A5102003502- Tuberculosis Research and Epidemiology
- Rabies epidemiology and control
- Mycobacterium research and diagnosis
- Helminth infection and control
- Parasites and Host Interactions
- Animal Virus Infections Studies
- Insect Resistance and Genetics
- Insurance, Mortality, Demography, Risk Management
- vaccines and immunoinformatics approaches
- Parasitic Infections and Diagnostics
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Dermatological diseases and infestations
- Animal Diversity and Health Studies
- Viral Infections and Vectors
- Bee Products Chemical Analysis
- Animal Nutrition and Physiology
- Vector-borne infectious diseases
- Virology and Viral Diseases
- Trace Elements in Health
- Global Maternal and Child Health
- Politics and Conflicts in Afghanistan, Pakistan, and Middle East
- Viral gastroenteritis research and epidemiology
- Global Health Care Issues
- Viral Infections and Outbreaks Research
Aga Khan University
2025
University of Veterinary and Animal Sciences
2019-2023
Huazhong Agricultural University
2017-2023
Bahauddin Zakariya University
2021
Jilin International Studies University
2021
Jilin University
2021
Pakistan Institute of Development Economics
2020
Pir Mehr Ali Shah Arid Agriculture University
2020
University of Agriculture Faisalabad
2019
Institute of Apiculture Research
2019
Recently, we reported the chloroplast genome-wide association of oligonucleotide repeats, indels and nucleotide substitutions in aroid genomes. We hypothesized that distribution repeat sequences a single representative genome can be used to identify mutational hotspots loci suitable for population genetic, phylogenetic phylogeographic studies. Using information on location repeats taro (Colocasia esculenta), designed 30 primer pairs amplify sequence polymorphic loci. The primers have been...
Abstract The co‐occurrence of mutational events including substitutions and insertions–deletions (InDels) with oligonucleotide repeats has previously been reported for a limited number prokaryotic, eukaryotic, organelle genomes. In this study, the correlations among these in chloroplast genomes species eudicot family Malvaceae were investigated. This study also genome sequences Hibiscus mutabilis , Malva parviflora Malvastrum coromandelianum . These three 16 other publicly available from 12...
Abstract Background Pneumonia is a major cause of childhood mortality in Pakistan. In 2019, the Government Pakistan revised national Integrated Management Childhood Illness (IMCI) chart booklet, following World Health Organization's recommendation for outpatient management with oral antibiotics children aged 2–59 months chest indrawing pneumonia. We aimed to assess outcomes pneumonia programme setting Methods This was prospective observational cohort study three primary health care...
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as major reservoir host, the MERS-CoV infection dynamics not fully understood. Through surveillance Pakistan, nasal (n = 776) and serum 1050) samples were collected from between November 2015 February 2018. Samples animal markets, free-roaming herds abattoirs. An in-house ELISA was developed to detect IgG against...
Abstract Mycobacterium tuberculosis is the causative agent of (TB), which still leading cause mortality from a single infectious disease worldwide. The development novel anti-TB drugs and vaccines severely hampered by complicated time-consuming genetic manipulation techniques for M. tuberculosis. Here, we harnessed an endogenous type III-A CRISPR/Cas10 system efficient gene editing RNA interference (RNAi). This simple easy method only needs to transform mini-CRISPR array plasmid, thus...
Abstract Mycobacterium tuberculosis ( M. ) can replicate in the macrophage by interfering with many host protein functions. While it is far from known these proteins for controlling infection. Herein, we infected macrophages including THP-1 and Raw264.7 cells identified differentially expressed genes (DEGs) interferon signaling pathway. Among them, 2′-5′ oligoadenylate synthetase-like (OASL) underwent greatest upregulation -infected macrophages. Knockdown of expression OASL attenuated...
Production and processing factors that affect native goat meat quality are non-existent in the literature. The current study explored age, sex, chilling effects on 48 carcasses cut half by halal standards shifting one immediately to a walk-in chiller at 0–4°C [rapid (RC)] other 26 ± 2°C [delayed (DC)]. Results showed Warner Bratzler Shear Force (WBSF) was significantly affected RC. Cooking loss color (L*) parameters were higher young male animals. Chroma values DC. In conclusion, DC enhances...
Sarcoptic mange was suspected in three of five European albino rabbits (Oryctolagus cuniculus) kept for experimental purposes. Gross examination revealed multifocal areas alopecia around the eyes, nostrils and lips. Skin snips were processed using PCR molecular identification mites. Histopathology skin showed erupted epidermis stratum corneum with an infiltration inflammatory cells. scraping presence adult mites as well eggs. Microscopic taxonomy identified Sarcoptes (S.) scabiei (Acari:...
This study was performed to determine the presence of West Nile virus (WNV) in mosquito specimens and human blood donors Pakistan.A total 4150 were collected using CO2-baited traps from five selected districts Punjab Province, Pakistan. The mosquitoes taxonomically identified standard morphological keys, resulting 166 pools. In addition, 1070 serum samples donors. RNA extracted screened for WNV a reverse transcriptase PCR (RT-PCR).None pools tested positive WNV, whereas three asymptomatic...
Flushing, Birth weight
Abstract Mycobacterium tuberculosis ( M.tb ) causes the current leading infectious disease. Examination of functional genomics and development drugs vaccines are hampered by complicated time-consuming genetic manipulation techniques for M.tb. Here, we reprogrammed endogenous type III-A CRISPR-Cas10 system simple efficient gene editing, RNA interference screening via delivery a plasmid harboring mini-CRISPR array, thereby avoiding introduction exogenous proteins minimizing proteotoxicity. We...
Abstract The current study was designed to check the anthelmintic activities of some local plants. Seeds Amomum (A.) subulatum and Vitex (V.) negundo in different solvents were subjected vitro (adult motility assay; AMA egg hatch EHA) vivo (faecal count reduction test; FECRT) activity testing protocols using Haemonchus (H.) contortus as an experimental model. results AMA, EHA, FECRT statistically analysed through linear regression Duncan multiple range test. In test, at 50 mg mL-1...
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Background: Among the blood-borne parasitic infections of dogs, trypanosomosis and babesiosis are highly prevalent in Indian subcontinent pathogenic. Methods: A six-month-old dog was presented with complaint lethargy, anorexia loss body weight over previous 15 days on October 11, 2021. full clinical targeted haematologic parasitological examination undertaken. Results: Wet smears were positive for motile trypanosomes while stained thin blood showed large number extracellular...
This study was conducted to compare the scrotal skin dynamics of camel and buffalo bulls regarding thermoregulation, season testosterone. Scrotal blood samples (n = 8 each) were collected during breeding (October March) non-breeding seasons (April August). Tissue slides prepared by paraffin embedding technique followed hematoxylin eosin (H&E) staining analysed ImageJ®. Relative humidity temperature recorded in different calculate thermal index (THI). Serum testosterone level estimated...