A5017102589- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Protein Structure and Dynamics
- Advanced NMR Techniques and Applications
- Force Microscopy Techniques and Applications
- Sphingolipid Metabolism and Signaling
- Nanopore and Nanochannel Transport Studies
- Metabolomics and Mass Spectrometry Studies
- Molecular Sensors and Ion Detection
- Photoreceptor and optogenetics research
- Electrostatics and Colloid Interactions
- Phagocytosis and Immune Regulation
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Lipid metabolism and biosynthesis
- Surfactants and Colloidal Systems
- RNA Interference and Gene Delivery
- Electrochemical Analysis and Applications
- Protease and Inhibitor Mechanisms
- Neuroscience and Neuropharmacology Research
- Fuel Cells and Related Materials
- Analytical Chemistry and Sensors
- 3D Printing in Biomedical Research
- Advanced Chemical Sensor Technologies
- Chemical and Physical Properties in Aqueous Solutions
Indiana University – Purdue University Indianapolis
2015-2024
Indiana University Indianapolis
2024
University of Indianapolis
2014-2023
Indiana University
2019
Indianapolis Zoo
2019
University of Arizona
2001-2015
Argonne National Laboratory
2011
National Institutes of Health
2003-2007
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2003-2007
Duke University
2005
The fundamental issue of interactions between lipid bilayers is addressed experimentally and theoretically. We report high-resolution x-ray scattering data for composed three different kinds phosphatidylcholine lipids. These yield the interbilayer water spacing fluctuation $\ensuremath{\sigma},$ as well traditional osmotic pressure $P,$ both functions lamellar repeat $D$ aqueous separation $a.$ show theoretically how to obtain functional form fluctuational free energy from...
The simplest, single-component biological membrane challenges accepted models of macromolecular interactions: lipid lamellar phases swell when immersed in monovalent salt solutions. Moreover, typical a Hofmeister series, Br salts multilayers more than Cl salts, offering an excellent opportunity to investigate long-standing questions ionic specificity. In accord with earlier measurements liposome mobilities electric fields, we find added electrostatic repulsion membranes due anion binding,...
Critical to biological processes such as membrane fusion and secretion, ion-lipid interactions at the membrane-water interface still raise many unanswered questions. Using reconstituted phosphatidylcholine membranes, we confirm here that multilamellar vesicles swell in salt solutions, a direct indication modifies between neighboring membranes. By varying sample histories, by comparing with data from ion carrier-containing bilayers, eliminate possibility swelling is an equilibration artifact....
Polyunsaturated phospholipids of the ω-3 and ω-6 classes play key roles in cellular functions, yet their mechanisms biological action are still a matter debate. Using deuterium (2H) NMR spectroscopy small-angle X-ray diffraction, we show how membrane properties modified by docosahexaenoic (DHA; 22:6) arachidonic (AA; 20:4) acyl chains families, respectively. Structural dynamical differences due to polyunsaturation evident both ordered disordered phases mixed-chain (16:0)(22:6)PC...
Cholesterol is an abundant lipid in eukaryotic membranes, implicated numerous structural and functional capacities. Here, we have investigated the mechanism by which cholesterol affects secretory granule biogenesis vivo using Dhcr7(-/-) Sc5d(-/-) mouse models of human diseases, Smith-Lemli-Opitz syndrome (SLOS) lathosterolosis. These homozygous-recessive multiple-malformation disorders are characterized absence one last two enzymes biosynthetic pathway, resulting accumulation precursors....
The dispersion of Nafion ionomer particles and Pt/C catalyst aggregates in liquid media was studied using both ultra-small-angle X-ray scattering (USAXS) cryogenic TEM. A systematic approach taken to study first the each component (i.e., aggregates), then combination components, last ink. Multiple-level curve fitting used extract particle size, size distribution, geometry from data. results suggest that are not uniform throughout systems but rather vary significantly. It found interaction...
Membranes with a high content of polyunsaturated phosphatidylethanolamines (PE) facilitate formation metarhodopsin-II (M(II)), the photointermediate bovine rhodopsin that activates G protein transducin. We determined whether M(II)-formation is quantitatively linked to elastic properties PEs. Curvature elasticity monolayers lipids 18 : 0-22 6(n - 3)PE, 5(n)- 6PE and model lipid 1(n 9)-18 1,(n- 9)PE were investigated in inverse hexagonal phase. All three form rather low spontaneous radii...
Membrane protein structure and function are influenced by the interaction with lipid bilayer environment. The dynamics in turn perturbed insertion. To study this mechanism, a number of experimental studies have used series model peptides (WALP) which consist sequences alternating alanine leucine amino acids terminated pair tryptophans at both ends. It has been shown that, due to hydrophobic mismatch, these can assume tilted conformations respect normal also perturb thickness. In an attempt...