A5038837637- Phytase and its Applications
- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Trace Elements in Health
- Wheat and Barley Genetics and Pathology
- Plant biochemistry and biosynthesis
- Animal Nutrition and Physiology
- Plant Pathogens and Fungal Diseases
- Biochemical and Structural Characterization
- Allelopathy and phytotoxic interactions
- Iron Metabolism and Disorders
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolites in Food Biotechnology
- Insect Resistance and Genetics
- Biochemical effects in animals
- Weed Control and Herbicide Applications
- Mycotoxins in Agriculture and Food
- Phytochemicals and Antioxidant Activities
- Genomics, phytochemicals, and oxidative stress
- Genetically Modified Organisms Research
- Plant and Biological Electrophysiology Studies
Aarhus University
2015-2025
BOKU University
2011
Aalborg University
2011
University of Copenhagen
2008
University of Catania
1995
The cisgenesis concept implies that plants are transformed only with their own genetic materials or from closely related species capable of sexual hybridization. Furthermore, foreign sequences such as selection genes and vector-backbone should be absent. We used a barley phytase gene (HvPAPhy_a) expressed during grain filling to evaluate the in barley. marker elimination method was obtain marker-free plant lines. Here, interest flanked by T-DNA borders allow unlinked integration two genes....
Barley (Hordeum vulgare) and wheat (Triticum aestivum) possess significant phytase activity in the mature grains. Maize (Zea mays) rice (Oryza sativa) little or virtually no preformed grain depend fully on de novo synthesis during germination. Here, it is demonstrated that wheat, barley, maize, all purple acid phosphatase (PAP) genes that, expressed Pichia pastoris, give functional phytases (PAPhys) with very similar enzyme kinetics. Preformed PAPhy was localized to protein crystalloid of...
Phytases increase utilization of phytate phosphorus in feed. Since wheat is rich endogenous phytase activity it was examined whether phytases could improve degradation compared to microbial phytases. Moreover, investigated enzymatic influenced by the matrix surrounding it. Phytate defined as decrease sum InsP₆ + InsP₅.Endogenous effectively degraded Ins₆ InsP₅ at pH 4 and 5, while this not true for a recombinant or extracted from bran. Only were able degrade entire range 3 which relevant...
Abstract Programmed cell death (PCD) in multicellular organisms is a vital process growth, development, and stress responses that contributes to the formation of tissues organs. Although numerous studies have defined molecular participants apoptotic PCD cascades, successful identification early master regulators target specific cells live or die limited. Using Zea mays somatic embryogenesis as model system, we report expressions two plant hemoglobin (Hb) genes (ZmHb1 ZmHb2) regulate...
Abstract Background The potyviruses sugarcane mosaic virus (SCMV) and maize dwarf (MDMV) are major pathogens of worldwide. Two loci, Scmv1 Scmv2 , have ealier been shown to confer complete resistance SCMV. Custom-made microarrays containing previously identified SCMV candidate genes gene analogs were utilised investigate validate expression patterns isogenic lines under pathogen infection in order obtain information about the molecular mechanisms involved maize-potyvirus interactions....
Anti-nutritional factors in plant seeds diminish the utilization of nutrients feed and food. Among these, protease inhibitors inhibit protein degradation by exogenous proteases during digestion. Through conventional selection-gene-free genome editing using ovules as explants, we used simplex multiplex CRISPR/Cas9 for studying impact chymotrypsin inhibitor CI-1A, CI-1B CI-2, Bowman-Birk trypsin inhibitor, Serpin-Z4, barley ɑ-amylase/subtilisin on soybean storage degradation. Mutants were...
Summary At present, little is known about the phytases of plant seeds in spite fact that this group enzymes primary determinant for utilization major phosphate storage compound seeds, phytic acid. We report cloning and characterization complementary DNAs (cDNAs) encoding one groups with phytase activity, multiple inositol phosphatases (MINPPs). Four wheat cDNAs ( TaPhyIIa1 , TaPhyIIa2 TaPhyIIb TaPhyIIc ) three barley HvPhyIIa1 HvPhyIIa2 HvPhyIIb were isolated. The open reading frames ranged...
The phytase purple acid phosphatase (HvPAPhy_a) expressed during barley seed development was evaluated as transgene for overexpression in barley. constitutively driven by the cauliflower mosaic virus 35S-promoter, and activity measured mature grains, green leaves dry vegetative plant parts left after harvest of grains. T
Antisense- or RNAi-mediated suppression of the biosynthesis nutritionally inferior storage proteins is a promising strategy for improving amino acid profile seeds. However, potential pleiotropic effects this on interconnected pathways and agronomic quality traits need to be addressed. In current study, transcriptomic analysis an antisense C-hordein line barley was performed, using grain-specific cDNA array. The characterized by marked changes in protein profiles, while seed weight within...
The phytase activity in food and feedstuffs is an important nutritional parameter. Members of the Triticeae tribe accumulate purple acid phosphatase phytases (PAPhy) during grain filling. This accumulation elevates mature activities (MGPA) up to levels between ~650 FTU/kg for barley 6000 rye. notably more than other cereals. For instance, rice, maize, oat have MGPAs below 100 FTU/kg. cloning characterization PAPhy gene complement from wheat, barley, rye, einkorn, Aegilops tauschii reported...
Around 70% of total seed phosphorus is represented by phytate which must be hydrolysed to bioavailable in non-ruminant diets. The limited endogenous phytase activity animals make it common practice add an exogenous source most poultry and pig feeds. mature grain (MGPA) cereal seeds provides a route for the themselves contribute digestion, but MGPA varies considerably between species varieties current use negligible contributions. Currently, all phytases used feed supplementation transgenic...
Fusarium head blight (FHB) causes substantial losses of yield and quality in grains, both the field post-harvest storage. To date, adequate natural genetic resistance is not available for control FHB. This study reports cloning overexpression a barley (Hordeum vulgare L.) antifungal gene, nepenthesin 1 (HvNEP-1), endosperm grains. Transgenic lines overexpressing HvNEP-1 substantially reduced FHB severity disease progression after inoculation with graminearum or culmorum. The transgenic also...
In the current study, we report for first time that grain components of barley, rice, wheat and maize can inhibit activity Aspergillus ficuum phytase. The phytase inhibition is dose dependent varies significantly between cereal species, cultivars barley Fusarium graminearum infected non-infected grains. highest endpoint level was 90%, observed with protein extracts (GPE) from F. wheat. Wheat GPE grains inhibits more than For four studied, IC50 value ranged 0.978 ± 0.271 to 3.616 0.087...
Background: Zinc accumulates in the embryo, aleurone, and subaleurone layers at different amounts cereal grains. Our hypothesis is that zinc could be stored bound, not only to low MW metabolites/proteins, but also high proteins as well. Methods: In order identify most abundant binding grain tissues, we microdissected barley grains into (1) seed coats; (2) aleurone/subaleurone; (3) embryo; (4) endosperm. Initial screening for putative from tissue types was performed by fractionating according...
is an entomopathogenic fungus that grows both in vivo and vitro. In it can colonize live insect hosts, tissue digestion occurs by secreted hydrolytic exoenzymes. It also dead provided this free from competing microorganisms. Depending on whether the host alive or expression (quality/quantity) of exoenzymes may vary. We have grown several isolates