connected defibrillator that delivers biphasic-truncated waveform. Values for measured at baseline (normothermia, 38–40°C) during...
Abstract Background Remote monitoring (RM) improves patient outcomes. App‐based RM is a novel technology that enables transmission of implantable device data using smart devices. Limited exist on the impact age and sex use app‐based RM. Objective To examine proportion pacemaker patients who activated RM, time from order to activation, follow‐up adherence per guidelines. Methods A retrospective analysis was performed deidentified U.S. enrolled in Medtronic CareLink database with an monitor...
Patients with obstructive sleep apnea who receive drug therapy for cardiovascular disease may experience resistant hypertension, arrhythmias, or more severe heart failure, and many of the drugs used to treat these conditions are substrates P-glycoprotein (P-gp) transporters. Therefore, we sought determine if intermittent hypoxia, which mimics apnea, would upregulate myocardial hepatic P-gp expression Abcb1a Abcb1b messenger RNA (mRNA) (genes that encode P-gp) in an animal model.Prospective,...
Regional Hyperkalemia and Defibrillation. Introduction: Increased spatial electrical heterogeneity has heen associated with impaired defibrillation efficacy. The current study investigated the relationship between efficacy by manipulating heterogeneity. Methods Results: We increased infusing potassium chloride (2 to 4 mEq/hour) or placebo In left anterior descending artery in 13 pentobarbital anesthetized swine. Electrophysiologic measurements at five myocardial sites energy requirement...
To examine the role of allopurinol as a cardioprotectant during coronary artery bypass graft (CABG) surgery.A search MEDLINE (1966-October 2002) was performed using following terms: allopurinol, xanthine oxidase, oxygen free radical, and bypass. References evaluated were limited to English-language human studies, yielding 41 citations, 13 which found suitable. The 5 largest studies are discussed.Multiple with various doses have effects on outcomes in CABG patients. These that can reduce...
It is clear that ischemia inhibits successful defibrillation by altering regional electro-physiology. However, the exact mechanisms are unclear. This study investigated whether gap junction inhibition increases biphasic shock thresholds (DFT). Sixteen swine were instrumented with a mid-left anterior descending (LAD) perfusion catheter for infusion of 0.5 mM/h heptanol ( n = 8) or saline 8). DFT values and effective refractory periods (ERP) at five myocardial sites determined. Regional...
Inappropriate internal cardioverter defibrillator (ICD) therapies may result from T-wave oversensing (TWOS) during exertion in children. The aim of this study was to evaluate the utility an exercise treadmill test predict inappropriate ICD secondary TWOS.Eligible pediatric recipients underwent exercise-stress testing with concomitant evaluation all intracardiac electrograms. Double counting at a programmed sensitivity 0.3 mV considered indicative TWOS. Patients were prospectively followed...
Heart failure (HF) affects approximately 26 million people worldwide. With an aging global population, innovative approaches to HF evaluation and management are needed cope with the worsening epidemic. The aim of Real-Life Multimarker Monitoring in Patients Failure (REALIsM-HF) study (NCT03507439) is evaluate a composite instrument comprising remote, real-time, activity-monitoring devices combined daily electronic patient-reported outcome (ePRO) items patients who have been hospitalized for...
The incidence of seroma after abdominoplasty is accepted as approximately 10% (with a range) in the literature. Progressive tension sutures (PTS) have arisen means reducing seroma, however there are conflicting data regarding their efficacy.The primary aim this study was to describe postabdominoplasty setting drainless with PTS.A retrospective chart review performed all abdominoplasties (n = 454) during 20-year period. At halfway point time frame, technique changed from use 2 drains PTS...
Introductory Paragraph In many species, including Arabidopsis, heterochromatin often comprises repetitive DNA elements, such as arrays of ribosomal (rDNA). Repetitive regions pose a risk in meiosis since recombination between them can lead to gross genomic rearrangements. However, meiotic at rDNA and other heterochromatic repeat is blocked by not well understood mechanisms. Here, we have identified RAD21.2, an α-kleisin subunit cohesin, repressor the Arabidopsis. We show that RAD21.2...
Background —This study determined whether dispersion of conduction velocity, refractoriness, or excitability increases biphasic shock defibrillation energy requirements (DERs). Methods and Results —Twenty-four swine were instrumented with a mid-LAD perfusion catheter for regional infusion lidocaine 0.75 mg · kg −1 h (n=7), low-dose d -sotalol (0.16 ) (n=4), high-dose (0.5 (n=6), saline (n=7). Effective refractory periods (ERPs) at 5 myocardial sites, velocity was in LAD-perfused -nonperfused...
An experimental model of conduction velocity (CV) and refractory period dispersion was established to determine which variable is a determinant myocardial vulnerability. Anesthetized swine were instrumented with left anterior descending coronary artery catheter for regional infusion lidocaine (n = 6), low-dose d-sotalol 4), high-dose or saline create in CV (lidocaine), refractoriness (d-sotalol), neither (saline). Ventricular fibrillation thresholds (VFTs) periods determined at five sites...