A5017751394- Gene Regulatory Network Analysis
- Evolution and Genetic Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Ecosystem dynamics and resilience
- Cellular transport and secretion
- Circadian rhythm and melatonin
- Protein Structure and Dynamics
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Microfluidic and Bio-sensing Technologies
- Diffusion and Search Dynamics
- Cell Image Analysis Techniques
- Origins and Evolution of Life
- Advanced Fluorescence Microscopy Techniques
- Molecular Communication and Nanonetworks
- Plant and Biological Electrophysiology Studies
- Bacterial Genetics and Biotechnology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Building Energy and Comfort Optimization
- Slime Mold and Myxomycetes Research
- Cellular Mechanics and Interactions
- Integrated Energy Systems Optimization
- Chaos, Complexity, and Education
- Bacterial biofilms and quorum sensing
Purdue University West Lafayette
2018-2024
Monash University
2023-2024
Australian Regenerative Medicine Institute
2024
University of Chicago
1996-2015
Chicago Institute for Psychoanalysis
2013
Vanderbilt University
2008
Fermi National Accelerator Laboratory
1996
Uncovering the quantitative laws that govern growth and division of single cells remains a major challenge. Using unique combination technologies yields unprecedented statistical precision, we find sizes individual Caulobacter crescentus increase exponentially in time. We also establish they divide upon reaching critical multiple ($\approx$1.8) their initial sizes, rather than an absolute size. show when temperature is varied, timescales scale proportionally with each other over...
Abstract Study Objectives: The nematode C. elegans develops through four larval stages before it reaches adulthood. At the transition between and sheds its cuticle, exhibits a sleep-like behavior during stage termed lethargus. objectives of this study were to characterize in detail behavioral patterns physiological activity command interneuron Measurements Results: We found that lethargus was composed bouts quiescence motion. duration individual ranged from 2 100 seconds, their dynamics...
How are granular details of stochastic growth and division individual cells reflected in smooth deterministic population numbers? We provide an integrated, multiscale perspective microbial dynamics by formulating a data-validated theoretical framework that accounts for observables at both single-cell scales. derive exact analytical complete time-dependent solutions to cell-age distributions rates as functionals the underlying interdivision time distributions, symmetric asymmetric cell...
Abstract Endosomal maturation is critical for robust and timely cargo transport to specific cellular compartments. The most prominent model of early endosomal involves a phosphoinositide-driven gain or loss proteins on individual endosomes, emphasising an autonomous stochastic description. However, limitations in fast, volumetric imaging long hindered direct whole cell-level measurements absolute numbers events. Here, we use lattice light-sheet bespoke automated analysis track very...
To survive dynamic environments, it is essential for all animals to appropriately modulate their behavior in response various stimulus intensities. For instance, the nematode Caenorhabditis elegans suppresses rate of egg-laying intense mechanical stimuli, a manner dependent on mechanosensory neurons FLP and PVD. We have found that unilaterally placed single interneuron ALA acted as high-threshold mechanosensor, was required this protective behavioral response.ALA inhibition strong...
Microfluidic platforms enable long-term quantification of stochastic behaviors individual bacterial cells under precisely controlled growth conditions. Yet, quantitative comparisons physiological parameters and cell different microorganisms in experimental device modalities is not available due to experiment-specific details affecting physiology. To rigorously assess the effects mechanical confinement, we designed, engineered, performed side-by-side experiments otherwise identical conditions...
Despite their simplicity, longitudinal studies of invertebrate models are rare. We thus sought to characterize behavioral trends Caenorhabditis elegans, from the mid fourth larval stage through young adult stage. found that, outside lethargus, animals exhibited abrupt switching between two distinct states: active wakefulness and quiet wakefulness. The durations epochs non-Poisson statistics. Increased Gαs signaling stabilized state before, during after lethargus. In contrast, decreased...
How do complex systems maintain key emergent “state variables” at desired target values to within specified tolerances? This question was first posed in the context of homeostasis living over a century ago, and yet precise quantitative rules governing this phenomenon have remained fiercely debated. We herein present direct solution through synthesis high-precision experiments principles-based physics theory. After introducing general approach that incorporates inherently stochastic dynamic...
