A5040466285- Immune Response and Inflammation
- interferon and immune responses
- Influenza Virus Research Studies
- Infection Control and Ventilation
- SARS-CoV-2 detection and testing
- Rabies epidemiology and control
- Genomics and Rare Diseases
- Hemoglobinopathies and Related Disorders
- Microbial infections and disease research
- COVID-19 and healthcare impacts
- Genomics and Phylogenetic Studies
- Iron Metabolism and Disorders
University of Cambridge
2018-2024
University of Glasgow
2023
MRC University of Glasgow Centre for Virus Research
2023
Addenbrooke's Hospital
2018-2022
Royal Prince Alfred Hospital
2022
Influenza A viruses (IAV) and SARS-CoV-2 can spread via liquid droplets aerosols. Face masks other personal protective equipment (PPE) act as barriers that prevent the of these viruses. However, IAV are stable for hours on various materials, which makes frequent correct disposal PPE important. Metal ions embedded into may inactivate respiratory viruses, but confounding factors such adsorption make measuring optimizing inactivation characteristics difficult. Here, we used polyamide 6.6 (PA66)...
During infection, the influenza A virus RNA polymerase produces both full-length and aberrant molecules, such as defective viral genomes (DVGs) mini RNAs (mvRNAs). Subsequent innate immune activation involves binding of host pathogen receptor retinoic acid–inducible gene I (RIG-I) to RNAs. However, it is not clear what factors determine which are RIG-I agonists. Here, we provide evidence that structures, called template loops (t-loops), stall contribute by mvRNAs during infection. Impairment...
During influenza A virus (IAV) infection, host pathogen receptor retinoic acid-inducible gene I (RIG-I) detects the partially complementary, 5'-triphosphorylated ends of viral genome segments and non-canonical replication products. However, it has also been suggested that innate immune responses may be triggered by transcription. In this study, we investigated whether an immunostimulatory RNA is produced during IAV We show polymerase can read though polyadenylation signal transcription...
Genomic data can be used to track the transmission and geographic spread of infectious diseases. However, sequencing capacity required for genomic surveillance remains limited in many low- middle-income countries (LMICs), where dog-mediated rabies and/or transmitted by wildlife such as vampire bats pose major public health economic concerns. We present here a rapid affordable sample-to-sequence-to-interpretation workflow using nanopore technology. Protocols sample collection diagnosis are...
Infections with respiratory viruses can spread via liquid droplets and aerosols, cause diseases such as influenza COVID-19. Face masks other personal protective equipment (PPE) act barriers that prevent the of containing these viruses. However, A coronaviruses are stable for hours on various materials, which makes frequent correct disposal PPE important. Metal ions embedded into may inactivate viruses, but confounding factors absorption make measuring optimizing inactivation characteristics...
Influenza A virus infection usually causes a mild to moderately severe respiratory disease in humans. However, with the 1918 H1N1 pandemic or highly pathogenic avian influenza viruses (HPAIV) of H5N1 subtype, can lead viral pneumonia, systemic and death. The molecular processes that determine outcome are multifactorial involve complex interplay between host, viral, bacterial factors 1 . it is generally accepted strong innate immune dysregulation known as ‘cytokine storm’ contributes...
Abstract During infection, the influenza A virus RNA polymerase produces both full-length and aberrant molecules, such as defective viral genomes (DVG) mini RNAs (mvRNA). Subsequent innate immune activation involves binding of host pathogen receptor retinoic acid-inducible gene I (RIG-I) to RNAs. However, not all are strong RIG-I agonists. Here we show that potent by mvRNAs is determined transient structures, called template loops (t-loop) stall polymerase. The effect t-loops depends on...