14-3-3 proteins are found in all eukaryotes where they act as regulators of diverse signalling pathways associated with a wide range biological processes. In this study the functional characterization ZmGF14-6 gene encoding maize protein is reported. Gene expression analyses indicated that up-regulated by fungal infection and salt treatment plants, whereas its down-regulated drought stress. It reported rice plants constitutively expressing displayed enhanced tolerance to stress which was...

Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against broad spectrum of pathogens, thus having great biotechnological potential. Here, we report system for producing bioactive cecropin in rice seeds.Transgenic plants expressing codon-optimized synthetic gene drived by an endosperm-specific promoter, either the glutelin B1 or B4 were generated. The signal sequence from N-terminally fused to coding A. We also studied whether presence...

Abstract Background The Biopeptide BP100 is a synthetic and strongly cationic α-helical undecapeptide with high, specific antibacterial activity against economically important plant-pathogenic bacteria, very low toxicity. It was selected from library of peptides, along other peptides activities relevant bacterial fungal species. Expression the series in plants major interest to establish disease-resistant facilitate molecular farming. Specific challenges were small length, peptide...

Production of antimicrobial peptides in plants constitutes an approach for obtaining them high amounts. However, their heterologous expression a practical and efficient manner demands some structural requirements such as minimum size, the incorporation retention signals to assure accumulation specific tissues, presence protease cleavage amino acids target sequences facilitate peptide detection. Since any sequence modification may influence biological activity, that will be obtained from must...

A collection of 36 lipopeptides were designed from the cecropin A-melittin hybrid peptide BP100 (H-Lys-Lys-Leu-Phe-Lys-Lys-Ile-Leu-Lys-Tyr-Leu-NH2) previously described with activity against phytopathogenic bacteria. These synthesized on solid-phase and screened for their antimicrobial activity, toxicity proteolytic stability. They incorporated a butanoyl, hexanoyl or lauroyl group at N-terminus side chain lysine residue placed each position sequence. Their hemolysis depended fatty acid...

The hybrid peptide BP178 (KKLFKKILKYLAGPAGIGKFLHSAKKDEL-OH), derived from BP100 (KKLFKKILKYL) and magainin (1-10), engineered for plant expression, had a strong bactericidal activity but not fungicidal. Moreover, the preventive spray of tomato plants with controlled infections by pathogenic bacteria Pseudomonas syringae pv. Xanthomonas campestris vesicatoria, as well fungus Botrytis cinerea. treatment induced expression several genes according to microarray RT-qPCR analysis. Upregulated...

Cecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against broad spectrum of pathogens neoplastic cells, has important biotechnological applications. However, cecropin exploitation, as for other peptides, limited by their production purification costs. Here, we report the efficient this bioactive in rice bran using oleosin 18 carrier protein. High levels were reached seeds driving expression chimeric gene strong embryo-specific own promoter, targeting to oil...

Abstract Background Xylella fastidiosa is one of the most harmful bacterial plant pathogens worldwide, causing a variety diseases, with huge economic impact to agriculture and environment. Although it has been extensively studied, there are no therapeutic solutions suppress disease development in infected plants. In this context, antimicrobial peptides represent promising alternatives traditional compounds due their activity against wide range pathogens, low cytotoxicity, mode action that...

Protease inhibitors are biomolecules with growing biotechnological and biomedical relevance, including those derived from plants. This study investigated strong trypsin in quinoa, amaranth, lupine seeds, plant grains traditionally used Andean South America. Amaranth seeds displayed the highest inhibitory activity, despite having lowest content of aqueous soluble thermostable protein material. directly identified by enzymatic assay, HPLC, intensity-fading mass spectrometry (IF-MS), MS/MS, was...

BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering great potential for future applications in protection and other fields. Here we report the production recovery of bioactive using rice seeds as biofactories. A gene encoding was prepared introduced plants. The efficiently expressed transgenic under control an endosperm-specific promoter. Among three promoters (Glutelin B1, Glutelin B4 or Globulin 1), best...

is a plant pathogenic bacterium that has been introduced in the European Union (EU), causing significant yield losses economically important Mediterranean crops. Almond leaf scorch (ALS) currently one of most relevant diseases observed Spain, and no cure found to be effective for this disease. In previous reports, peptide BP178 shown strong bactericidal activity vitro against

Plant elicitor peptides (Peps) are endogenous molecules that induce and amplify the first line of inducible plant defense, known as pattern-triggered immunity, contributing to protect plants against attack by bacteria, fungi herbivores. Pep topic application transgenic expression have been found enhance disease resistance in a small number model plant-pathogen systems. The action Peps relies on perception specific receptors, so displaying family-specific activity. Recently, presence activity...

The kinetics of cell inactivation and the susceptibility Xylella fastidiosa subspecies fastidiosa, multiplex, pauca to synthetic antimicrobial peptides from two libraries (CECMEL11 CYCLO10) were studied. bactericidal effect was dependent on relative concentrations peptide bacterial cells, influenced by diluent, either buffer or sap. most lytic BP178, an enlarged derivative amphipathic cationic linear undecapeptide BP100. maximum reduction in survivors after BP178 treatment occurred within...

Transient expression of genes encoding peptides BP134 and BP178 by means a Potato virus X (PVX) based-vector system, treatment with synthetic endotherapy, were evaluated in the control Xylella fastidiosa infections, model plant Nicotiana benthamiana. production significantly decreased disease severity compared to PVX non-treated (NTC) plants, without adverse effects. Plants treated showed consistently lower levels than NTC plants. However, coinfection PVX-BP134 X. caused detrimental effects...

The activity of Eucalyptus essential oil against eleven strains pertaining to six species plant pathogenic bacteria was studied using growth inhibition and contact assays. All were susceptible the formulation EGL2, Xylella fastidiosa subspecies Xanthomonas fragariae most sensitive. bactericidal effect strong causing 4.5 6.0 log reductions in survival 30 min at concentrations range 0.75 15.0 μl/ml depending on tested. Transmission electron microscopy EGL2 three X. allowed observation a lytic...

The aggressiveness of Spanish isolates Xylella fastidiosa, representing different sequence types, were studied in almond plants several cultivars by means the dynamics population levels and symptoms, colonization spread, dose-effect relationships. Pathogen under greenhouse conditions showed doubling times 2.1 to 2.5 days during exponential growth phase, with a maximum size about 35 postinoculation (dpi). Differences patterns observed between sap xylem tissue after growth, as remained stable...

Protein protease inhibitors are biomolecules with growing biological, biotechnological and biomedical relevance, including those derived from plants. In this work, we compared by Intensity-Fading.Mass Spectrometry (IF-MS) other approaches the occurrence of strong protein against trypsin-type enzymes in seeds plants quinoa, amaranth lupine traditionally used South America. We differentially found that although have smaller quantity aqueous soluble thermostable material, they show highest...