A5056091050- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Polysaccharides Composition and Applications
- Sunflower and Safflower Cultivation
- Plant Physiology and Cultivation Studies
- African Botany and Ecology Studies
- Soybean genetics and cultivation
- Agriculture and Rural Development Research
- Photosynthetic Processes and Mechanisms
- Plant Micronutrient Interactions and Effects
- Biocrusts and Microbial Ecology
- Fern and Epiphyte Biology
- Plant and animal studies
- Plant Parasitism and Resistance
- Glycosylation and Glycoproteins Research
- Plant pathogens and resistance mechanisms
- Food composition and properties
- Carbohydrate Chemistry and Synthesis
- Magnetic and Electromagnetic Effects
Normandie Université
2015-2025
Université de Rouen Normandie
2015-2025
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2013-2024
Laboratoire Physiologie Cellulaire & Végétale
2009-2022
Université Paris Cité
2008-2021
Délégation Paris 7
2008-2021
Laboratoire Interdisciplinaire des Énergies de Demain
2018
Sorbonne Paris Cité
2018
Institute for Research and Innovation in Biomedicine
2014
University of Cape Town
2007-2008
Abstract During plant sexual reproduction, pollen germination and tube growth require development under tight spatial temporal control for the proper delivery of sperm cells to ovules. Pollen tubes are fast growing tip-polarized able perceive multiple guiding signals emitted by female organ. Adhesion via cell wall molecules may be part battery signals. In order study these processes, we investigated characteristics in vitro-grown Arabidopsis (Arabidopsis thaliana) using a combination...
Abstract Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated role PECTIN METHYLESTERASE48 (PME48), an enzyme implicated remodeling pectins Arabidopsis (Arabidopsis thaliana) pollen. A combination functional genomics, gene expression, vivo and vitro germination, immunolabeling, biochemical analyses was used on wild-type Atpme48 mutant plants. showed that AtPME48 specifically expressed male gametophyte...
Changes in catalase (CAT) activity and CAT isoform pattern expression were investigated developing sunflower (Helianthus annuus L.) seeds during desiccation on the mother plant after artificial drying flowerheads. Seeds regularly desiccated their development reached mass maturity at c. 42 d flowering (DAF). Freshly harvested did not germinate any stage of because they dormant, but dormancy was broken 5-6 months dry storage. Immature desiccation-tolerant 24 DAF since able to fully flowerheads...
As there is limited information on the mechanisms of vegetative desiccation tolerance in pteridophytes, we undertook a comprehensive anatomical, ultrastructural, physiological and biochemical study fern Mohria caffrorum. Our data show that this species desiccation-tolerant during dry season, desiccation-sensitive rainy season. This system allows verification protection by comparison tolerant sensitive tissues same at developmental age. Tolerant fronds acquire drying are mostly similar to...
GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts to GDP-L-galactose, and is considered be a central enzyme connecting the major ascorbate biosynthesis pathway primary cell wall metabolism in higher plants. Our previous work demonstrated that GME crucial for both tomato. The aim of present study was investigate respective role two SlGME genes tomato by targeting each them through an RNAi-silencing approach. Taken individually SlGME1 SlGME2 allowed normal accumulation leaf fruits, thus...
Rhamnogalacturonan-II (RG-II) is one of the pectin motifs found in cell wall all land plants. It contains sugars such as 2-keto-3-deoxy-d-lyxo-heptulosaric acid (Dha) and 2-keto-3-deoxy-d-manno-octulosonic (Kdo), within RG-II mostly a dimer via borate diester cross-link. To date, little known regarding biosynthesis this motif. Here, after brief review our current knowledge on structure, function plants, study explores implications presence Golgi-localized sialyltransferase-like 2 (SIA2)...
UDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides and required for production seed mucilage. Following synthesis in cytosol, it transported into lumen Golgi apparatus, where converted to UDP-galacturonic (UDP-GalA), UDP-arabinose, UDP-xylose. To identify Golgi-localized UDP-GlcA transporter, we screened Arabidopsis thaliana mutants genes coding putative nucleotide sugar transporters altered mucilage, a structure rich GalA-containing polysaccharide...
The fine-tuning of the degree methylesterification cell wall pectin is a key to regulating elongation and ultimately shape plant body. Pectin spatiotemporally controlled by methylesterases (PMEs; 66 members in Arabidopsis [Arabidopsis thaliana]). comparably large number proteinaceous methylesterase inhibitors (PMEIs; 76 Arabidopsis) questions specificity PME-PMEI interaction functional role such abundance. To understand difference, or redundancy, between PMEIs, we used molecular dynamics...
