A5016434182- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Immune cells in cancer
- Infectious Diseases and Mycology
- Immune Response and Inflammation
- Bacterial Genetics and Biotechnology
- Inflammatory mediators and NSAID effects
- Helicobacter pylori-related gastroenterology studies
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Quinazolinone synthesis and applications
- Immune responses and vaccinations
- Invertebrate Immune Response Mechanisms
- Genomics and Phylogenetic Studies
- Infectious Diseases and Tuberculosis
- Immunodeficiency and Autoimmune Disorders
- Asthma and respiratory diseases
- Microbial Natural Products and Biosynthesis
- Nanopore and Nanochannel Transport Studies
- Viral gastroenteritis research and epidemiology
- Antimicrobial Peptides and Activities
Niigata University
2014-2025
Fujita Health University
2024
Airlangga University
2024
University of Minnesota Medical Center
2024
Japan Anti Tuberculosis Association
2024
Niigata University Medical and Dental Hospital
2020-2023
Toneyama National Hospital
2022
National Hospital Organization
2022
Florida Atlantic University
2005-2011
Kyowa Kirin (Japan)
2010
The ST5 lineage of methicillin-resistant Staphylococcus aureus (MRSA) is one the most globally disseminated hospital-associated MRSA (HA-MRSA) lineages. We isolated a new local variant (designated ST764) over at least 5 years that causes invasive infections, including necrotizing fasciitis, and carried by medical students, as well household members. Analysis genome sequence isolate compared to reference strain revealed ST764 had acquired virulence traits similar those community-associated...
Methicillin-resistant Staphylococcus aureus (MRSA) is a common multidrug-resistant (MDR) pathogen. We herein discussed MRSA and its infections in Krasnoyarsk, Siberian Russia between 2007 2011. The incidence of 3,662 subjects was 22.0% 2.9% for healthcare- community-associated (HA- CA-MRSA), respectively. 15-day mortality rates hospital- community-acquired pneumonia (HAP CAP) were 6.5% 50%, CAP cases included pediatric deaths; the episodes available, ≥27.3% associated with bacteremia. Most...
Abstract Survival of the live attenuated Bacillus Calmette-Guérin (BCG) vaccine amidst harsh host environments is key for BCG effectiveness as it allows continuous immune response induction and protection against tuberculosis. Mycobacterial DNA binding protein 1 (MDP1), a nucleoid associated protein, essential in BCG. However, there limited knowledge on extent MDP1 gene regulation how this influences survival. Here, we demonstrate that conditional knockdown (cKD) grows slower than vector...
Human intelectin-1 (hITLN-1) is a 120-kDa lectin recognizing galactofuranosyl residues found in cell walls of various microorganisms but not mammalian tissues. Although mouse (mITLN-1) has been identified previously, its biochemical properties and functional characteristics have studied. Therefore, we compared structures saccharide-binding specificities hITLN-1 mITLN-1 using recombinant proteins produced by cells. Recombinant trimer, disulfide-linked through Cys-31 Cys-48, N-glycosylated at...
ABSTRACT Murine Mφ that phagocytose CMP develop into M1; this response depends on the size and chemical composition of particles. In contrast, recent studies concluded chitin particles induce M2 eosinophil migration, promoting acquired Th2 immune responses against chitin-containing microbes or allergens. This study examined whether these apparently inconsistent to could be induced by variation in We compared with CMP, LCB, Sephadex G-100 beads (>40 μm). Beads were given i.p. WT mice...
Abstract Mycobacterium tuberculosis ( Mtb ) strains of Beijing lineage have caused great concern because their rapid emergence drug resistance and worldwide spread. DNA mutation rates that reflect evolutional adaptation to host responses the appearance not been elucidated in human-infected strains. We tracked obtained an original isolate from 1999 outbreak Japan, as well five other isolates spread humans, two patient recurrence. Three were patients who developed TB within one year after...
Tuberculosis (TB) is one of the leading causes death due to infectious disease. The sole established vaccine against TB Mycobacterium bovis Bacillus Calmette–Guerin (BCG) vaccine. However, owing lack durable immunity with BCG and its risk infection, safer vaccines that can also be used as boosters are needed. Here, we examined whether membrane vesicles (MVs) from (BCG-MVs) isolated statically cultured in nutrient-restricted Sauton’s medium (s-MVs) planktonically nutrient-rich commonly...
Methicillin-resistant Staphylococcus aureus (MRSA) emerged in 1961, just after the introduction of methicillin as a countermeasure against penicillinase-producing "multidrug-resistant" S. aureus, threat at that time. Since then, MRSA has posed continuous to medical care major multidrug-resistant pathogen hospitals. In 1997–1999, severe invasive infection with occurred community, and this attracted attention community-associated (CA-MRSA). The evolutionary steps include species-to-species...
