A5000467636- Lipid Membrane Structure and Behavior
- Surfactants and Colloidal Systems
- Advanced Polymer Synthesis and Characterization
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Electrostatics and Colloid Interactions
- Endoplasmic Reticulum Stress and Disease
- Spectroscopy and Quantum Chemical Studies
- Protein Structure and Dynamics
- Spectroscopy and Chemometric Analyses
- Sphingolipid Metabolism and Signaling
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Plant Molecular Biology Research
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Photochemistry and Electron Transfer Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nanopore and Nanochannel Transport Studies
- Silicon Nanostructures and Photoluminescence
- Luminescence and Fluorescent Materials
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2016-2024
Czech Academy of Sciences
2005-2020
Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry
2008-2018
Czech Academy of Sciences, Institute of Physics
2016
Charles University
2003-2008
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a critically important regulatory lipid of the plasma membrane (PM); however, little known about how cells regulate PM PI(4,5)P2 levels. Here, we show that phosphatidylinositol 4-phosphate (PI4P)/phosphatidylserine (PS) transfer activity endoplasmic reticulum (ER)–resident ORP5 and ORP8 regulated by both PI4P PI(4,5)P2. Dynamic control ORP5/8 recruitment to occurs through interactions with N-terminal Pleckstrin homology domains adjacent...
The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For first time, performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) maintain exactly same experimental conditions for both which allows quantitative comparison these commonly used membranes....
Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge their physical state and structure. However, mapping viscosity diffusion in complex composition is currently a non-trivial technical challenge. We report fluorescence lifetime spectroscopy imaging (FLIM) meso-substituted BODIPY molecular rotor localised leaflet model various compositions. prepare large giant unilamellar vesicles (LUVs GUVs) containing phosphatidylcholine (PC) lipids...
Triphenylphosphonium cations strongly enhance the PL quantum yield of near infrared photoluminescent gold nanoclusters by metal-to-ligand charge transfer.
Phosphatidylinositol 4-kinase beta (PI4KB) is one of four human PI4K enzymes that generate phosphatidylinositol 4-phosphate (PI4P), a minor but essential regulatory lipid found in all eukaryotic cells. To convert their substrates, PI4Ks must be recruited to the correct membrane compartment. PI4KB critical for maintenance Golgi and trans network (TGN) PI4P pools, however, actual targeting mechanism TGN membranes unknown. Here, we present an NMR structure complex its interacting partner,...
Phosphatidylinositol (PI) is an essential structural component of eukaryotic membranes that also serves as the common precursor for polyphosphoinositide (PPIn) lipids. Despite recognized importance PPIn species signal transduction and membrane homeostasis, there still a limited understanding relationship between PI availability turnover subcellular pools. To address these shortcomings, we established molecular toolbox investigations distribution within intact cells by exploiting properties...
There is an urgent need for specific antiviral treatments directed against SARS-CoV-2 to prevent the most severe forms of COVID-19. By drug repurposing, affordable therapeutics could be supplied worldwide in present pandemic context. Targeting nucleoprotein N coronavirus a strategy impede viral replication and possibly other essential functions associated with N. The properties naproxen, non-steroidal anti-inflammatory (NSAID) that was previously demonstrated active Influenza A virus, were...
Abstract Spectra of individual silicon nanocrystals within porous Si grains are studied by the wide‐field imaging microspectroscopy and their ON – OFF blinking is detected confocal single‐photon‐counting microscopy. Observed spectral properties comprise all features reported before in differently prepared single (SiNCs). Former apparently contradictory results shown to be due different experimental conditions. When effect dark periods ( switching) removed common ultimate photoluminescence...
Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists four chromatin-interacting proteins that regulate gene expression. Each BET contains two N-terminal bromodomains, recognize acetylated histones, and C-terminal protein–protein interaction domain. Using genome-wide screen, we identify 1450 genes whose transcription regulated by Brd2. In addition, almost 290 change their alternative splicing pattern upon depletion. specifically localized at promoters target...
Although there were experimental indications that phospholipid bilayers hydrated with D2O express different biophysical properties compared hydration by ordinary H2O, a molecular concept for this behavior difference was only recently proposed dynamics simulations study [T. Róg et al., J. Phys. Chem. B, 2009, 113, 2378–2387]. Here we attempt to verify those theoretical predictions fluorescence measurements on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and...
The microscopic origin of the bright nanosecond blue-green photoluminescence (PL), frequently reported for synthesized organically terminated Si quantum dots (Si-QDs), has not been fully resolved, hampering potential applications this interesting material. Here a comprehensive study PL from alkyl-terminated Si-QDs 2-3 nm size, prepared by wet chemical synthesis is reported. Results obtained on ensemble and those single nano-object level are compared, they provide conclusive evidence that...
The polyelectrolyte behavior of kinetically frozen multimolecular polystyrene-block-poly(methacrylic acid), PS−PMA, micelles has been studied in detail by a combination light scattering, fluorescence correlation spectroscopy, and atomic force microscopy conductometry aqueous solutions at low ionic strength. Experimental studies reveal important intermicellar electrostatic interactions. experimental data are interpreted with help computer studies, which combine mechanistic lattice Monte Carlo...
Enteroviruses, members of the family picornaviruses, are most common viral infectious agents in humans causing a broad spectrum diseases ranging from mild respiratory illnesses to life-threatening infections. To efficiently replicate within host cell, enteroviruses hijack several factors, such as ACBD3. ACBD3 facilitates replication various enterovirus species, however, structural determinants recruitment sites poorly understood. Here, we present characterization interaction between and...
New thiophene-bodipy-labeled nucleotide was synthesized and used in polymerase synthesis of DNA probes sensitive to hybridization or protein–DNA interactions <italic>in vitro</italic>.
The hydration of the poly(oxyethylene) shell in polystyrene-block-poly(2-vinylpyridine)-block-poly(oxyethylene) micelles was investigated by monitoring solvent relaxation response a solvent-sensitive fluorophore (patman). It has been found that occurs on nanosecond time scale. Results for triblock copolymer have compared with those obtained polystyrene-block-poly(2-vinylpyridine) order to evaluate effect outer polyoxyethylene layer. Considerable pH-dependent changes units at...
Steady-state and time-resolved fluorescence measurements were used to study the relaxation of microenvironment hydrophobic probes 6-propionyl-2-(dimethylamino)naphthalene (prodan) 6-dodecanoyl-2-(dimethylamino)naphthalene (laurdan) in systems containing vesicles formed by amphiphilic diblock copolymer poly(epsilon-caprolactone)-block-poly(ethylene oxide) (PCL-PEO) water/tetrahydrofurane (THF) solvent mixtures. It was found that case prodan, both steady-state emission spectra composed two...
Summary Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort been dedicated to investigating functional relevance particular events, even those in key developmental and hormonal regulators. Combining approaches genetics, biochemistry advanced confocal microscopy, we describe impact on PIN7 gene model plant Arabidopsis thaliana . encodes a polarly localized transporter for phytohormone auxin produces...