A5038735779- Breast Lesions and Carcinomas
- Cancer and Skin Lesions
- Breast Cancer Treatment Studies
- Salivary Gland Tumors Diagnosis and Treatment
- AI in cancer detection
- Soft tissue tumor case studies
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- RNA modifications and cancer
- Cardiac tumors and thrombi
- Estrogen and related hormone effects
- Cancer Immunotherapy and Biomarkers
- Urologic and reproductive health conditions
- Genetic factors in colorectal cancer
- Pancreatic and Hepatic Oncology Research
- Advanced Breast Cancer Therapies
- Fibroblast Growth Factor Research
- Inflammatory Biomarkers in Disease Prognosis
- Immunotherapy and Immune Responses
- Thyroid Cancer Diagnosis and Treatment
- Meta-analysis and systematic reviews
- Delphi Technique in Research
- Immune cells in cancer
- Medical Imaging and Pathology Studies
Singapore General Hospital
2014-2023
Royal Hampshire County Hospital
2003
Angiosarcomas are rare, clinically aggressive tumors with limited treatment options and a dismal prognosis. We analyzed angiosarcomas from 68 patients, integrating information multiomic sequencing, NanoString immuno-oncology profiling, multiplex immunohistochemistry immunofluorescence for tumor-infiltrating immune cells. Through whole-genome sequencing (n = 18), 50% of the cutaneous head neck exhibited higher tumor mutation burden (TMB) UV mutational signatures; others were mutationally...
Aim To determine the frequency of MED12 mutations in a series 112 breast phyllodes tumours, and to correlate findings with clinicopathological parameters survival outcomes. Methods Phyllodes tumours from Department Pathology, Singapore General Hospital, were classified into benign, borderline malignant categories. Genomic DNA formalin-fixed paraffin-embedded was extracted, purified subjected ultra-deep-targeted amplicon sequencing across exon 2 gene. Sequencing performed on Illumina MiSeq...
Fibroepithelial lesions (FELs) are a heterogeneous group of tumours comprising fibroadenomas (FAs) and phyllodes (PTs). Here we used 16-gene panel that was previously discovered to be implicated in pathogenesis progression, characterise large international cohort FELs via targeted sequencing. The study comprised 303 (38%) FAs 493 (62%) PTs which were contributed by the International Consortium. There 659 (83%) Asian 109 (14%) non-Asian FELs, while ethnicity rest unknown. Genetic aberrations...
Recent reports have identified recurrent MED12 somatic mutations in fibroadenomas and phyllodes tumours. The frequency type of were noted to be similar those uterine leiomyomas. We aimed investigate protein expression MED12, correlating it mutational status oestrogen receptors (ER).Immunohistochemistry was performed on a total 232 fibroepithelial lesions (100 fibroadenomas, 132 tumours) diagnosed at the Department Pathology, Singapore General Hospital using ERα ERβ antibodies. Expressions...
The hierarchy of evidence is a fundamental concept in evidence-based medicine, but existing models can be challenging to apply laboratory-based health care disciplines, such as pathology, where the types and contexts are significantly different from interventional medicine. This project aimed define comprehensive complementary framework new levels for evaluating research tumor pathology-introducing novel Hierarchy Research Evidence Tumor Pathology collaboratively designed by pathologists...
Aims Breast phyllodes tumours (PTs) are a rare subset of fibroepithelial neoplasms categorised into benign, borderline, and malignant grades according to the World Health Organization (WHO) Classification Tumours (WCTs). In this report, we developed an evidence gap map (EGM) based on literature cited in PT chapter 5th edition breast WCT order identify knowledge research gaps PT. Methods A framework was first established where dimensions EGM were defined as categories tumour descriptors,...
Abstract Malignant phyllodes tumors (PT) are rare aggressive fibroepithelial neoplasms with high metastatic potential and lack effective therapy. We established a patient-derived xenograft (PDX) cell line model (designated MPT-S1) of malignant PT which demonstrated clinical response to pazopanib. Whole exome sequencing identified somatic mutations in TP53 , RB1 MED12, KMT2D . Immunohistochemistry genomic profiles the tumor, PDX were concordant. In keeping observation, pazopanib reduced...
Characterising the factors responsible for metastatic triple-negative breast cancer (TNBC) is of significant importance, considering its high mortality rate and scant data. In this study, we evaluated characteristics, clinical behaviour role biomarkers (androgen receptor (AR), oestrogen beta (ERβ) p53) in TNBC.Immunohistochemistry was performed AR, ERβ p53 on 125 primary TNBCs with known metastasis correlated clinicopathological parameters outcome. AR mRNA profiling also carried out 34...
Introduction: A well-validated diagnostic assay with curated biomarkers complements clinicopathological factors to facilitate early diagnosis and ensure timely treatment delivery. This study focuses on an Asian-centric cancer designed thoroughly validated against commercially available standard references a cohort of over 200 clinical specimens spanning 12 diverse types. Methods: The uses hybrid-capture probes capable profiling DNA aberrations from 572 cancer-related genes 91 RNA fusion...
Context.— Mitotic count is an important histologic criterion for grading and prognostication in phyllodes tumors (PTs). Counting mitoses a routine practice pathologists evaluating neoplasms, but different microscopes, variable field selection, areas have led to possible misclassification. Objective.— To determine whether 10 high-power fields (HPFs) or whole slide mitotic counts correlated better with PT clinicopathologic parameters using digital pathology (DP). We also aimed find out this...
Juvenile fibroadenomas (JFA) are biphasic fibroepithelial lesions (FEL) usually occurring in adolescent female patients. Giant (G) JFA, like other FEL, may exhibit prominent pseudoangiomatous stromal hyperplasia (PASH)-like change. We sought to determine clinicopathological and molecular characteristics of GJFA with without PASH.Archives were searched for cases (1985-2020). All stained androgen receptor (AR), beta-catenin, CD34 progesterone (PR). Cases sequenced using a custom 16-gene panel...