A5080296988- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Legume Nitrogen Fixing Symbiosis
- Plant and animal studies
- Plant Parasitism and Resistance
- Prion Diseases and Protein Misfolding
- Genomics and Chromatin Dynamics
- HIV Research and Treatment
Massachusetts Institute of Technology
2021-2024
Whitehead Institute for Biomedical Research
2024
Wisconsin Institutes for Discovery
2020-2023
University of Wisconsin–Madison
2020-2023
Howard Hughes Medical Institute
2021-2023
McGovern Institute for Brain Research
2021-2023
Broad Institute
2021-2023
Prion disease is caused by misfolding of the prion protein (PrP) into pathogenic self-propagating conformations, leading to rapid-onset dementia and death. However, elimination endogenous PrP halts progression. In this study, we describe Coupled Histone tail for Autoinhibition Release Methyltransferase (CHARM), a compact, enzyme-free epigenetic editor capable silencing transcription through programmable DNA methylation. Using histone H3 tail-Dnmt3l fusion, CHARM recruits activates...
To spread, transposons must integrate into target sites without disruption of essential genes while avoiding host defense systems. Tn7-like employ multiple mechanisms for target-site selection, including protein-guided targeting and, in CRISPR-associated (CASTs), RNA-guided targeting. Combining phylogenomic and structural analyses, we conducted a broad survey selectors, revealing diverse used by Tn7 to recognize sites, previously uncharacterized target-selector proteins found newly...
Histone 3 lysine 79 methylation (H3K79me) is enriched on gene bodies proportional to expression levels and serves as a strong barrier for the reprogramming of somatic cells induced pluripotent stem (iPSCs). DOT1L sole histone methyltransferase that deposits all three orders—mono (me1), di (me2), tri (me3) methylation—at H3K79. Here, we leverage genetic chemical approaches parse specific functions orders H3K79me in maintaining cell identity. interacts with AF10 (Mllt10), which recognizes...
Abstract Halogenation chemistry is rare in plant metabolism, with the chloroalkaloid acutumine produced by Menispermaceae species being only well characterized example, involving a specialized dechloroacutumine halogenase (DAH) from iron(II)- and 2-oxoglutarate-dependent dioxygenase (2ODD) superfamily. While DAH presumed to have evolved an ancestral 2ODD enzyme, broader question of how new enzymes arise through Darwinian processes, such as birth Menispermaceae, remains fundamental challenge...
ABSTRACT Histone 3 Lysine 79 methylation (H3K79me) is enriched on gene bodies proportional to expression levels, and serves as a strong barrier for reprogramming of somatic cells induced pluripotent stem (iPSCs). DOT1L the sole histone methyltransferase that deposits all three orders - mono, di, tri – at H3K79. Here we leverage genetic chemical approaches parse specific functions higher H3K79me in maintaining cell identity. interacts with AF10 ( Mllt10 ) which recognizes unmodified H3K27...