A5016660792- Marine Ecology and Invasive Species
- Planarian Biology and Electrostimulation
- bioluminescence and chemiluminescence research
- Marine Invertebrate Physiology and Ecology
University of Kentucky
2021-2025
The regulation of chromatin accessibility is essential in eukaryotic cells as one several mechanisms that ensure gene activation occurs at appropriate times and cell types. Accordingly, mutations remodeling proteins are linked to many different developmental disorders cancers. One example a protein has been both abnormalities cancer BPTF/NURF301, the largest subunit Nucleosome Remodeling Factor (NuRF) complex. BPTF not only important for formation NuRF but also helps direct its activity...
Trimethylation of histone H3 lysine 4 (H3K4me3) correlates strongly with gene expression in many different organisms, yet the question whether it plays a causal role transcriptional activity remains unresolved. Although H3K4me3 does not directly affect chromatin accessibility, can indirectly genome accessibility by recruiting ATP-dependent remodeling complex NuRF (Nucleosome Remodeling Factor). The largest subunit NuRF, BPTF/NURF301, binds specifically and recruits to loci marked this...
Tumor suppressor genes (TSGs) are essential for normal cellular function in multicellular organisms, but many TSGs and tumor-suppressing mechanisms remain unknown. Planarian flatworms exhibit particularly robust tumor suppression, yet the specific underlying this trait unclear. Here, we analyze histone H3 lysine 4 trimethylation (H3K4me3) signal across planarian genome to determine if broad H3K4me3 chromatin signature that marks cell identity mammalian cells is conserved valuable model of...
The overwhelming majority of RNA species isolated from cells or tissues using organic extraction are ribosomal RNAs (rRNA), whereas a relatively small percentage messenger (mRNA). For studies that seek to detect mRNA transcripts and measure changes in their expression, this lopsided ratio desired undesired creates significant challenge obtaining sensitive reproducible results. One method for improving detection is selectively amplify polyadenylated (polyA) molecules when generating RNA-seq...