A5018205874- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Calpain Protease Function and Regulation
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Cellular Mechanics and Interactions
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
Universität Hamburg
2019-2024
University Medical Center Hamburg-Eppendorf
2019-2024
Charité - Universitätsmedizin Berlin
2024
Einstein Center for Neurosciences Berlin
2024
Humboldt-Universität zu Berlin
2021-2023
ABSTRACT In neurons, the microtubule (MT) cytoskeleton forms basis for long-distance protein transport from cell body into and out of dendrites axons. To maintain neuronal polarity, axon initial segment (AIS) serves as a physical barrier, separating somatodendritic compartment acting filter axonal cargo. Selective trafficking is further instructed by enrichment MT post-translational modifications, which affect dynamics activity motor proteins. Here, we compared two knockout mouse lines...
In this study, we analyzed the functional role of formin Drosophila Homologue Diaphanous2 (Diaph2) in colorectal cancer cells. We show that stable down-regulation Diaph2 expression HT29 cells decreased chromosome alignment and velocity movement during M-phase, thus reducing proliferation rate colony formation. interphase cells, was diffusely distributed cytosol, while metaphase protein located to spindle microtubules (MTs). Diaph2-depletion increased concentration MTs, showing is required...
Detyrosination is a major post-translational modification of microtubules (MTs), which has significant impact on MT function in cell division, differentiation, growth, migration and intracellular trafficking. α-tubulin occurs mostly via the recently identified complex vasohibin 1 or 2 (VASH1 VASH2, respectively) with small binding protein (SVBP). However, there still remaining detyrosinating activity absence VASH1 and/or VASH2 SVBP, little known about regulation detyrosination. Here, we...
Abstract Detyrosination is a major post-translational modification of microtubules (MT), which has significant impact on MT function in cell division, differentiation, growth, migration, polarity, and intracellular trafficking. α-tubulin occurs via the recently identified complex vasohibin 1/2 (vash1/2) small binding protein (SVBP). However, there still remaining detyrosinating activity absence vash1/2/SVBP, little known about regulation detyrosination. Using cellular cell-free assays we...
Abstract In neurons, the microtubule (MT) cytoskeleton forms basis for long-distance protein transport from cell body into and out of dendrites axon. To maintain neuronal polarity, axon initial segment (AIS) serves as a physical barrier, separating somatodendritic compartment acting filter axonal cargo. Selective trafficking is further instructed by enrichment MT post-translational modifications, which affect dynamics activity motor proteins. Here, we compared two knockout mouse lines...