A5031275873- Microbial Natural Products and Biosynthesis
- Biochemical and Structural Characterization
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Hepatitis C virus research
- Advanced Biosensing Techniques and Applications
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- Lipid metabolism and disorders
- Peptidase Inhibition and Analysis
- Marine Sponges and Natural Products
- Photosynthetic Processes and Mechanisms
- Influenza Virus Research Studies
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
- Antibiotics Pharmacokinetics and Efficacy
- Phytochemical compounds biological activities
- Vibrio bacteria research studies
- Algal biology and biofuel production
- Peroxisome Proliferator-Activated Receptors
- Chemical Synthesis and Analysis
- interferon and immune responses
- Air Quality and Health Impacts
- Chemical synthesis and alkaloids
- Lipoproteins and Cardiovascular Health
- RNA and protein synthesis mechanisms
Pacific Northwest National Laboratory
2023-2024
University of Utah
2018-2022
Cyclic peptides are excellent drug candidates, placing macrocyclization reactions at the apex of development. PatG and related dual-action proteases from cyanobactin biosynthesis responsible for cleaving off C-terminal recognition sequence macrocyclizing substrate to provide cyclic peptides. This reaction has found use in enzymatic synthesis diverse macrocycles. However, these enzymes function best on substrates that terminate with non-proteinogenic thiazole/thiazoline residue, complicating...
Abstract Cyanobactins comprise a widespread group of peptide metabolites produced by cyanobacteria that are often diversified post‐translational prenylation. Several enzymes have been identified in cyanobactin biosynthetic pathways carry out chemically diverse prenylation reactions, representing resource for the discovery alkylating agents. Here, genome mining was used to identify orphan prenyltransferases, leading isolation tolypamide from freshwater cyanobacterium Tolypothrix sp. The...
Lipoproteins in cerebrospinal fluid (CSF) of the central nervous system (CNS) resemble plasma high-density lipoproteins (HDLs), which are a compositionally and structurally diverse spectrum nanoparticles with pleiotropic functionality. Whether CSF (CSF-Lps) exhibit similar heterogeneity is poorly understood because they present at 100-fold lower concentrations than HDL. To investigate diversity CSF-Lps, we developed sensitive fluorescent technology to characterize lipoprotein subspecies...
The methylation of peptide backbone amides is a hallmark bioactive natural products, and it also greatly modifies the pharmacology synthetic peptides. Usually, N-methylated peptides are cyclic. However, there very limited knowledge about how post-translational enzymes can be applied to synthesis designed or libraries. Here, driven by established ability some RiPP process diverse substrates, we sought define catalysts for in vivo vitro macrocyclization backbone-methylated We developed...
The emergence of novel viruses from zoonotic reservoirs are on the rise which can lead to outbreaks in humans that severely impact societal functions and global economy. Indeed, recent SARS-CoV 2 pandemic exposed vulnerabilities breakdowns our response time approaches rapidly temper these major threats. Thus, there is a dire need for identification druggable targets or pathways limit viral replication, transmission, and/or disease severity. Current systems biology utilize sophisticated mass...
The levels of circulating triglycerides (TG) carried in TG-rich lipoproteins (TGRL) and their remnants are causally associated with cardiovascular disease (CVD). Liberation TG from to peripheral tissues is modulated by TGRL-associated proteins including apolipoproteln A5 (ApoA5), which has been reported profoundly impact metabolism. Present at ~150 ng/ml plasma, it thought work via stimulation (or de-repression) lipoprotein lipase (LPL), hydrolyzes deliver fatty acids cells. To determine...
ABSTRACT Cyclic peptides are excellent drug candidates, placing macrocyclization reactions at the apex of development. PatG and related dual-action proteases from cyanobactin biosynthesis responsible for cleaving off C-terminal recognition sequence macrocyclizing substrate to provide cyclic peptides. This reaction has found use in enzymatic synthesis diverse macrocycles. However, these enzymes function best on substrates that terminate with non-proteinogenic thiazole/thiazoline residue,...
Abstract Cyanobactins comprise a widespread group of peptide metabolites produced by cyanobacteria that are often diversified post‐translational prenylation. Several enzymes have been identified in cyanobactin biosynthetic pathways carry out chemically diverse prenylation reactions, representing resource for the discovery alkylating agents. Here, genome mining was used to identify orphan prenyltransferases, leading isolation tolypamide from freshwater cyanobacterium Tolypothrix sp. The...
Acinetobacter baumannii has emerged as a troublesome nosocomial pathogen worldwide. We report here the draft genome sequence of polymyxin B-resistant type 195 (ST195) A. strain GU71, isolated from tertiary care hospital in city Guwahati, Assam, India.