A5053713471- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- DNA and Nucleic Acid Chemistry
- Crystallography and molecular interactions
- Legume Nitrogen Fixing Symbiosis
- NF-κB Signaling Pathways
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Catalysis and Hydrodesulfurization Studies
- Osteomyelitis and Bone Disorders Research
- Bone Metabolism and Diseases
- Chromosomal and Genetic Variations
- Catalysis for Biomass Conversion
- Cell death mechanisms and regulation
- Acute Lymphoblastic Leukemia research
- Electrocatalysts for Energy Conversion
University of Toronto
2020-2023
Columbia University Irving Medical Center
2016
Pennsylvania State University
2009-2014
Seoul National University
1987-2005
Abstract This unit describes the ChIP‐exo methodology, which combines chromatin immunoprecipitation (ChIP) with lambda exonuclease digestion followed by high‐throughput sequencing. allows identification of a nearly complete set binding locations DNA‐binding proteins at near‐single‐nucleotide resolution almost no background. The process is initiated cross‐linking DNA and associated proteins. Chromatin then isolated from nuclei subjected to sonication. Subsequently, an antibody against desired...
Tumor suppressor p53 regulates transcription of stress-response genes. Many targets remain undiscovered because uncertainty as to where binds in the genome and fact that few genes reside near p53-bound recognition elements (REs). Using chromatin immunoprecipitation followed by exonuclease treatment (ChIP-exo), we associated with 2,183 unsplit REs. REs were positionally constrained other regulatory elements, which may reflect structurally organized interactions. Surprisingly, stress resulted...
The lifetime prevalences of DSM-III mental disorders using Korean version DIS-III are presented. They were studied in 5,100 adults (aged 18 to 65) household selected by two stage cluster sampling. Comparisons made between regions, sex and age groups. International comparison with Epidemiologic Catchment Area program was also made.
Abstract Mammalian genomes comprise largely intergenic noncoding DNA with numerous cis -regulatory elements. Whether and how the size of affects gene expression in a tissue-specific manner remain unknown. Here we show that genes extended regions are preferentially expressed neural tissues but repressed other mice humans. Extended contain twice as many active enhancers compared to tissues. Neural globally co-expressed neighboring controlled by distinct shared regions. Moreover, generic...
Functional activation of β-catenin/Tcf signaling plays an important role in the early events colorectal carcinogenesis. We examined effect ionomycin against colon cancer cells. Reporter gene assay showed that inhibited efficiently. In addition, inhibition by HEK293 cells transiently transfected with a constitutively mutant β-catenin gene, whose product is not phosphorylated GSK3β, indicates its inhibitory mechanism related to itself or downstream components. To investigate precise mechanism,...
Identification of specific protein-DNA interactions on the genome is important for understanding gene regulation. Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) widely used to identify genome-wide binding locations DNA-binding proteins. However, ChIP-seq method limited by its heterogeneity in length sonicated DNA fragments and non-specific background DNA, resulting low mapping resolution uncertainty sites. To overcome these limitations, combination ChIP...
Identification of specific protein-DNA interactions on the genome is important for understanding gene regulation. Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) widely used to identify genome-wide binding locations DNA-binding proteins. However, ChIP-seq method limited by its heterogeneity in length sonicated DNA fragments and non-specific background DNA, resulting low mapping resolution uncertainty sites. To overcome these limitations, combination ChIP...