A5067233127- Muscle Physiology and Disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Diabetes and associated disorders
- Genetic Neurodegenerative Diseases
- Ubiquitin and proteasome pathways
- Adipose Tissue and Metabolism
- Cardiac Ischemia and Reperfusion
- Natural product bioactivities and synthesis
- Cardiac Arrest and Resuscitation
- Plant biochemistry and biosynthesis
- Phytochemicals and Antioxidant Activities
- Mechanical Circulatory Support Devices
- Autophagy in Disease and Therapy
University of Birmingham
2020-2025
Versus Arthritis
2021-2024
The Royal Melbourne Hospital
1995-2012
Abstract Transcriptionally mature and immature β-cells co-exist within the adult islet. How such diversity contributes to insulin release remains poorly understood. Here we show that subtle differences in β-cell maturity, defined using PDX1 MAFA expression, contribute islet operation. Functional mapping of rodent human islets containing proportionally more HIGH reveals defects metabolism, ionic fluxes secretion. At transcriptomic level, presence increased numbers leads dysregulation gene...
MuRF1 (Muscle-specific RING finger protein 1; gene name TRIM63) is a ubiquitin E3 ligase, associated with the progression of muscle atrophy. As (Really Interesting New Gene) type its unique activity ubiquitylation driven by specific interaction UBE2 (ubiquitin conjugating enzyme). Our understanding function remains unclear as candidate UBE2s have not been fully elucidated. In present study, we screened human dependent in vitro and found that engages UBE2D, UBE2E, UBE2N/V families UBE2W. can...
MuRF1 [muscle RING (Really Interesting New Gene)-finger protein-1] is an ubiquitin-protein ligase (E3), which encode by TRIM63 (tripartite motif containing 63) gene, playing a crucial role in regulating cardiac muscle size and function through ubiquitylation. Among hypertrophic cardiomyopathy (HCM) patients, 24 variants have been identified, with 1 additional variant linked to restrictive cardiomyopathy. However, only three previously investigated for their functional effects. The structural...
Atrogin-1 and Muscle-specific RING finger protein 1 (MuRF1) are highly expressed in multiple conditions of skeletal muscle atrophy. The phosphoinositide 3-kinase (PI3K)/Akt/forkhead box (FoxO) signaling pathway is well known to regulate MuRF1 gene expressions. However, Akt activation also activates the mechanistic target rapamycin complex (mTORC1), which induces hypertrophy. Whether mTORC1-dependent has a role regulating and/or expression currently unclear. In this study, we showed that...
L-arginine cardioplegia decreases biochemical markers of myocardial damage and oxidative stress in patients with normal left ventricular function. We investigated the effects supplemented cardioplegic arrest reduced ejection fraction. Fifty-three adult fraction <35% undergoing elective coronary artery bypass surgery were randomised to receive blood or without L-arginine. Following cardiopulmonary bypass, measured endpoints cardiac troponin-I concentration at 12 24 hours, sinus...
Calamus caesius is known as Rattan. It was found a component of many formulas from evidence-based Thai medicinal scriptures but no research about their properties. We investigated the literature review analysis textbooks for proposed biological activity relatives including antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities, chemical profiles using gas chromatography-mass spectrometry (GC–MS). In silico studies were inspected on tyrosinase NAD(P)H oxidase actions. Thirty...
Abstract: Xenografts of organ cultured fetal pig pancreas in prediabetic NOD mice can survive for prolonged periods (>20 weeks) recipients treated with anti‐T cell monoclonal antibodies (MAb) directed against host CD4 and CD3 surface molecules. Anti‐CD4 MAb treatment alone is only partly effective xenograft rejection occurs over a period many weeks. In diabetic recipients, by contrast, recurrence autoimmune disease isografts rapid (<28 days) despite similar depletion CD4+ve T cells....
Abstract Transcriptionally mature and immature β-cells co-exist within the adult islet. How such diversity contributes to insulin release remains poorly understood. Here we show that differences in β-cell maturity, defined using PDX1 MAFA expression, are required for proper islet operation. Functional mapping of rodent human islets containing proportionally more revealed defects metabolism, ionic fluxes secretion. At transcriptomic level, presence increased numbers led dysregulation gene...
Mice deficient in the respiratory chain gene Cox6a2
ABSTRACT MuRF1 (Muscle-specific RING finger protein 1; gene name TRIM63) is a ubiquitin E3 ligase, associated with the progression of muscle atrophy. As (Really Interesting New Gene)-type its unique activity ubiquitylation driven by specific interaction UBE2 (ubiquitin conjugating enzyme) to ubiquitylate substrate protein. The understanding function remains unclear as candidate has not been elucidated and thus mechanism inconclusive. In present study, we screened human dependent E2s using...
Abstract Muscle-specific ubiquitin E3 ligases, Atrogin-1 and MuRF1, are highly expressed in multiple conditions of skeletal muscle atrophy. The PI3K/Akt/FoxO signaling pathway is well known to regulate MuRF1 gene expressions. Evidence supporting this largely based on stimuli by insulin IGF-1, that activate anabolic signaling, including Akt Akt-dependent transcription factors. However, activation also activates the mammalian target rapamycin complex 1 (mTORC1) which induces hypertrophy....