Hepcidin, a key regulator of iron metabolism, decreases intestinal absorption and its release from macrophages. Iron, anemia, hypoxia, inflammation were reported to influence hepcidin expression. To investigate regulation the expression other iron-related genes, we manipulated erythropoietic activity in mice. Erythropoiesis was inhibited by irradiation or posttransfusion polycythemia stimulated phenylhydrazine administration erythropoietin. Gene genes (hemojuvelin, DMT1, ferroportin,...

Mutations in HFE, the most common cause of hereditary hemochromatosis, lead to iron overload. The overload is characterized by increased uptake due lower levels hepatic, regulatory hormone hepcidin. HFE was cloned 21 years ago, but signaling pathway still unknown. Because bone morphogenetic protein (BMP) impaired patients with and interaction BMP type I receptor ALK3 suggested vitro, vivo experiments were performed. In vivo, hepatocyte-specific Alk3-deficient control mice injected either...

Matriptase-2 (TMPRSS6) is an important negative regulator of hepcidin expression; however, the effects iron overload or accelerated erythropoiesis on liver TMPRSS6 protein content in vivo are largely unknown. We determined plasma membrane-enriched fractions homogenates by immunoblotting, using a commercial antibody raised against catalytic domain TMPRSS6. Plasma were obtained centrifugation at 3000 g and washing. was detected fraction as 120 kDa full-length both mice rats. Feeding...

The purpose of the study was to investigate expression ferroportin protein following treatments that affect systemic hepcidin. Administration erythropoietin C57BL/6J mice decreased hepcidin expression; it also increased heart content, determined by immunoblot in membrane fraction, approximately 200% control values. This increase is very probably caused a decrease expression, accordance with classical regulation However, could apparently be overridden changes non-heme iron content since...

Introduction Hemojuvelin (Hjv) is a key component of the signaling cascade that regulates liver hepcidin (Hamp) expression. The purpose this study was to determine Hjv protein levels in mice and rats subjected iron overload deficiency. Methods C57BL/6 were injected with (200 mg/kg); deficiency induced by feeding an iron-deficient diet, or repeated phlebotomies. Erythropoietin (EPO)-treated administered recombinant EPO at 50 U/mouse. Wistar (1200 mg/kg), fed diet. determined immunoblotting,...

Matriptase-2, a membrane protein encoded by the Tmprss6 gene, is negative regulator of hepcidin expression. Although matriptase-2 has been proposed to cleave hemojuvelin, we have recently found decreased hemojuvelin levels in -/- mice. The purpose this study was confirm observation determining another strain mice with disrupted and determine effect deficiency on expression other proteins participating bone morphogenetic signal transduction. Mask mice, which lack proteolytic domain...

Tmprss6-mutated mask mice display iron deficiency anemia and high expression of hepcidin. The aim the study was to determine effect erythropoietin administration on proteins participating in control homeostasis liver spleen C57BL/6 mice. Administration for four days at 50 IU/mouse/day increased hemoglobin hematocrit mice, no such increase seen Erythropoietin decreased hepcidin but not treatment significantly size both Furthermore, splenic Fam132b, Fam132a Tfr2 mRNA content. At protein level,...

Cancer is known to be an important cause of anaemia due several factors including iron deficiency and inflammation. Hepcidin, a key regulator metabolism, up-regulated by inflammatory stimuli such as interleukin 6, decreased deficiency, enhanced erythropoiesis hypoxia. It supposed play crucial role in changes metabolism chronic disease, which characterized sequestering macrophages decreasing its availability for red blood cell production. To study the effect tumour growth on hepcidin...

Iron overload causes tissue damage in the liver, but its initial effects at molecular and cellular level are not well understood. Epithelial cadherin (E-cad) is a major adhesion protein adherens junctions associated with several signal transduction pathways. Dysfunction of E-cad instability junctions, which leads to cell invasion, migration, carcinogenesis. We found liver samples from iron-overloaded mice that apparent mass was reduced 125 115 kDa sodium dodecyl sulphate polyacrylamide gel...

This study used biochemical markers to assess contamination at two contaminated sites (Králíky and Lichkov) one control site (Červená Voda) on the River Tichá Orlice, a left-side tributary of Elbe. The brown trout ( Salmo trutta fario ) was selected as an indicator species. Enzymes first stage xenobiotic conversion, namely cytochrome P450 (CYP 450) ethoxyresorufin-O-deethylase (EROD) in liver were markers. Blood plasma vitellogenin concentrations evaluate xenoestrogenic effects...

Matriptase-2, a serine protease expressed in hepatocytes, is negative regulator of hepcidin expression. The purpose the study was to investigate interaction matriptase-2 with hemojuvelin protein vivo. Mice lacking proteolytic activity (mask mice) display decreased content protein. Vice versa, absence results liver matriptase-2, indicating that two proteins interact. To further characterize role we investigated iron metabolism mask mice fed experimental diets. Administration iron-enriched...

Hemojuvelin (HJV) is one of essential components for expression hepcidin, a hormone which regulates iron transport. HJV mainly expressed in muscle and liver, processing both tissues similar. However, hepcidin liver but not the role yet to be established. Our preliminary analyses mouse tissue showed that apparent molecular masses peptides are different (50 kDa monomer 35 20 heterodimer fragments) (55 34 possible large fragment heterodimer). One explanation glycosylation could lead difference...

Expression of hepcidin, the hormone regulating iron homeostasis, is increased by overload and decreased accelerated erythropoiesis or deficiency. The purpose study was to examine effect these stimuli, either alone in combination, on main signaling pathway controlling hepcidin biosynthesis liver, expression splenic modulators biosynthesis. Liver phosphorylated SMAD 1 5 proteins were determined immunoblotting male mice treated with dextran, kept an deficient diet, administered recombinant...