A5059389860- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Extracellular vesicles in disease
- Anodic Oxide Films and Nanostructures
- Advanced biosensing and bioanalysis techniques
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- RNA Interference and Gene Delivery
- Studies on Chitinases and Chitosanases
- HIV-related health complications and treatments
- Thermal properties of materials
- Antimicrobial Peptides and Activities
- Molecular Biology Techniques and Applications
Waseda University
2019-2023
Exosomes transfer molecules horizontally to surrounding cells and therefore have a key role in cancer progression. To clarify the of exosomes progression, trace amounts proteins their lumen membrane fractions should be analyzed separately. For this purpose, an adequate easy-to-use method separating must developed. Further, because contain only proteins, ultrasensitive protein detection is necessary. develop fraction separation method, we applied commercially available kit originally...
Cancer cells communicate with each other via exosomes in the tumor microenvironment. However, measuring trace amounts of proteins is difficult, and thus cancer stemness-promoting mechanisms exosomal have not been elucidated. In present study, we attempted to quantify 78-kDa glucose-regulated protein (GRP78), which involved progression, released from cultured gastric using an ultrasensitive ELISA combined thio-NAD cycling. We also evaluated effects by application high-GRP78-containing cells....
For the diagnosis of disease, ability to quantitatively detect trace amounts causal proteins from bacteria/viruses as biomarkers in patient specimens is highly desirable. Here we introduce a simple, rapid, and colorimetric assay de novo, ultrasensitive detection method. This consists sandwich enzyme-linked immunosorbent (ELISA) thionicotinamide-adenine dinucleotide (thio-NAD) cycling, forming an ELISA, which signal substrate (i.e., thio-NADH) accumulates triangular manner, accumulated...
An enzyme immunoassay, in which an (e.g., alkaline phosphatase, ALP) is conjugated with antibody, a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive detection. The application phosphorylated substrate to ALP, followed by using dephosphorylated thionicotinamide-adenine dinucleotide cycling, provides quantification ALP. We describe protocol detecting ALP the zeptomole level colorimetric
We used single-enzyme cycling with 3α-hydroxysteroid dehydrogenase (3α-HSD) [9].The 3α-HSD catalyzes the