Bacteria can proliferate perpetually without ageing, but they also face conditions where must persist. Mycobacteria survive for a long period. This state appears during mycobacterial diseases such as tuberculosis and leprosy, which are chronic develop after long-term persistent infections. However, the fundamental mechanisms of living mycobacteria unknown. Every Mycobacterium species expresses Mycobacterial DNA-binding protein 1 (MDP1), histone-like nucleoid associated protein. smegmatis is...
Abstract Background Mycobacterium intracellulare is a major etiological agent of avium-intracellulare pulmonary disease (MAC-PD). However, the characteristics virulence M. and in vivo chemotherapeutic efficacy remain unclear. In this study, we examined nine strains with different clinical phenotypes genotypes C57BL/6 mice. Results We classified three types (high, intermediate, low) based on kinetics bacterial load, histological lung inflammation, neutrophilic infiltration. High showed more...
Methicillin-resistant Staphylococcus aureus (MRSA) not only causes disease in hospitals, but also the community. The characteristics of MRSA transmission environment remain uncertain. In this study, were isolated from public transport Tokyo and Niigata, Japan. Of 349 trains examined, eight (2.3%) positive for MRSA. belonged to sequence types (STs) 5, 8, 88, 89, included community infection-associated ST8 (with novel type IV staphylococcal cassette chromosome mec) ST5 New York/Japan hospital...
Abstract Background Mycobacterium intracellulare is a representative etiological agent of emerging pulmonary M. avium-intracellulare complex disease in the industrialized countries worldwide. The recent genome sequencing clinical strains isolated from has provided insight into genomic characteristics pathogenic mycobacteria, especially for avium ; however, remain to be elucidated. Results In this study, we performed comparative analysis 55 and related such as paraintracellulare (MP), indicus...
Methicillin-resistant Staphylococcus aureus (MRSA) includes hospital-acquired MRSA (HAMRSA) and community-acquired (CA-MRSA). Panton-Valentine leukocidin (PVL)-positive multilocus sequence type 30 (ST30) is one of worldwide CA-MRSA, which has also persisted in Japan since the 1980s. However, unexpectedly, it was not same ST30 clone throughout. Before 2000, HA-MRSA with spa43 φSa3sea (phage Sa3 carrying sea gene) only PVL-positive Japan; 1980s, accounted for 23.5% HA-MRSA, showed multidrug...
The effects of Lactobacillus johnsonii La1 (LC1) on Helicobacter pylori colonization in the stomach were investigated. H. and gastritis LC1-inoculated Mongolian gerbils significantly less intense than those control animals. LC1 culture supernatant (>10-kDa fraction) inhibited motility induced bacterial aggregation human gastric epithelial cells, suggesting potential clinical use product.
The global incidence of the human nontuberculous mycobacteria (NTM) disease is rapidly increasing. However, knowledge gene essentiality under optimal growth conditions and relevant to natural ecology NTM, such as hypoxia, lacking. In this study, we utilized transposon sequencing comprehensively identify genes essential for in Mycobacterium intracellulare. Of 5126 M. intracellulare ATCC13950, 506 were identified genes, which 280 158 shared with tuberculosis marinum, respectively. included...
Campylobacter jejuni has recently been noted as the most common cause of bacterial foodborne diseases in Japan. In present study, we determined ST types C. and coli isolated from chickens patients with enteritis or GBS Japan Thailand. chickens, enteritis, exhibited divergent included several novel addition to worldwide types. was also divergent. Novel may represent unidentified native clones each country. Pulsed-field gel electrophoresis confirmed above typing demonstrated long-term...
Delamanid (DLM), a nitro-dihydroimidazooxazole derivative currently approved for pulmonary multidrug-resistant tuberculosis (TB) therapy, is prodrug activated by mycobacterial 7,8-didemethyl-8-hydroxy 5-deazaflavin electron transfer coenzyme (F 420 )-dependent nitroreductase (Ddn). Despite inhibiting the biosynthesis of subclass mycolic acids, active DLM metabolite remained unknown. Comparative liquid chromatography-mass spectrometry (LC-MS) analysis metabolites revealed covalent binding...
Abstract The architecture of the genome influences functions DNA from bacteria to eukaryotes. Intrinsically disordered regions (IDR) eukaryotic histones have pivotal roles in various processes gene expression. IDR is rare bacteria, but interestingly, mycobacteria produce a unique histone-like protein, MDP1 that contains long C-terminal IDR. Here we analyzed role function. By employing Mycobacterium smegmatis inducibly expresses or its IDR-deficient mutant, observed induces IDR-dependent...