A5050056813- Hearing, Cochlea, Tinnitus, Genetics
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Neural dynamics and brain function
- Advanced Electron Microscopy Techniques and Applications
- Plant Molecular Biology Research
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Pain Mechanisms and Treatments
- RNA Research and Splicing
- Advanced Fluorescence Microscopy Techniques
- Vestibular and auditory disorders
- Lipid Membrane Structure and Behavior
- Molecular Biology Techniques and Applications
- Neuroscience and Neural Engineering
- Hearing Loss and Rehabilitation
- RNA regulation and disease
- Erythrocyte Function and Pathophysiology
- Biochemical Analysis and Sensing Techniques
- Retinal Development and Disorders
- Plant and Biological Electrophysiology Studies
- Exercise and Physiological Responses
- Digital Holography and Microscopy
- Veterinary Oncology Research
Innsbruck Medical University
2009-2024
Collaborative Research Group
2024
Czech Academy of Sciences, Institute of Physiology
2024
Universitätsmedizin Göttingen
2015-2023
University of Göttingen
2015-2023
Max Planck Institute for Multidisciplinary Sciences
2022
Max Planck Institute of Experimental Medicine
2016-2021
University of Bern
2020
University of Reading
2011-2014
Universität Innsbruck
2014
Resource16 November 2017Open Access Source DataTransparent process Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy Katharina N Richter Department of Neuro- Sensory Physiology, University Göttingen Medical Center, Göttingen, Germany Cluster Excellence Nanoscale Microscopy Molecular Physiology the Brain, Search for more papers by this author Natalia H Revelo orcid.org/0000-0003-2492-1317 J Seitz International Max Planck Research School...
Interleukin-6 (IL-6) is a key mediator of inflammation. Inhibitors IL-6 or its signal transducing receptor gp130 constitute novel class anti-inflammatory drugs, which raise great hopes for improved treatments painful inflammatory diseases such as rheumatoid arthritis. and may enhance pain not only indirectly through their proinflammatory actions but also direct action on nociceptors (i.e., neurons activated by stimuli). We found indeed that the IL-6/gp130 ligand-receptor complex induced heat...
Ribbon synapses of cochlear inner hair cells (IHCs) undergo molecular assembly and extensive functional structural maturation before hearing onset. Here, we characterized the nanostructure IHC from late prenatal mouse embryo stages (embryonic days 14-18) into adulthood [postnatal day (P)48] using electron microscopy tomography as well optical nanoscopy apical turn organs Corti. We find that synaptic ribbon precursors arrive at presynaptic active zones (AZs) after afferent contacts have been...
Levetiracetam (LEV) is a prominent antiepileptic drug that binds to neuronal synaptic vesicle glycoprotein 2A protein and has reported effects on ion channels, but with poorly defined mechanism of action. We investigated inhibition voltage-dependent Ca<sup>2+</sup> (Ca<sub>V</sub>) channels as potential through which LEV exerts activity. used electrophysiological methods investigate the cholinergic transmission Ca<sub>V</sub> channel activity in superior cervical ganglion neurons (SCGNs). In...
Virus-mediated expression of channelrhodopsin enables optogenetic stimulation auditory pathways and restoration auditory-driven behavior in deaf adult gerbils.
Ribbon synapses of cochlear inner hair cells (IHCs) employ efficient vesicle replenishment to indefatigably encode sound. In neurons, neuroendocrine and immune cells, depends on proteins the mammalian uncoordinated 13 (Munc13) Ca2+-dependent activator for secretion (CAPS) families, which prime vesicles exocytosis. Here, we tested whether Munc13 CAPS also regulate exocytosis in mouse IHCs by combining immunohistochemistry with auditory systems physiology IHC patch-clamp recordings mice...
When hearing fails, electrical cochlear implants (eCIs) provide the brain with auditory information. One important bottleneck of CIs is poor spectral selectivity that results from wide current spread each electrode contacts. Optical (oCIs) promise to make better use tonotopic order spiral ganglion neurons (SGNs) inside cochlea by spatially confined stimulation. Here, we established multichannel oCIs based on light-emitting diode (LED) arrays and used them for optical stimulation...
Glycoprotein 130 (gp130) is the signal transducing receptor subunit for cytokines of interleukin-6 (IL-6) family, and it expressed in a multitude cell types immune nervous system. IL-6-like are not only key regulators innate immunity inflammation but also essential factors differentiation development somatosensory Mice with null mutation gp130 primary nociceptive afferents (<i>SNS-gp130</i><sup>−/−</sup>) largely protected from hypersensitivity to mechanical stimuli mouse models pathological...
