A5046119400- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Ischemic Stroke Management
- Neurological Disease Mechanisms and Treatments
- Barrier Structure and Function Studies
- S100 Proteins and Annexins
- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Biomarkers in Disease Mechanisms
- Genetic Syndromes and Imprinting
- Inflammasome and immune disorders
- Reproductive Health and Technologies
- Epigenetics and DNA Methylation
Universitätsklinikum Würzburg
2021-2025
University of Würzburg
2022
Matrix metalloproteinases (MMPs) are implied in blood-brain barrier degradation and haemorrhagic transformation following ischaemic stroke, but their local relevance the hyperacute disease phase is unknown. We aimed to examine ultra-early MMP-9 MMP-2 release into collateral blood vessels, assess its prognostic value before therapeutic recanalisation by endovascular thrombectomy (EVT).
BACKGROUND: Ischemic stroke (IS) is a prevalent cause of death and disability worldwide. Cerebral ischemia induces profound changes at the blood-brain barrier, which lead to remarkable increase in paracellular permeability, worsening outcomes. Platelets are well known for safeguarding vascular integrity prevention bleeding complications. On other hand, platelet activation contributes infarct progression context IS. The manifold, context-dependent roles platelets, however, have not yet been...
The molecular basis of male infertility is poorly understood, the majority cases remaining unsolved. association aberrant sperm DNA methylation patterns and compromised semen parameters suggests that disturbances in germline epigenetic reprogramming contribute to this problem. So far there are only few data on heterogeneity within a given sample how select best for successful treatment. Limiting dilution bisulfite sequencing small pools from fertile donors did not reveal significant...
Ischemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization occluded vessels primary therapeutic aim, but even if it achieved, not all patients benefit. Although blockade platelet aggregation did prevent infarct progression, cerebral thrombosis as cause secondary growth has remained a matter debate. As thrombi are frequently observed after experimental thrombus-induced impairment brain microcirculation considered to...
In acute stroke, neuroinflammation can nowadays be analyzed by local cerebral aspiration of pial-ischemic blood during mechanical thrombectomy. Recently, Shaw et al. reported on differences in leukocyte subpopulations within the occluded cerebrovascular compartment. their study, a main proportion granulocytes was lost isolation. By immediate analysis, we found reproducible increase absolute without variations lymphocyte and monocyte numbers. Flow-cytometric phenotyping confirmed high shift...
Thrombolysis with recombinant tissue plasminogen activator (rt-PA) is a mainstay of acute ischemic stroke treatment but associated bleeding complications, especially after prolonged large vessel occlusion. Recently, inhibition the NLRP3 inflammasome led to preserved blood-brain barrier (BBB) integrity in experimental vivo. To further address potential as adjunct we used immortalized brain derived endothelial cells (bEnd5) an vitro model BBB. We treated bEnd5 rt-PA combination specific...
In large vessel occlusion stroke, recanalization to restore cerebral perfusion is essential but not necessarily sufficient for a favorable outcome. Paradoxically, in some patients, reperfusion carries the risk of increased tissue damage and hemorrhage. Experimental clinical data suggest that endothelial cells, representing interface detrimental platelet leukocyte responses, likely play crucial role phenomenon referred as ischemia/reperfusion (I/R)-injury, mechanisms are unknown. We aimed...
Ischemic stroke is associated with exacerbated tissue damage caused by the activation of immune cells and initiation other inflammatory processes. Dimethyl fumarate (DMF) known to modulate response, activate antioxidative pathways, improve blood–brain barrier (BBB) after stroke. However, specific impact DMF on cerebral ischemia remains unclear. In our study, male mice underwent transient middle artery occlusion (tMCAO) for 30 min received oral (15 mg/kg) or a vehicle immediately tMCAO,...
Abstract Ischemic stroke is among the leading causes of disability and death worldwide. In acute ischemic stroke, successful recanalization occluded vessels primary therapeutic aim, but even if it achieved, not all patients benefit. Although blockade platelet aggregation did prevent infarct progression, cerebral thrombosis as cause secondary growth has remained a matter debate. As thrombi are frequently observed after experimental thrombus-induced impairment brain microcirculation considered...