A5085565336- Electrochemical sensors and biosensors
- Enzyme Production and Characterization
- Proteins in Food Systems
- Carbon and Quantum Dots Applications
- Electrochemical Analysis and Applications
- Building materials and conservation
- Tea Polyphenols and Effects
- Graphene and Nanomaterials Applications
- Conservation Techniques and Studies
- Advanced biosensing and bioanalysis techniques
- Fungal and yeast genetics research
- Monoclonal and Polyclonal Antibodies Research
- Identification and Quantification in Food
- Plant and fungal interactions
- Microbial bioremediation and biosurfactants
- Enzyme Catalysis and Immobilization
- Protist diversity and phylogeny
- Indoor Air Quality and Microbial Exposure
- Enzyme-mediated dye degradation
- Marine Toxins and Detection Methods
- RNA Interference and Gene Delivery
- Animal and Plant Science Education
- Cultural Heritage Materials Analysis
- Biotin and Related Studies
- Biochemical and Structural Characterization
University of Naples Federico II
2019-2024
Universidad de Oviedo
2023
Federico II University Hospital
2019-2021
For their easy and high-yield recombinant production, high stability in a wide range of physico-chemical conditions characteristic hollow structure, ferritins (Fts) are considered useful scaffolds to encapsulate bioactive molecules. Notably, for the absence immunogenicity selective interaction with tumor cells, nanocages constituted by heavy chain human variant ferritin (hHFt) optimal candidates delivery anti-cancer drugs. hHFt can be disassembled reassembled vitro allow loading cargo...
Marine microorganisms represent a reservoir of new promising secondary metabolites. Surface-active proteins with good emulsification activity can be isolated from fungal species that inhabit the marine environment and candidates for different biotechnological applications. In this study novel surface-active protein, named Sap-Pc, was purified strain Penicillium chrysogenum. The effect salt concentration temperature on protein production analyzed, purification method set up. identified as...
The unprecedented role of carbon nanotubes in promoting and sensing enzymatic oxidation polycyclic aromatic hydrocarbons by laccases enables femtomolar detection anthracene.
Identification and characterization of ancient proteins still require technical developments towards non-invasiveness, sensitivity, versatility ease use the analyses. We report that enzyme functionalized films, described in Cicatiello et al. (2018), can be used efficiently on surface different objects ranging from fixative-coated paper to canvas coating an albumen photograph, as well much harder surfaces ivory proteinaceous binders decoration a wooden Egyptian coffin. The mixture digested...
The immobilization of POXA1b laccase and its hydrophobin-fused chimera was performed at pristine Multiwalled Carbon Nanotube (MWCNT) MWCNT-modified electrodes by electrografting a 2-diazoniumanthraquinone salt. influence the hydrophobin domain MWCNT functionalization with anthraquinone groups towards compared direct electrochemistry under O2 electrochemical biosensing phenols. affords stable dispersion in water/ethanol, while being detrimental to electron transfer between electrode. On...
Fungi produce surface-active proteins, among which hydrophobins are the most characterized and attractive also for their ability to form functional amyloids. Our recent findings show that these abilities shared with other classes of fungal proteins. Indeed, in this paper, we compared characteristics a class I hydrophobin (Vmh2 from Pleurotus ostreatus) an unknown protein (named PAC3), extracted marine strain Acremonium sclerotigenum, does not belong same family based on its sequence...
A chimeric enzyme based on the genetic fusion of a laccase with hydrophobin domain was employed to functionalize few-layer graphene, previously exfoliated from graphite in presence hydrophobin. The as-produced, biofunctionalized graphene characterized by electrochemistry and Raman spectroscopy, finally biosensing phenols such as catechol dopamine. This strategy paves way for functionalization nanomaterials domains enzymes their use variety electrochemical applications.
Although antibody immobilization on solid surfaces is extensively used in several applications, including immunoassays, biosensors, and affinity chromatography, some issues are still challenging. Self-assembling protein layers can be to coat easily different by direct deposition. A specific biofunctional layer formed using genetic engineering techniques express fused proteins acting as self-immobilizing antibodies. In this study, fusion combining the self-assembling adhesive properties of a...
Performing on-site measurements is one of the main challenges current (bio)chemical analysis. Micropipette tips, which are among most common utilities in laboratories, can be useful and cheap elements for designing out-of-the-box innovative analytical biodevices. Furthermore, properties these bioplatforms depend on immobilization recognition element (e.g., enzymes). In this context, we have recently designed a chimeric protein laccase PoxA1b from fungus Pleurotus ostreatus has been...
Exposing artworks and artifacts in museums is fundamental to disseminate culture knowledge but also risky since the exposure environmental conditions can deteriorate exhibited objects. Light crucial, being able trigger photochemical effects, jointly responsible (with temperature humidity) for biological risk generation. In this context, fungal growth museum a damaging factor that should not be underestimated. Consequently, study of lighting effects on fungi development essential. work, five...
The use of multichannel LEDs is crucial in museum applications to design ad-hoc spectra able combine fruition and protection needs, i.e., maximizing Colour Rendering Index controlling energy content risk wavelengths responsible for the photochemical degradation exposed objects. A similar approach could be applied also prevent biological risk, such as fungi growth on artifacts, studying microorganisms’ sensitivity different wavelengths. paper investigates rate proteins carbohydrates five...
Performing on-site measurements is one of the main challenges current (bio)chemical analysis. Innovative approaches are continuously appearing to produce simple, low-cost, fast response and field-deployable devices. Micropipette tips, most common utilities in laboratories, can be useful supports for designing innovative They cheap materials widely employed many purposes, mainly liquid dispensing. Furthermore, development surface-based biodevices, immobilization recognition element a key...
Performing on-site measurements is one of the main challenges current (bio)chemical analysis. Micropipette tips, which are among most common utilities in laboratories, can be useful and cheap elements for designing innovative devices. They widely employed many purposes, liquid dispensing a required step. Furthermore, development surface-based biodevices, immobilization recognition element crucial; analytical properties, mainly selectivity sensitivity, depend on it. As regards enzyme...