A5087506470- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- Genetic factors in colorectal cancer
- Advanced biosensing and bioanalysis techniques
- Antifungal resistance and susceptibility
- MicroRNA in disease regulation
- Complement system in diseases
- Prenatal Screening and Diagnostics
- Renal and related cancers
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Lung Cancer Diagnosis and Treatment
- Fungal Infections and Studies
- Clinical Laboratory Practices and Quality Control
- Glycosylation and Glycoproteins Research
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- Bacteriophages and microbial interactions
- Pancreatic and Hepatic Oncology Research
- Ferroptosis and cancer prognosis
- Bacterial Identification and Susceptibility Testing
DRK Kliniken Berlin
2018-2024
University Hospital in Halle
2012-2019
Martin Luther University Halle-Wittenberg
2014-2018
Charité - Universitätsmedizin Berlin
2000-2011
Humboldt-Universität zu Berlin
1997-2011
Clinica Universidad de Navarra
2010
Praxis für Hämatologie und Onkologie
2005
Sana Klinikum Lichtenberg
2005
Deutsche Gesellschaft für Hämatologie und Medizinische Onkologie
2004
Cedars-Sinai Medical Center
1994-1995
Abstract Background This study aimed to show that SHOX2 DNA methylation is a tumor marker in patients with suspected lung cancer by using bronchial fluid aspirated during bronchoscopy. Such biomarker would be clinically valuable, especially when, following the first bronchoscopy, final diagnosis cannot established histology or cytology. A test low false positive rate can reduce need for further invasive and costly procedures ensure early treatment. Methods Marker discovery was carried out...
Highlights•Patients with cirrhosis are at high risk of hepatocellular carcinoma (HCC).•Accurate tumor biomarkers for the diagnosis and early detection HCC need to be developed.•The circulating, cell-free, epigenetic biomarker mSEPT9 is a promising diagnosing in patients cirrhosis.Patients (HCC). Several circulating markers under evaluation HCC, notably those identified through "omics" approaches. However, date, no has been shown useful individual patient level. Through initial replication...
Abstract The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE) was originally established in 2013, with main aims (i) promoting importance quality preanalytical phase testing process, (ii) establishing best practices providing guidance critical activities phase, (iii) developing disseminating surveys exploring concerning issues, (iv) organizing meetings, workshops, webinars or specific training courses on issues. As...
Production of E,E-farnesol (FOH) and biofilm formation were studied under various conditions in 56 strains eight Candida spp. FOH production differed significantly not only between but within albicans as well. concentrations the highest for C. albicans.
Abstract Background DNA methylation in the SHOX2 locus was previously used to reliably detect lung cancer a group of critical controls, including 'cytologically negative' samples with no visible tumor cell content, at high specificity based on analysis bronchial lavage samples. This study aimed investigate, if correlates gene expression and/or copy number alterations. An amplification together observed tumor-specific hypermethylation might explain good performance this marker Methods...
Purpose Most patients suffering from advanced lung cancer die within a few months. To exploit new therapy regimens we need better methods for the assessment of response. Material and Methods In pilot study prospectively enrolled 36 with NSCLC SCLC (34 stage IV, 2 IIIB) whom 34 received standard platinum-based chemo/radiotherapy two were treated tyrosine kinase inhibitor. We measured levels extracellular methylated SHOX2 DNA (mSHOX2) in plasma before during until re-staging. The mSHOX2...
Recently, in addition to the detection of circulating tumor cells peripheral blood patients with solid tumors, presence free nucleic acids plasma and serum has also been described. We have focused on possibility isolating amplifying intact extracellular, tumor-related mRNA from plasma/serum lung cancer. For this purpose, we established several RT-PCR-based amplification systems for a panel five different genes. The expression these genes was either shown be restricted tissue or associated...
In several papers an increased quantity of cell‐free plasma DNA as well the presence long fragments in and serum has been described. We isolated from plasma, serum, bronchial lavage supernatants 33 lung cancer patients 27 with a benign disease. The was amplified by real‐time PCR, integrity determined. did not find significant differences between patient populations. Our results led us to conclude that this method is useful diagnostic setting able differentiate
Cell‐free DNA is of increasing interest in different fields, such as cancer research, prenatal diagnosis, and the diagnosis nonmalignant diseases. The quantity free‐circulating plasma, serum, other body fluids usually low its isolation still a challenge. Here we evaluate application new commercially available NucleoSpin Plasma XS Kit for cell‐free from bronchial lavage samples. columns proved superior to QIAamp system terms yield, purity, retrieval small fragments. procedure fast easy...
Molecular markers in bronchial fluids may contribute to the diagnosis of lung cancer. We previously observed a significant increase C4d-containing complement degradation fragments bronchoalveolar lavage (BAL) supernatants from cancer patients cohort 50 cases and 22 controls (CUN cohort). The present study was designed determine diagnostic performance these (hereinafter jointly referred as C4d) fluids. C4d levels were determined BAL two independent cohorts: CU (25 26 controls) HUVR (60 98...
A bstract : In this paper, we show that the same panel of three microsatellite markers is useful for detection alterations in DNA tumor cells and plasma from patients diagnosed with SCLC NSCLC. 31% patients, detected a alteration(s) or LOH at least one locus. group NSCLC, alteration was locus 33% patients. all but 2 identical observed also isolated blood plasma. This work confirms results described by other groups it extends diagnostic possibilities finding cell‐specific freely circulating...
Abstract Background: Cytologic examination of specimens obtained from the respiratory tract is a lung cancer diagnostic procedure with high specificity but moderate sensitivity. The use molecular biomarkers may enhance sensitivity cytologic in detection cancer. Methods: Complement factor H, protein secreted by cells, was quantified series bronchoalveolar lavage supernatants patients and nonmalignant diseases. Albumin, total content, hemoglobin were also analyzed. Results validated...
Abstract It is well documented that in the chain from sample to result a clinical laboratory, pre-analytical phase weakest and most vulnerable link. This also holds for use analysis of extracellular nucleic acids. In this short review, we will summarize critically evaluate important steps phase, i.e. choice best control population patients be analyzed, actual blood draw, tubes drawing, impact delayed processing samples, method getting rid cells debris, matrix, plasma vs. serum other body...