A5033726539- Proteoglycans and glycosaminoglycans research
- Protease and Inhibitor Mechanisms
- Diagnosis and Treatment of Venous Diseases
- Venous Thromboembolism Diagnosis and Management
- Connective tissue disorders research
- Fibroblast Growth Factor Research
- Collagen: Extraction and Characterization
- Aortic aneurysm repair treatments
- Dermatologic Treatments and Research
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Pregnancy and preeclampsia studies
- Fatty Acid Research and Health
- Calpain Protease Function and Regulation
- Systemic Sclerosis and Related Diseases
- Metastasis and carcinoma case studies
- Polyamine Metabolism and Applications
- Blood Coagulation and Thrombosis Mechanisms
- Oral microbiology and periodontitis research
- Ubiquitin and proteasome pathways
- Uterine Myomas and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Sarcoma Diagnosis and Treatment
- Bone and Dental Protein Studies
- Lipoproteins and Cardiovascular Health
Medical University of Białystok
2009-2022
In this study, we report quantity, solubility and molecular polymorphism of collagen, proportional relationships between various types ultrastructure collagen fibres, the amounts glycosaminoglycans (GAGs) them in Wharton's jelly normal umbilical cords. We compare extracellular matrix components with those cord artery. Collagen demonstrates some specific features. It is very insoluble neutral salt a slightly acidic solution appears to be resistant action depolymerizing agent (EDTA-Na2). Only...
<i>Objective:</i> The defects of collagen metabolism are responsible for the disorganization extracellular matrix in stomach cancer. Collagen through interaction with integrin receptors regulates cellular growth, differentiation, gene expression, prolidase and gelatinase activity plays an important role tumorigenesis invasiveness. Although metalloproteinases initiate breakdown tissues, final step its degradation is mediated by prolidase. Therefore, we decided to compare control...
<i>Objective(s):</i> Extracellular matrix remodeling in the vein wall is involved varicose pathogenesis, with transforming growth factor β<sub>1</sub> (TGF-β<sub>1</sub>) playing a potential role. The aim of study was to assess TGF-β signaling pathway including its receptor (TGF-β RII) and phosphorylated receptor-regulated Smads (p-Smad2/3) veins. <i>Methods:</i> Varicose veins from patients undergoing surgery were studied material, whereas...
The amounts and activities of matrix metalloproteinases (MMPs) were studied in human myometrium uterine leiomyomas various stages growth. It was found that both the investigated tumors contain collagenolytic enzymes. MMP-1, MMP-2, MMP-3 MMP-9 found. Gelatinase A (MMP-2) is most abundant. In control only 10% this enzyme exists an active form, whereas tumors, especially large ones, values reach 30%. suggested high activity MMP-2 responsible for remodelling extracellular growing tumors.
Pre-eclampsia--edema, proteinuria, hypertension (EPH-gestosis) is one of the more common complications observed during pregnancy. The umbilical cord vein walls were taken from newborns delivered by healthy mothers (control material) and with polysymptomatic pre-eclampsia (investigated material). Normal saphenous collected adult subjects undergoing varicose surgery. collagen content was measured assay hydroxyproline. Elastin determined according to Fastin Assay gravimetrically....
Abstract There is a host of evidence for the role bioactive sphingolipids in cancer biology, and dysregulated sphingolipid metabolism was observed many malignant tumors. The aim present study to provide more detailed data on different stages clear cell renal carcinoma (ccRCC). Samples tumor noncancerous fragments same kidney were collected from patients who underwent radical nephrectomy. subjects stratified according degree malignancy ( n = 14 G2, 12 G3, 9 G4). content...
Our earlier paper has reported that Wharton's jelly is a reservoir of several peptide growth factors, including acidic and basic fibroblast factors (aFGF bFGF, respectively). Both can be extracted by buffered salts solutions in the form high molecular mass complexes, probably with component(s) extracellular matrix. aFGF bFGF from such extracts hardly penetrate 10% polyacrylamide gels during electrophoresis. Pre-treatment hyaluronidase slightly increased extractability aFGF, but did not...
The purpose of this study was to develop a new method for determination the cathepsin L—biosensor based on Surface Plasmon Resonance Imaging technique. L is an endopeptidase, which degrades proteins and plays important role in various processes occurring human body. detection technique, Imaging, optical, label-free can be used quantitative different proteins. In order bind enzyme, inhibitor—RKLLW-NH2 used. validation process showed that parameters: precision, accuracy, selectivity were...
The abdominal aortic aneurysm (AAA) wall represents an extreme example of arterial remodeling with disturbed elastin, collagen and proteoglycan metabolism. aim this study was to evaluate enzymes involved in the degradation glycosaminoglycan chains core proteins proteoglycans AAA wall. material consisted samples from 10 AAA. Fragments 5 normal aortas organ donors were used as a control. activity endoglycosidases, exoglycosidases sulfatases measured using colorimetric methods. To assess matrix...
The varicose vein wall remodeling is a very complex process, which controlled by numerous factors, including peptide growth factors. aim of the study was to assess a/b FGF, IGF-1, TGF-β1, VEGF-A and their receptors in wall. Varicose samples were taken from 24 patients undergoing surgery. control material consisted specimens collected 12 with chronic limb ischemia. Contents aFGF, bFGF, IGF-I, VEGF, IGF-1R, VEGF R1 R2 assessed ELISA method. Protein expression FGF TGF-β RII evaluated western...
The walls of human abdominal aortas and atherosclerosis-induced aneurysms contain similar amounts collagen. quantitative ratio between collagens various types this protein does not differ significantly either, whereas solubility the collagen in aneurysmal wall its susceptibility to action EDTA are distinctly decreased. In contrast with collagen, amount elastin is lower. Total glycosaminoglycans slightly decreased as compared that normal tissue, but particular compounds varies. percentage...
Extensive extracellular matrix remodeling of the vein wall is involved in varicose veins pathogenesis. This process controlled by numerous factors, including peptide growth factors. The aim study was to evaluate influence thrombophlebitis on TGF-β1 and its signaling pathway wall. mRNAlevels, factor content expression were evaluated RT-PCR, ELISA, western blot methods, respectively, walls normal veins, complicated thrombophlebitis. Western analysis used assess TGF-β receptor type II (TGF-β...
Abstract Insulin-like growth factor-I (IGF-I) is known as an important stimulator of collagen and glycosaminoglycans (GAGs) biosynthesis in tissues. IGF-I activity under control IGF-I-binding proteins (IGFBPs) with different affinity. IGFBP-1 inhibitor IGF-dependent functions. Some IGFBPs (e.g., IGFBP-1) may undergo phosphorylation that dramatically increases IGFBP affinity for IGF. Wharton's jelly represents a reservoir its binding (BPs). Pre-eclampsia, the most common, pregnancy-associated...
Human urinary bladder cancer is a huge worldwide oncological problem causing many deaths every year. The degradation of extracellular matrix (ECM) induced by molecules such as metalloproteinases (MMPs) one the main factors influencing process metastasis origination. MMP expression tied to tumor aggressiveness, stage, and patient prognosis. cleavage constituent proteins initiated prolonged metalloproteinases, MMP-3 MMP-10. aim this study was evaluate activity both MMPs in human occurring at...