From the cell envelope preparation of Sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. The high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are only glycolipids this lipopolysaccharide-lacking gram-negative bacterium. low-density showed many minor bands proteins sodium dodecyl sulfate-polyacrylamide gel electrophoresis, NADH oxidase activity was...

The occurrence of deoxynivalenol (DON) was evaluated in 113 wheat samples from the northern and central/southwestern regions Paraná State, Brazil during 2008 2009 growing seasons, this rate used to estimate DON dietary exposure. determination carried out by an indirect competitive enzyme-linked immunosorbent assay. detected 66.4% at levels ranging 206.3 4732.3 μg/kg (mean 1894.9 μg/kg). estimated daily intake (EDI) through bread pasta inhabitants Londrina City Brazil. average these 0.79 body...

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on an anti-aflatoxin B1 monoclonal antibody was standardised and validated for aflatoxin screening in poultry feed samples its performance compared to high-performance liquid chromatography (HPLC). The ic-ELISA showed good linearity (r2 = 0.994) detection limits of 1.25 ng g−1 broiler 1.41 laying hen feed. Mean recovery rates by were 102% (laying feed) 98% (broiler feed). Aflatoxins detected 88.2% the 34 HPLC at means...

Taking into account the preliminary antagonistic/biodegradation property showed by Pichia membranifaciens and Sporobolomyces roseus, which decreased initial patulin concentration of 588.4 to 290.0 µg/mL, ability P. ohmeri 158 in biocontrol against Penicillium expansum decrease vitro was performed. The culture supernatant effective 66.17% micelial growth, indicating antibiosis related with killer phenomenon. 223 µg presence cells over 83% original concentration, when incubated at 25ºC/2 days...

Fusarium verticillioides is a primary corn pathogen and fumonisin producer which associated with toxic effects in humans animals. The traditional methods for detection of fungal contamination based on morphological characteristics are time-consuming show low sensitivity specificity. Therefore, the objective this study was to develop PCR-ELISA FUM21 gene F. detection. DNA verticillioides, sp., Aspergillus sp. Penicillium isolates analyzed by conventional PCR determine specific isolates,...

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) HRP-anti IgG (10³-fold dilution). The limit 3.73 ng OTA/g correlation coefficients (r) between this immunoassay high performance liquid chromatography 0.98 green coffee, 0.86 instant. OTA levels detected by...

Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim present study was identify that contaminate inside coffee beans collected in stage maturation drying, from 16 producing areas located northern region State Paraná, South Brazil. total 108 isolates spp. identified at level, by sequencing internal transcribed spacer (ITS1-5.8S-ITS2) ribosomal DNA (rDNA)....

The effect of fludioxonil + metalaxyl-M on the mycelial morphology, sporulation and fumonisin B 1 production by Fusarium verticillioides 103 F was evaluated. Scanning electron microscopy analysis showed that fungicide caused inhibition hyphal growth defects hyphae morphology such as cell wall disruption, withered hyphae, excessive septation. In addition, extracellular material around rarely observed in presence metalaxyl-M. While promoting reduction growth, increased F. compared to control,...

Natural fungal and fumonisin contamination were evaluated in 109 freshly harvested corn samples from Paraná State correlated to damaged kernels (%). In addition, healthy of 24 selected order compare the mycoflora profile levels. The correlation among protein/lipid content levels was also analyzed 15 most frequently cultivated hybrids. Total colony count ranged 1.9x10(4) 3.5x10(6) CFU/g, Fusarium sp. 1.0x10³ 2.2x10(6) 0.13 20.38 µg/g. colony/Fusarium showed positive (p < 0.05). there a...

The performance of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) for ochratoxin A (OTA) detection was evaluated in comparative study with high-performance liquid chromatography (HPLC) analysis using 68 freshly harvested coffee samples from the North Paraná State, Brazil. anti-OTA mAb showed high specificity and low cross-reactivity OTA analogues (OTB OTα), but cross-reacted OTC. This ic-ELISA limit 3.75 ngg−1 sample, when compared...

The aim of this study was to evaluate the fate fumonisins B1 (FB1) and B2 (FB2) during industrial dry-milling in two lots from 2014 (n=120) 2015 non-transgenic corn their fractions (germ, pericarp, endosperm, cornmeal grits), collected one major Brazilian milling industries. Fumonisins were concentrated germ pericarp at a rate 322% 188% (lot 1) 311% 263% 2), respectively. In grits fumonisin levels decreased 60 95%. Fumonisin below maximum limit tolerated by European Commission. Therefore,...

The antifungal effect of the yeast species Kluyveromyces marxianus, Meyerozyma caribbica, and Wickerhamomyces anomalus was evaluated against two Fusarium graminearum strains (FRS 26 FSP 27) in vitro on corn seeds.The yeasts F. using scanning electron microscopy extracellular chitinase glucanase production to further elucidate biocontrol mode action. In addition, germination percentage vigor test were investigated after applying seeds. All inhibited fungal growth (57.4%-100.0%) seeds...

Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} O objetivo desse artigo foi levantar os principais fatores de risco intoxicação por agrotóxicos a que...

In this study the bright greenish-yellow fluorescence test, widely used by corn milling industry, was compared to thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 samples naturally contaminated Aspergillus section Flavi. According processing industry criteria, all were adequate human animal consumption but TLC analysis detected aflatoxins above maximum tolerated limit (20 µg/kg) 7 8 samples, respectively. Aflatoxins 16 (40%) TLC, with levels...

Brazil is one of the world's largest corn producers and a leader in exportation. Due to intense globalization, may be commercialized worldwide issue concerning safety corn-based products has become topic widespread international interest. Dietary exposure evaluation relevant criterion for mycotoxin risk assessment. Thus, human fumonisins were assessed grain its derivatives (endosperm, cornmeal, grits; n = 320) sampled from large-scale processing plants Brazil. The total probable daily intake...