A5077359614- Connexins and lens biology
- Advanced Glycation End Products research
- Heme Oxygenase-1 and Carbon Monoxide
- Chronic Kidney Disease and Diabetes
- Advanced biosensing and bioanalysis techniques
- Smoking Behavior and Cessation
- Diabetes Treatment and Management
- Diabetes and associated disorders
- Renal Diseases and Glomerulopathies
- Dermatologic Treatments and Research
- Heat shock proteins research
- Inflammasome and immune disorders
- Nicotinic Acetylcholine Receptors Study
- Neuroscience of respiration and sleep
- Adenosine and Purinergic Signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ureteral procedures and complications
- Telomeres, Telomerase, and Senescence
- RNA Interference and Gene Delivery
University of Lincoln
2019-2025
Without a viable cure, chronic kidney disease is global health concern. Inflammatory damage in and around the renal tubules dictates severity contributed to by multiple cell types. Activated response danger associated molecular patterns (DAMPs) including ATP, NOD-like receptor protein-3 (NLRP3) inflammasome integral this inflammation. In vivo, we have previously observed that increased expression of Connexin 43 (Cx43) linked inflammation (CKD) whilst vitro studies human proximal tubule cells...
Abstract Background Accompanied by activation of the NOD-like receptor protein 3 (NLRP3) inflammasome, aberrant connexin 43 (Cx43) hemichannel-mediated ATP release is situated upstream inflammasome assembly and inflammation contributes to multiple secondary complications diabetes associated cardiometabolic comorbidities. Evidence suggests there may be a link between Cx43 hemichannel activity in diabetic kidney. The consequences blocking tubular priming/activation NLRP3 model kidney disease...
Abstract Aims Following an acute myocardial infarction (AMI), individuals with type 2 diabetes (T2DM) have a 2‐to‐3 fold increased risk of mortality compared to those without diabetes, and globally cardiorenal complications account for 50% diabetes‐related deaths. The use sodium/glucose cotransporter‐2 inhibitors (SGLT2i) in people T2DM‐AMI is associated decreased inflammatory burden improved outcomes. mechanisms behind this protection are unclear form the basis study. Methods This single...
Chronic Kidney Disease (CKD) is associated with sustained inflammation and progressive fibrosis, changes that have been linked to altered connexin hemichannel-mediated release of adenosine triphosphate (ATP). fibrosis develops in response increased deposition extracellular matrix (ECM), up-regulation collagen I an early marker renal disease. With ECM remodeling known promote a loss epithelial stability, the current study we used clonal human kidney (HK2) model proximal tubular cells...
Abstract The ability of cells to communicate and synchronise their activity is essential for the maintenance tissue structure, integrity function. A family membrane‐bound proteins called connexins are largely responsible mediating local transfer information between cells. Assembled in cell membrane as a hexameric connexon, they either function conduit paracrine signalling, forming transmembrane hemi‐channel, or, if aligned with connexons on neighbouring cells, form continuous aqueous pore or...
Fibrosis of renal tubules is the final common pathway in diabetic nephropathy and develops face tubular injury fibroblast activation. Aberrant connexin 43 (Cx43) hemichannel activity has been linked to this damage under euglycaemic conditions, however, its role glycaemic unknown. This study investigated effect a Cx43 blocker (Tonabersat) on cell-cell interactions within between epithelial cells fibroblasts an vitro model nephropathy.Human kidney (HK2) proximal tubule medullary (TK173) were...
Connexins are membrane bound proteins that facilitate direct and local paracrine mediated cell-to-cell communication through their ability to oligomerise into hexameric hemichannels. When neighbouring channels align, they form gap-junctions provide a route for information transfer between cells. In contrast intact gap junctions, which typically open under physiological conditions, undocked hemichannels have low probability mainly in response injury. Hemichannels permit the release of small...