In plants, 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) is a monosaccharide that only found in the cell wall pectin, rhamnogalacturonan-II (RG-II). Incubation of 4-day-old light-grown Arabidopsis seedlings or tobacco BY-2 cells with 8-azido 8-deoxy Kdo (Kdo-N3 ) followed by coupling to an alkyne-containing fluorescent probe resulted specific muro labelling RG-II through copper-catalysed azide-alkyne cycloaddition reaction. CMP-Kdo synthetase inhibition and competition assays showing D-Ara,...
The pathogenicity of various Streptomyces scabies isolates involved in potato scab disease was correlated with the production thaxtomin A. Since calcium is known as an essential second messenger associated pathogen-induced plant responses and cell death, it investigated whether A could induce a Ca2+ influx related to death other putative using Arabidopsis thaliana suspension cells, which convenient model study plant-microbe interactions. cells were treated micromolar concentrations Cell...
Oxalic acid is thought to be a key factor of the early pathogenicity stage in wide range necrotrophic fungi. Studies were conducted determine whether oxalate could induce programmed cell death (PCD) Arabidopsis thaliana suspension cells and detail transduction signalling pathway induced by oxalate. treated with millimolar concentrations Cell was quantified ion flux variations analysed from electrophysiological measurements. Involvement anion channel ethylene signal leading PCD determined...
SUMMARY Plant cell wall remodeling plays a key role in the control of elongation and differentiation. In particular, fine‐tuning degree methylesterification pectins was previously reported to developmental processes as diverse pollen germination, tube elongation, emergence primordia or dark‐grown hypocotyls. However, how pectin degradation can modulate plant development has remained elusive. Here we report characterization polygalacturonase (PG), AtPGLR, gene for which is highly expressed at...
Date palm is an important economical crop in many countries. However, recent years, the Kebili region with largest groves of Tunisia faced deficit rain fall that can impaired maturation and quality fruits especially non‐irrigated plantation. Water does not stop fruit development but has a dramatic effect on growth, pulp content cell wall composition remodeling during maturation. The data indicate was more hydrophilic suggesting these subtle changes property may participate maintaining water...
Abstract Despite its low abundance, rhamnogalacturonan-II (RG-II) is an essential structural component of the cell wall and present in a highly conserved molecular configuration across all plants. RG-II branched pectin domain that contains 13 different sugars linked by over 20 bond types, uniquely among pectins it can be covalently dimerized via borate diesters. hypothesized to crosslink matrix, controlling architecture porosity, but has resisted detailed analyses due compositional...
Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice adapted fluorescent probes for live cell imaging. What detect in what type sample and how long is major issue with and, this, “hat-trick” “Probe–Sample–Instrument” (PSI) has be considered. We propose here deal key points usually discussed CIPs properties organic probes, modality labeling, best equipment obtain appropriate spectral, spatial, temporal resolution....
Protein tracking in living plant cells has become routine with the emergence of reporter genes encoding fluorescent tags. Unfortunately, this imaging strategy is not applicable to glycans because they are directly encoded by genome. Indeed, complex result from sequential additions and/or removals monosaccharides glycosyltransferases and glycosidases cell's biosynthetic machinery. Currently, cell wall polymers mainly relies on use antibodies or dyes that exhibit variable specificities....
Oxalic acid is thought to be a key factor of the early pathogenic stage in wide range necrotrophic fungi. We have recently published that oxalic induces Programmed Cell Death (PCD) Arabidopsis thaliana cells. This cell death results from an anionic efflux which prerequisite for synthesis ethylene and PCD. Complementary experiments been carried out by using seedlings A. thaliana. The effects millimolar concentrations were analysed on seedlings. A treatment with 3 mM solution does not alter...
Plant sexual reproduction involves the growth of tip-polarized pollen tubes through female tissues in order to deliver sperm nuclei egg cells. Despite importance this crucial step, little is known about molecular mechanisms involved spatial and temporal control tube growth. In study process characterize structural composition extracellular matrix male gametophyte, immunocytochemical biochemical analyses Arabidopsis wall have been carried out. Results showed a well defined localization cell...
Background and Aims In flowering plants, fertilization relies on the delivery of sperm cells carried by pollen tube to ovule. During tip growth tube, proper assembly cell wall polymers is required maintain mechanical properties wall. Xyloglucan (XyG) a polymer known for maintaining integrity thus allowing expansion. most angiosperms, XyG somatic fucosylated, except in Asterid clade (including Solanaceae), where fucosyl residues are replaced arabinose, presumably due an adaptive and/or...