A5060540390- Photodynamic Therapy Research Studies
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Nanoplatforms for cancer theranostics
- Ion channel regulation and function
- Nitric Oxide and Endothelin Effects
- Circadian rhythm and melatonin
- Pancreatitis Pathology and Treatment
- Porphyrin and Phthalocyanine Chemistry
- Hypothalamic control of reproductive hormones
- Photochromic and Fluorescence Chemistry
- Redox biology and oxidative stress
- Ion Channels and Receptors
- Neonatal Health and Biochemistry
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Growth Hormone and Insulin-like Growth Factors
- Protein Kinase Regulation and GTPase Signaling
- Photosynthetic Processes and Mechanisms
- bioluminescence and chemiluminescence research
- Neurobiology and Insect Physiology Research
- Chemical Synthesis and Analysis
- Pancreatic and Hepatic Oncology Research
- Light effects on plants
- Biochemical effects in animals
Beijing Normal University
2016-2025
Institute of Cell Biology
2016-2022
China Agricultural University
1997-2016
Hokkaido University
1997
Yale University
1992-1996
University of Cambridge
1989-1993
Atherosclerosis is a chronic inflammatory disease that can cause acute cardiovascular events. Activation of the NOD-like receptor family, pyrin domain containing protein 3 (NLRP3) inflammasome enhances atherogenesis, which links lipid metabolism to sterile inflammation. This study examines impact an endogenous metabolite, namely ketone body 3-hydroxybutyrate (3-HB), on mouse model atherosclerosis. It found daily oral administration 3-HB significantly ameliorate Mechanistically, reduce M1...
Photodynamic agents, due to their photon‐dependent selective activation, can selectively activate a number of physiological processes and may directly modulate signal transduction in cells including pancreatic acinar cells. Activation the photodynamic agent sulphonated aluminium phthalocyanine (SALPC) triggered recurrent cytosolic calcium ([Ca 2+ ] i ) spiking The photodynamically could be blocked by phosphatidylinositol‐specific phospholipase C (PI‐PLC) inhibitor U73122, but not...
In acute pancreatitis, histones are released by infiltrating neutrophils, but how modulate pancreatic acinar cell function has not been investigated. We have examined histone modulation of rat acini and tumor AR4-2J calcium imaging. Histones were found to no effect on in blocked oscillations induced cholecystokinin or acetylcholine. Both mixed (Hx) individual (H1, H2A, H2B, H3, H4) AR4-2J. RT-PCR Western blot verified the expression histone-targeted Toll-like receptor (TLR) 2, 4 9....
To know whether thioredoxin 1 (Trx1) works for an antioxidant defense mechanism in atherosclerosis, the effect of Trx1 on release monocyte chemoattractant protein-1 (MCP-1), a potent recruitment and accumulation monocytes/macrophages intima artery vessel, was investigated human endothelial-like EA.hy 926 cells. It found that overexpression suppressed, whereas knockdown endogenous enhanced, oxidized low-density lipoprotein (oxLDL)-stimulated MCP-1 expression also observed depletion greatly...
Abstract Little is known about the blood‐feeding physiology of arbovirus vector Aedes aegypti although this type mosquito to transmit infectious diseases dengue, Zika, yellow fever, and chikungunya. Blood feeding in female A. essential for egg maturation transmission disease agents between human subjects. Here, we identify sulfakinin receptor gene SKR from genome show that expressed at different developmental stages varied anatomical localizations adult (at three days after eclosion), with...
Plasma membrane-delimited generation of singlet oxygen by photodynamic action with photosensitizer sulfonated aluminum phthalocyanine (SALPC) activates cholecystokinin 1 receptor (CCK1R) in pancreatic acini. Whether CCK1R retains such photooxidative activation properties other environments is not known. Genetically encoded protein photosensitizers KillerRed or mini generator (miniSOG) were expressed acinar tumor cell line AR4-2J, CCK1R, miniSOG HEK293 CHO-K1 cells. Cold light irradiation (87...
PRL release from rat lactotrophs in response to TRH is Ca2+ dependent. TRH-induced inhibited either after repeated pulses of or the presence dopamine. TRH, however, generates increases intracellular concentrations ([Ca2+]i) both conditions. Calcium/calmodulin-dependent protein kinase-II (CaM kinase-II) a ubiquitous enzyme implicated secretion. To determine whether down-regulation CaM activity caused lack responsiveness [Ca2+]i, we measured generation calcium/calmodulin-independent kinase...
Sulphonated aluminium phthalocyanine (SALPC) photodynamic action induces amylase secretion and permanent calcium oscillation in rat pancreatic acinar cells, because of the activation phospholipase C or signalling proteins upstream. The aim present study was to investigate involvement muscarinic acetylcholine cholecystokinin (CCK) receptors. Muscarinic receptor antagonist atropine (10 μ M ) blocked induced by bethanechol (100 ), CCK 1 ( S )‐ N...
Abstract Effects of photodynamic action gadolinium porphyrin‐like macrocycle B (PLMGdB) on cytosolic Ca 2+ concentration, [Ca ] c , was investigated in isolated rat pancreatic acini. The PLMGdB alone or light (2 min) had no effect . Cell‐bound upon brief (0.5–2.0 activation triggered recurrent spikes At lower concentration (100 nM) the continued during whole period monitoring a higher 500 nM, for first 40 min, followed by gradual increase basal which smaller were superimposed. 1, 20 after...
In contrast to reversible activation by agonist, cholecystokinin 1 receptor (CCK1R) is permanently activated singlet oxygen generated in photodynamic action, with sulphonated aluminium phthalocyanine or genetically encoded mini generator (miniSOG) as photosensitizer. these works, a halogen light source was used power action. For possible vivo application of CCK1R physiology, bearing cumbersome light-delivery device connected an external experimental animals might interfere their behavior....
Although methionine sulfoxide reductase (Msr) is known to modulate the activity of multiple functional proteins, roles Msr in pancreatic stellate cell physiology have not been reported. In present work we investigated expression and function freshly isolated cultured rat cells. was determined by RT-PCR, Western blot immunocytochemistry. over-expression achieved transfection with adenovirus vectors. Pancreatic cells were co-cultured acinar AR4-2J monolayer culture. monitored Fura-2 calcium...
Cholecystokinin 1 receptor (CCK1R) is activated by singlet oxygen (1O2) generated in photodynamic action with sulphonated aluminum phthalocyanine (SALPC) or genetically encoded protein photosensitizer (GEPP) KillerRed mini generator (miniSOG). A large number of GEPP varied 1O2 quantum yields have appeared recently; therefore, the present work, efficacy different to photodynamically activate CCK1R was examined, as monitored Fura-2 calcium imaging. KillerRed, miniSOG, miniSOG2, (SOPP),...