A5047327490- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Machine Learning in Bioinformatics
- Essential Oils and Antimicrobial Activity
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Plant-Microbe Interactions and Immunity
- Fibroblast Growth Factor Research
- Medicinal Plants and Neuroprotection
- Phytochemicals and Antioxidant Activities
- Lung Cancer Research Studies
- Bacterial Identification and Susceptibility Testing
- Tannin, Tannase and Anticancer Activities
- Cassava research and cyanide
- Animal Virus Infections Studies
- Genomics and Phylogenetic Studies
- Pharmacological Effects of Natural Compounds
- Weed Control and Herbicide Applications
- RNA Research and Splicing
- Mycorrhizal Fungi and Plant Interactions
- Phytochemistry and biological activity of medicinal plants
- Advanced NMR Techniques and Applications
- Lung Cancer Diagnosis and Treatment
- Plant and fungal interactions
University of Peshawar
2015-2024
Iqra National University
2024
Abstract Novel methods are required to enhance lung cancer detection, which has overtaken other cancer-related causes of death as the major cause mortality. Radiologists have long-standing for locating nodules in patients with cancer, such computed tomography (CT) scans. must manually review a significant amount CT scan pictures, makes process time-consuming and prone human error. Computer-aided diagnosis (CAD) systems been created help radiologists their evaluations order overcome these...
The fibroblast growth factor receptor 3 (FGFR3) is warranted as a promising therapeutic target in bladder cancer it described 75% of papillary tumors. Considering this, the present study was conducted to use different approaches computer-aided drug discovery (CADD) identify best binding compounds against active pocket FGFR3. Compared control pyrimidine derivative, identified three lead structures; BDC_24037121, BDC_21200852, and BDC_21206757 with energy value −14.80 kcal/mol, −12.22 −11.67...
Enhancers are sequences with short motifs that exhibit high positional variability and free scattering properties. Identification of these noncoding DNA fragments their strength extremely important because they play a key role in controlling gene regulation on cellular basis. The identification enhancers is more complex than other factors the genome freely scattered, location varies widely. In recent years, bioinformatics tools have enabled significant improvement identifying this biological...
Abstract Problem Background Penicillin was the first and most famous fungal secondary metabolite used as broad spectrum antibiotic that revolutionarised pharmaceutical research also saved millions of lives. The over optimistic belief in 1967 sufficient antibiotics had been discovered to defeat infectious diseases quickly crashed with appearance multidrug resistant (MDR) bacteria 1990s. This has posed a serious threat mankind. Although scientists are making efforts synthesize discover new...
Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1% growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion assays demonstrated that crude extract obtained from GPYB showed maximum zone (8.8 0.4 mm) pathogen, which is corroborated by comparative LCMS profiles extracts all three media i.e. GPYB, YEB PDB. Two known...
Following preliminary bioactivity testing, the fungal strain identified as Penicillium crysogenum was cultured in a modified Czapec Yeast Broth medium (CYB) for production of antifungal compounds. Several chromatographic techniques including HPLC were used to purify metabolites from crude extract. The mass determination purified compound performed using Water's LCMS system while structure elucidated 400 and 500 Varian NMR machines. chemical name is (2 R, 4S) -2, 4-dimethyl-4-((E)-2-((3S,...
Background: Peptidases are a group of enzymes which catalyze the cleavage peptide bonds. Around 2-3% whole genome codes for proteases and about one-third all known serine divided into 13 clans 40 families. They involved in diverse physiological roles such as digestion, coagulation blood, fibrinolysis, processing proteins prohormones, signaling pathways, complement fixation, have vital role immune defense system. Based on their functions, they can broadly be two classes; GASPIDs (Granule...
To investigate the prevalence of Staphylococcus aureus (S. aureus) isolated from skin wards hospitals Khyber Pakhtunkhwa, its resistance against various commonly and commercially available antibiotics, as well different genetic traits their correlations with phenotypic visible resistance. In present study a simple PCR technique were used to in S. two major Pakistan. A total 100 samples collected both male female, which 50 patient's site infection ward environment. These results demonstrated...
The discovery of bioactive compounds from fungal natural sources holds immense potential for the development novel therapeutics. present study investigates extracts soil-borne Penicillium notatum and rhizosphere-inhabiting Aspergillus flavus their antibacterial, antifungal, cytotoxic potential. Additionally, two were purified using chromatographic spectroscopic techniques. results demonstrated that ethyl acetate fraction A. exhibited prominent activity against Artemia salina, whereas P....
<title>Abstract</title> The rise of microbial resistance and emerging infections pose significant health threats. Natural products from endophytic fungi offer a promising source novel compounds with potential as major drug leads. This research aims to screen <italic>Myrtus communis</italic> <italic>Moringa oleifera</italic> for their metabolites antibacterial antifungal potential. Six fungal strains were isolated using Potato Dextrose Agar (PDA) medium. <italic>M. isolates designated MC1,...
Background: The emergence of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, notably Omicron and Delta, necessitates a detailed analysis the amino acid substitutions occurring within spike (S) protein. mentioned above are crucial in regulating interactions between virus its host receptor angiotensin-converting enzyme (ACE2), addition to impacting effectiveness monoclonal antibodies (mAbs) used therapy. Methods: In this paper, we employed computational mutational...
Bioassay-guided isolation of a dihydroxy flavone from the cell extract Penicillium EU0013 was carried out using preparative LCMS and structure interpreted 1D 2D NMR spectra. The fungus fermented in three different nutrient media i.e. Glucose Nutrient Broth, Peptone Yeast Extract Broth obtained organics. Comparative profiles confirmed enhanced production GPYB media. In this study, pathogenicity is reported for first time against Fusarium oxysporum f.sp. lycopersici, wilt causing pathogen...
In the quest for bioactive natural products of fungal origin, Aspergillus flavus was isolated from rhizosphere Mentha piperita using Potato Dextrose Agar (PDA) and Czapec Yeast Broth (CYB) nutrient media metabolites production. total, three different were purified HPLC/LCMS structures established 500 Varian NMR experiments. Further in concentrations (10, 100, 1000 μg/mL) tested herbicidal activity Completely Randomized design (CRD) against seeds Silybum marianum Avena fatua which are major...
The recent emergence of a novel coronavirus strain (SARS-CoV-2) has stimulated global efforts to identify potential drugs that target proteins expressed by this coronavirus. Among these, the main protease SARS-CoV-2 (3CL-protease (3CLPro), also known as (MPro) is one best choices for scientists target. 3CLPro involved in processing polyproteins into mature non-structural viral proteins. An X-ray crystallographic structure (PDB ID 6LU7) protein was obtained from PDB database. ChemDiv...