We investigate the intergenerational shape dynamics of single Caulobacter crescentus cells using a novel combination imaging techniques and theoretical modeling. determine cell pole-to-pole lengths, cross-sectional widths, medial curvatures from high accuracy measurements contours. Moreover, these parameters are determined for over 250 across approximately 10000 total generations, which affords statistical precision. Our data model show that constriction is initiated early in cycle its...
Summary We report a novel method for obtaining simultaneous images from multiple vantage points of microscopic specimen using size‐matched mirrors created anisotropically etched silicon. The resulting pyramidal wells enable bright‐field and fluorescent side‐view images, when combined with z ‐sectioning, provide additional information 3D reconstructions the specimen. have demonstrated localization tracking over time centrosome live Dictyostelium discoideum. acquisition perspectives also...
Do individual bacterial cells retain memories of the history environmental conditions experienced in previous generations? Here we directly address this question through a synthesis physics theory and high-precision experiments on statistically identical, non-interacting cells, which grow divide with intrinsic stochasticity precisely controlled conditions. From these data, extract “emergent simplicities” seemingly complex interplay between dependence, persistence, transience stochastic...
Living systems are naturally complex and adaptive, offer unique insights into the strategies for achieving sustaining stochastic homeostasis in different conditions. Here, we focus on context of growth division individual bacterial cells. We take advantage high-precision longterm dynamical data that have recently been used to extract emergent simplicities articulate empirical intra- in-tergenerational scaling laws governing these dynamics. identify core motif mechanistic coupling between...
Organisms maintain the status quo, holding key physiological variables constant to within an acceptable tolerance, and yet adapt with precision plasticity dynamic changes in externalities. What organizational principles ensure such exquisite robust control of systems-level "state variables" complex systems extraordinary number moving parts fluctuating variables? Here we focus on these issues specific context intra- intergenerational life histories individual bacterial cells, whose...
Living systems are naturally complex and adaptive offer unique insights into the strategies for achieving sustaining stochastic homeostasis in different conditions. Here we focus on context of growth division individual bacterial cells. We take advantage high-precision long-term dynamical data that have recently been used to extract emergent simplicities articulate empirical intra- intergenerational scaling laws governing these dynamics. From data, identify core motif mechanistic coupling...
What are the signatures of onset catastrophe? Here we present rich system physics characterizing stochasticity driven transition from homeostasis to breakdown in an experimentally motivated and analytically tractable minimal model. Recent high-precision experiments on individual bacterial cells, growing dividing repeatedly a variety environments, have revealed previously unknown intergenerational scaling law which not only uniquely determines stochastic map governing homeostasis, but also,...
Organisms maintain the status quo, holding key physiological variables constant to within an acceptable tolerance, and yet adapt with precision plasticity dynamic changes in externalities. What organizational principles ensure such exquisite robust control of systems-level “state variables” complex systems extraordinary number moving parts fluctuating variables? Here, we focus on these issues specific context intra- intergenerational life histories individual bacterial cells, whose...
Endosomal maturation is critical for robust and timely cargo transport to specific cellular compartments. The most prominent model of early endosomal involves phosphoinositide-driven gain or loss proteins on individual endosomes, emphasising an autonomous stochastic description. However, limitations in fast, volumetric imaging long hindered direct whole-cell measurements absolute numbers events. Here, we use lattice light-sheet bespoke automated analysis track very (APPL1-positive)...
The interior of a living cell is an active, fluctuating, and crowded environment. Yet, it maintains high level coherent organization, which readily apparent in the intracellular transport network. Membrane-bound compartments called endosomes play key role carrying cargo, conjunction with myriad components including cargo adaptor proteins, membrane sculptors, motor cytoskeleton. These coordinate to effectively navigate specific locations, even though underlying protein interactions enzymatic...
Microfluidic platforms enable long-term quantification of stochastic behaviors individual bacterial cells under precisely controlled growth conditions. Yet, quantitative comparisons physiological parameters and cell different microorganisms in experimental device modalities is not readily possible owing to experiment-specific details affecting physiology confounding ways. To rigorously assess the effects mechanical confinement, we designed, engineered, performed side-by-side experiments...