A5080733569- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Metabolomics and Mass Spectrometry Studies
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Radical Photochemical Reactions
- Photopolymerization techniques and applications
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- Luminescence and Fluorescent Materials
- Chemical Synthesis and Reactions
- Photochromic and Fluorescence Chemistry
- CO2 Reduction Techniques and Catalysts
- Microfluidic and Capillary Electrophoresis Applications
- Synthesis and Catalytic Reactions
- Carbon and Quantum Dots Applications
- Sulfur-Based Synthesis Techniques
- Quantum Dots Synthesis And Properties
- Catalytic C–H Functionalization Methods
- Electrochemical Analysis and Applications
New Jersey Institute of Technology
2020-2024
China Pharmaceutical University
2018-2019
State Key Laboratory of Natural Medicine
2018
Recent studies have shown that ultrafast enzymatic digestion of proteins can be achieved in microdroplet within 250 μs. Further investigation peptides resulting from (MD) would necessary to evaluate it as an alternative the conventional bulk for bottom-up and biotherapeutic protein characterization. Herein we examined compared tryptic both MD solution. In case β-lactoglobulin B, preservation long was observed due short time. addition, is applicable digest high- low-abundance mixture....
Microdroplet reactions have aroused much interest due to significant reaction acceleration (e.g., ultrafast protein digestion in microdroplets could occur less than 1 ms). This study integrated a microdroplet technique with automated sample flow injection and online mass spectrometry (MS) analysis, develop rapid robust method for structural characterization of monoclonal antibodies (mAbs) that is essential assess the antibody drug's safety quality. Automated sequential aspiration mixing an...
Photobase generators (PBGs) are compounds that utilize light-sensitive chemical-protecting groups to offer spatiotemporal control of releasing organic bases upon targeted light irradiation. PBGs can be implemented as an external initiate anionic polymerizations such thiol-ene Michael addition reactions. However, there limitations for common PBGs, including a short absorption wavelength and weak base release lead poor efficiency in photopolymerization. Therefore, is great need...
The capture of reactive intermediates is important for the elucidation reaction mechanisms. We report first observation electrochemically generated, short-lived radical cations carbazole (t1/2 ≈ 97 μs) and two N-substituted derivatives by mass spectrometry. In addition, online investigation reactivity generated supports that dimerization mechanism involves one cation with neutral molecule rather than previously proposed coupling cations.
Proteomic absolute quantitation strategies mainly rely on the use of synthetic stable isotope-labeled peptides or proteins as internal standards, which are highly costly and time-consuming to synthesize. To circumvent this limitation, we recently developed a coulometric mass spectrometry (CMS) approach for without based electrochemical oxidation oxidizable surrogate peptides, followed by measurement peptide yield. Previously, CMS was only applied single-protein quantitation. In study, first,...
Abstract In this study, we developed a novel simple fluorescence resonance‐energy transfer (FRET) system between two‐color CdTe quantum dots (QDs) assisted by cetyltrimethylammonium bromide (CTAB). Mercaptopropionic (MPA)‐capped QDs serving as both donors and acceptors were successfully synthesized changing the refluxing time in aqueous solution. CTAB micelles formed water minimized distance significantly electrostatic interactions, improving FRET efficiency. Several factors that affected...
Isotope-labeled internal standards are routinely used for mass spectrometry (MS)-based absolute quantitation. However, syntheses of isotope-labeled peptides time-consuming and costly. To tackle this issue, we recently developed a coulometric spectrometric (CMS) approach quantitation without the use standards, based on electrochemical oxidation cysteine or tyrosine-containing followed by measurement yield. further expand utility method, herein present CMS method tryptophan oxidation. Several...
Currently, glycopeptide quantitation is mainly based on relative due to absolute requiring isotope-labeled or standard glycopeptides which may not be commercially available are very costly and time consuming synthesize. To address this grand challenge, coulometric mass spectrometry (CMS), the combination of electrochemistry (EC) (MS), was utilized quantify electrochemically active without need using materials. In study, we studied tyrosine-containing glycopeptides, NYIVGQPSS(β-GlcNAc)TGNL-OH...
Alkyl isothiocyanates (R-NCS) have pharmacological applications and provide a synthetic handle to various functional groups including thioureas. There are however few methods access alkyl through the creation of C-N bond. We developed simple approach for conjugate isothiocyanation enones by trimethylsilyl isothiocyanate (TMSNCS), which proceeds 1,4-addition weak nucleophile activated in absence external promoters. This method avoids direct use highly toxic acids bases, produces...