Hearing impairment is the most common sensory disorder in humans. So far, rehabilitation of profoundly deaf subjects relies on direct stimulation auditory nerve through cochlear implants. However, some forms genetic hearing impairment, organ Corti structurally intact and therapeutic replacement mutated gene could potentially restore near natural hearing. In case defects otoferlin ( OTOF ), such therapy hindered by size coding sequence (~6 kb) exceeding cargo capacity (&lt;5 preferred...
At the presynaptic active zone (AZ), related cytomatrix proteins CAST and ELKS organize release machinery. While is known to regulate AZ size neurotransmitter release, role of integral system CAST/ELKS together poorly understood. Here, we show that have both redundant unique roles in coordinating synaptic development, function, maintenance retinal photoreceptor ribbon synapses. A double knockout (dKO) mouse showed high levels ectopic synapses reduced responses visual stimulation. Ectopic...
The cochlea of our auditory system is an intricate structure deeply embedded in the temporal bone. Compared with other sensory organs such as eye, has remained poorly accessible for investigation, example, by imaging. This limitation also concerns further development technology restoring hearing case cochlear dysfunction, which requires quantitative information on spatial dimensions and sensorineural status cochlea. Here, we employed X-ray phase-contrast tomography light-sheet fluorescence...
Tenderness and mechanical allodynia are key symptoms of malignant tumor, inflammation neuropathy. The proinflammatory cytokine interleukin-6 (IL-6) is causally involved in all three pathologies. IL-6 not only regulates innate immunity but also causes nociceptor sensitization hyperalgesia. In general most cell types including immune cells sensory neurons, binds soluble μ receptor subunits which heteromerizes with membrane bound signal transducer gp130. the present study, we used a conditional...
The afferent synapses between inner hair cells (IHC) and spiral ganglion neurons are specialized to faithfully encode sound with sub-millisecond precision over prolonged periods of time. Here, we studied the role Rab3 interacting molecule-binding proteins (RIM-BP) 1 2 – multidomain active zone known directly interact RIMs, Bassoon Ca V 1.3 in IHC presynaptic function hearing. Recordings auditory brainstem responses otoacoustic emissions revealed that genetic disruption RIM-BPs mice (...
Synaptic vesicle glycoprotein (SV)2A is a transmembrane protein found in secretory vesicles and critical for Ca(2+) -dependent exocytosis central neurons, although its mechanism of action remains uncertain. Previous studies have proposed, variously, role SV2 the maintenance formation readily releasable pool (RRP) or regulation responsiveness primed vesicles. Such previous typically used genetic approaches to ablate levels; here, we strategy involving small interference RNA (siRNA) injection...
The inner ear uses specialized synapses to indefatigably transmit sound information from hair cells spiral ganglion neurons at high rates with submillisecond precision. emerging view is that cell achieve their demanding function by employing an unconventional presynaptic molecular composition. Hair active zones hold the synaptic ribbon, electron-dense projection made primarily of RIBEYE, which tethers a halo vesicles and thought enable large readily releasable pool contribute its rapid...
Sensory synapses are characterized by electron-dense presynaptic specializations, so-called synaptic ribbons. In cochlear inner hair cells (IHCs), ribbons play an essential role as core active zone (AZ) organizers, where they tether vesicles, cluster calcium channels and facilitate the temporally-precise release of primed vesicles. While a multitude studies aimed to elucidate molecular composition function IHC ribbon synapses, developmental formation these signalling complexes remains...
Abstract Sensory synapses are characterized by electron-dense presynaptic specializations, so-called synaptic ribbons. In cochlear inner hair cells (IHCs), ribbons play an essential role as core active zone (AZ) organizers, where they tether vesicles, cluster calcium channels and facilitate the temporally-precise release of primed vesicles. While a multitude studies aimed to elucidate molecular composition function IHC ribbon synapses, developmental formation these signalling complexes...
In mammals, spatial orientation is synaptically-encoded by sensory hair cells of the vestibular labyrinth. Vestibular (VHCs) harbor synaptic ribbons at their presynaptic active zones (AZs), which play a critical role in molecular scaffolding and facilitate release vesicular replenishment. With advancing age, prevalence deficits increases; yet, underlying mechanisms are not well understood possible accompanying morphological changes VHC synapses have yet been systematically examined. We...