A5085212364- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- T-cell and B-cell Immunology
- Hemoglobinopathies and Related Disorders
- RNA Interference and Gene Delivery
- Erythrocyte Function and Pathophysiology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune cells in cancer
- CAR-T cell therapy research
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Erythropoietin and Anemia Treatment
- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Acute Lymphoblastic Leukemia research
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- HIV Research and Treatment
- Chronic Myeloid Leukemia Treatments
- Colorectal and Anal Carcinomas
- Psoriasis: Treatment and Pathogenesis
Sorbonne Université
2013-2023
Inserm
2012-2023
Centre d'Immunologie et des Maladies Infectieuses
2014-2023
Centre Hospitalier Universitaire de Poitiers
2017-2023
Centre National de la Recherche Scientifique
2008-2021
Sorbonne Paris Cité
2019
Université Sorbonne Nouvelle
2018
Pitié-Salpêtrière Hospital
1996-2015
Institut Universitaire de France
2013
Centre de Gestion Scientifique
2009
The diversity of the human immune repertoire and how it relates to a functional response has not yet been studied in detail humanized NOD.SCID.gammac(-/-) immunodeficient mice. Here, we used multiplex PCR on genomic DNA quantify combinatorial all possible V-J rearrangements at TCR-beta chain heavy Ig locus. We first show that generated thymus was well preserved periphery, suggesting T cells were vastly activated mice, agreement with phenotypic studies. then mice reached 100% reference...
By revisiting CD90, a GPI-anchored glycoprotein, we show that CD90 is expressed by subset of CD4(+) and CD8(+) human T cells. CD4(+)CD90(+) cells share similarities with Th17 because they express the Th17-specific transcription factor RORC2 produce IL-17A. are activated memory gut mucosal markers CCR6, CD161, α(4) β(7) integrins. Compared CD90-depleted CCR6(+) cells, higher levels IL-22 proinflammatory cytokines (IL-6, TNF-α GM-CSF), but lower IL-21 no IL-9. Analyses CD8(+)CD90(+) reveal...
Oral Squamous Cell Carcinomas (OSCC) are mostly related to tobacco consumption eventually associated alcohol (Smoker/Drinker patients: SD), but 25-30% of the patients have no identified risk factors (Non-Smoker/Non-Drinker NSND). We hypothesized that these distinguishable immune profiles could be useful for prognosis.Cells present in tumor microenvironment (TME) and blood from 87 OSCC HPV-negative were analyzed using a multiparameter flow cytometry assay, prospective case-control study....
Abstract Chronic exposure to environmental pollutants is often associated with systemic inflammation. As such, cigarette smoking contributes inflammation and lung diseases by inducing senescence of pulmonary cells such as pneumocytes, fibroblasts, endothelial cells. Yet, how worsens evolution chronic inflammatory disorders Th17 lymphocytes, rheumatoid arthritis, psoriasis, Crohn’s disease, multiple sclerosis, largely unknown. Results from human studies show an increase in CD4 + lymphocytes...
Abstract Background Lentiviral gene transfer into hematopoietic cells has been mostly optimized with vectors carrying a single reporter gene. For many clinical applications, lentiviral should contain more than one because transduced be enriched by selectable marker or killed for safety reasons after use. Thus, we compared various containing bicistronic cassette driven different ubiquitous promoters their ability to transduce human T‐lymphocytes, CD34 + ‐cells, and dendritic (DCs) derived...
Natural regulatory T cells (Tregs) may have a great therapeutic potential to induce tolerance in allogeneic and organ transplantations. In mice, we showed that alloantigen-specific Tregs (spe-Tregs) were more efficient than polyclonal (poly-Tregs) controlling graft-versus-host disease (GVHD). Here describe clinical-grade compliant method for generating human spe-Tregs. enriched from leukapheresis products with anti-CD25 immunomagnetic beads, primed twice by mature monocyte-derived dendritic...
The best methods for transducing hematopoietic progenitor cells usually involve either direct co-cultivation with virus-producing or human stromal supportive cells. However, these cannot be safely easily applied to clinical use. Therefore, we aimed at improving retrovirus-mediated gene transfer into progenitors derived from cord blood CD34+ using viral supernatant levels achieved least and under conditions that are suitable applications. In a first set of experiments, were infected...
Dendritic cells (DCs), the most potent antigen-presenting cells, can be generated from CD34+ hematopoietic stem and used for generating therapeutic immune responses. To develop immunotherapy protocols based on genetically modified DCs, we have investigated conditions high-level transduction of a large amount -derived DCs. Thus, an efficient clinically applicable protocol retroviral cord blood (CB) or mobilized peripheral (MPB) infection with gibbon ape leukemia virus (GALV)-pseudotyped...
The mechanism of polycythemia associated with the Budd–Chiari syndrome is unknown. Erythropoiesis in 10 patients was studied an attempt to distinguish prior unrecognized vera from secondary polycythemia. Serum erythropoietin assayed using a mouse fetal liver erythroblast assay. High concentrations serum were observed 6 7 acute primary syndrome. Levels normal four who investigated during chronic phase and increased one persisting In patient, concentration hepatic vein twice level measured...
Gene-directed enzyme prodrug therapy (GDEPT) consists in targeted delivery to tumor cells of a suicide gene responsible for situ conversion into cytotoxic metabolites. One the major limitations this strategy clinical application was poor activation capacity gene. We built highly efficient capable bioactivating cyclophosphamide (CPA) by fusing CYP2B6 triple mutant with NADPH cytochrome P450 reductase (CYP2B6TM-RED). Expression fusion via recombinant lentivirus (LV) vector converted resistant...
Abstract Background Human CD4 + CD25 FOXP3 natural regulatory T‐cells (nTreg) have a great therapeutic potential for the induction of tolerance in allo‐transplanted patients or control severe auto‐immune diseases. However, clinical‐grade production nTreg remains difficult to achieve because absence truly specific surface marker and their low frequency that implies need ex vivo expansion. Furthermore, safety issues should be taken into consideration due risk either uncontrolled nTreg‐induced...
Human papillomavirus (HPV) is involved in the development of anogenital tumors and also oropharyngeal head neck carcinomas, where HPV-16, expressing E6 E7 oncoproteins, most frequent serotype. Although vaccines encoding L1 L2 capsid HPV proteins are efficient for prevention infection, they inadequate treating established tumors. Hence, innovative vaccine therapies targeting E6/E7 important controlling HPV-induced cancers. We have engineered a nononcogenic mutated E7-specific plasmo-retroVLP...