Abstract Background Karst caves are considered as extreme environments with nutrition deficiency, darkness, and oxygen deprivation, they also the sources of biodiversity metabolic pathways. Microorganisms usually involved in formation maintenance cave system through various activities, indicators changes environment influenced by human. Zhijin is a typical attracts tourists China. However, bacterial diversity composition still unclear. The present study aims to reveal potential impact...

Stenotrophomonas maltophilia ( S. ) is an emerging opportunistic pathogen that exhibits resistant to a majority of commonly used antibiotics. Phages have the potential serve as alternative treatment for infections. In this study, lytic phage, A1432, infecting YCR3A-1, was isolated and characterized from karst cave. Transmission electron microscopy revealed phage A1432 possesses icosahedral head shorter tail. Phage demonstrated narrow host range, with optimal multiplicity infection 0.1. The...

Evaluating the synergistic environmental and health effects of energy control policies is essential for scientifically informed policy optimization. Given that air quality affected by factors such as meteorological conditions economic growth, relying solely on monitoring data insufficient to assess impacts these accurately. To objectively examine both environment public health, this study introduces CEEMDAN-OA-SVR-DID model from a predictive perspective. The forecasts PM2.5 concentrations...

Magnesium plays an important role in regulating the growth and quality of tea trees. However, effect magnesium regulation on changes aroma tree leaves remains unknown. In this study, volatile compounds under different concentrations were determined to obtain characteristic that changed significantly their odor characteristics explore leaves. The results showed enhanced content 15 leaves, especially heptyl formate t-geraniol. mainly five kinds characteristics, such as fruity, green, floral,...

Viral infection can regulate the cell cycle, thereby promoting viral replication. Hijacking and altering cycle are important for virus to establish maintain latent infection. Previously, Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) latently infected P8-Se301-C1 cells, which grew more slowly than Se301 cells interfered with homologous SeMNNPV superinfection, were established. However, effects of superinfection baculoviruses on progression remain unknown. In this study, profiles...

Pseudomonas syringae is a gram-negative plant pathogen that infects plants such as tomato and poses threat to global crop production. In this study, novel lytic phage infecting P. pv. DC3000, named D6, was isolated characterized from sediments in karst cave. The latent period of D6 found be 60 min, with burst size 16 plaque-forming units per cell. Phage stable at temperatures between 4 40 °C but lost infectivity when heated 70 °C. Its unaffected pH 6-10 became inactivated ≤ 5 or ≥ 12. genome...

Bean beetle (Callosobruchus maculatus) exhibits clear phenotypic plasticity depending on population density; However, the underlying molecular mechanism remains unknown. Compared to low-density individuals, high-density individuals showed a faster terminal oocyte maturity rate. Four insulin-like peptide (ILP) genes were identified in bean beetle, which had higher expression levels head than thorax and abdomen. The density could regulate of CmILP1-3, CmILP2-3, CmILP1 as well CmILP3 head,...

Abscisic acid (ABA) plays important roles in positively or negatively regulating plant disease resistance to pathogens. Here, we reassess the role of endogenous and exogenous ABA by using: 35S::ABA2, a previously reported transgenic Arabidopsis line with increased levels; aba2-1, ABA2 mutant reduced application ABA. We found that bacterial susceptibility promoted was suppressed 35S::ABA2 plants. The aba2-1 plants displayed elevated levels, respectively, flagellin peptide (flg22)-induced...

Abstract Background The fungal communities inhabiting natural Ophiocordyceps sinensis play critical ecological roles in alpine meadow ecosystem, contribute to infect host insect, influence the occurrence of O. , and are repertoire potential novel metabolites discovery. However, a comprehensive understanding remain elusive. Therefore, present study aimed unravel using combination high-throughput sequencing culture-dependent approaches. Results A total 280,519 high-quality sequences, belonging...

Acid mine drainage (AMD) is usually acidic (pH < 4) and contains high concentrations of dissolved metals metalloids, making AMD a typical representative extreme environments. Recent studies have shown that microbes play key role in bioremediation, secondary metabolite biosynthetic gene clusters (smBGCs) from are important resources for the synthesis antibacterial anticancer drugs. Here, 179 samples 13 mineral types were used to analyze putative novel microorganisms metabolites Among 7,007...

The mitochondrial genome of Eysarcoris guttigerus has been decoded using Illumina sequencing. mitogenomic size is 15,368 bp with 42.97% A, 33.90% T, 13.22% C, and 9.90% G. It encoded 13 protein-coding genes, two ribosomal RNA, 22 transfer RNAs. Phylogenetic tree showed that genus had a closer relationship the Carbula than rest 20 genera.

The mitochondrial genome of Melanostoma orientale has been decoded by Illumina sequencing. mitogenomic size is 16,229 bp with 40.96% A, 40.29% T, 10.60% C, and 8.15% G. It encoded 13 protein-coding genes, 2 ribosomal RNA, 22 transfer RNAs. phylogenetic tree showed that 10 species Syrphidae, belonging to six genera, were clustered into two clades. This the first for genus Melanostoma.

A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China. The isolate grew optimally on R2A agar at 25 °C, pH 8.0 with 0.5 % (w/v) NaCl. Phylogenetic analysis based 16S rRNA gene sequences showed that belonged to the genus Arthrobacter most closely related methylotrophus TGAT (98.3 sequence similarity), alkaliphilus LC6T (97.7 %) ramosus...

Alien chromosome substitution lines are treated as vital germplasms for breeding and genetic mapping. Previously, a whole set of nine Brassica rapa-oleracea monosonic alien addition (MAALs, C1-C9) was established in the background natural B. napus genotype “Oro”, after restituted rapa (RBR) Oro realized. Herein, monosomic line with one C1 (Cs1) RBR complement selected progenies MAAL RBR, by PCR amplification specific gene markers fluorescence situ hybridization. Cs1 exhibited plant...

After serially undiluted passage of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), persistently infected Se301 cells were established. A cell strain, in which no polyhedra or viral particles observed, was cloned and designated P8-Se301-C1. The P8-Se301-C1 are morphologically similar to but grow slower than they can homologously interfere with SeMNPV. PCR analysis showed that SeMNPV ie-0 polyhedrin genes present DNA polymerase orf67 absent P8-Se301-C1, suggesting the harbour...

Garden cress, Lepidium sativum L., is not only an important vegetable which cultivated in the entire world, but also a widely used folk medicine for treatment of hyperactive airways disorders. However, as member Brassicaceae, few studies have been carried out on its phylogenetic relationship with other Brassicaceae members. Herein, complete chloroplast (cp) genome garden cress wa deciphered by combination Illumina Hiseq and PacBio Platform after extracting cp DNA. The showed typically...