The phosphorylation of photosystem II (PSII) and its antenna (LHCII) proteins has been studied, involvement in state transitions PSII repair is known. Yet, little known about the I (PSI) (LHCI) proteins. Here, we applied proteomics analysis to generate a map sites PSI-LHCI Chlorella ohadii cells that were grown under low or extreme high-light intensities (LL HL). Furthermore, analyzed content oxidized tryptophans protein degradation products these cells, estimate light-induced damage...

The search for new, non-standard targets is currently a high priority in the design of new antibacterial compounds. Bacterial toxin-antitoxin systems (TAs) are genetic modules that encode toxin protein causes growth arrest by interfering with essential cellular processes, and cognate antitoxin, which neutralizes activity. TAs have no human analogues, highly abundant bacterial genomes, therefore represent attractive alternative antimicrobial drugs. This study demonstrates how artificial...

Proton transfer (PT) is at the heart of fundamental natural biochemical reactions, e.g. in bioenergetics, where proteins are main proton mediators. PT between two specific points requires a change motive force via alteration acid-base properties. Nature solved this problem primarily by modulating protein structure during process. Here, we introduce light-triggered donor-bridge-acceptor approach for inducing and visualizing directional biosystems, specifically peptides. To do so, synthesize...

A library of eight new fluoroquinolone-nuclease conjugates containing a guanidinoethyl or aminoethyl auxiliary pendant on the 1,4,7-triazacyclononane (TACN) moiety was designed and synthesized to investigate their potential as catalytic antibiotics. The Cu(ii) complexes designer structures showed significant in vitro hydrolytic oxidative DNA cleavage activity good antibacterial against both Gram-negative Gram-positive bacteria. observed all Cu(ii)-TACN-ciprofloxacin strongly inhibited...

Vitamin B12 (or cobalamin) is an enzymatic cofactor essential both for mammals and bacteria. However, cobalamin can be synthesized only by few microorganisms so most bacteria need to take it up from the environment through TonB-dependent transport system. The first stage of import E. coli cells occurs outer-membrane receptor called BtuB. binds with high affinity extracellular side BtuB protein. forms a β-barrel inner luminal domain loops. To mechanically allow passage, needs partially unfold...

Short modified oligonucleotides that bind in a sequence-specific way to messenger RNA essential for bacterial growth could be useful fight infections. One such promising oligonucleotide is peptide nucleic acid (PNA), synthetic DNA analog with peptide-like backbone. However, the limitation precluding use of oligonucleotides, including PNA, bacteria do not import them from environment. We have shown vitamin B12, which most need take up growth, delivers PNAs Escherichia coli cells when...

Vitamin B12 also called cobalamin (Cbl) is an important enzymatic cofactor taken up by mammalian and many bacterial cells. Peptide nucleic acid (PNA) a synthetic DNA analogue that has the ability to bind in complementary manner natural acids. Provided PNA efficiently delivered cells, it could act as steric blocker of functional or RNA regulate gene expression at level transcription translation. Recently, Cbl been examined transporter various molecules Also, PNA, if covalently linked with...

New aminoglycosides containing 6′-carboxylic acid or 6′-amide were designed, synthesized and their ability to readthrough nonsense mutations was examined.

Abstract The emergence of multidrug‐resistant pathogens that are resistant to the majority currently available antibiotics is a significant clinical problem. development new antibacterial agents and novel approaches therefore extremely important. We set out explore potential catalytic as paradigm in research. Herein, we describe our pilot study on design, synthesis, biological testing series derivatives natural aminoglycoside antibiotic neomycin B for their action antibiotics. showed...

New derivatives of aminoglycosides with a side chain 1,2-aminoalcohol at the 5" position ring III were designed, synthesized, and biologically evaluated. The novel lead structure (compound 6), exhibiting substantially enhanced selectivity toward eukaryotic versus prokaryotic ribosome, high readthrough activity, considerably lower toxicity than previous compounds, was discovered. Balanced activity 6 demonstrated in three different nonsense DNA-constructs underlying genetic diseases, cystic...

Econazole, sulconazole and tioconazole usage as antifungal agents is limited due to poor pharmacokinetic properties. Pristine hydroxylated structures of the C240 fullerene single walled carbon nanotube (SWCNT) were proposed transporters these imidazoles potentially enhancing their pharmacokinetics.To assess possibility creation endohedral complexes azoles nanostructures, adsorption interaction energies calculated with hybrid exchange-correlation density functional B97-1 6-31(d,p) basis set....

A library of eight new fluoroquinolone–nuclease conjugates containing a guanidinoethyl or aminoethyl auxiliary pendant on the cyclen moiety was designed and synthesized to investigate their potential for overcoming general issue "metallodrug vulnerability" under physiological conditions. The Cu(II) Co(III) complexes designer compounds were synthesized, operate dynamic, intramolecular cap with DNase activity explored. lead Co(III)–cyclen–ciprofloxacin conjugate showed excellent in vitro...

Here we present new derivatives of nucleoside reverse transcriptase inhibitors with a C20 fullerene. The computational chemistry methods used in this study evaluate affinity designed compounds towards the HIV-1 (RT) binding site and select most active ones. best have superior or similar to RT comparison test compounds, including drugs anti-HIV therapy.

The phosphorylation of photosystem II (PSII) and its antenna (LHCII) proteins has been extensively studied involvement in state transitions PSII repair is well known. Yet, very little known about the extent functions I (PSI) (LHCI) proteins. Here, two proteomics methods were applied to generate a detailed map sites PSI-LHCI Chlorella ohadii cells that grown under low- or extreme high-light intensities (LL HL). Furthermore, we analyzed content oxidized tryptophans these cell types estimate...

A protonation state of serotonergic ligands plays a crucial role in their pharmacological activity. In this research, the basicity 8-phenylsulfanyl quipazine derivatives as new potential agents was studied. The most favorable sites were determined gas and aqueous phases. water, solvation effect promoting N3 atom overcomes positive charge delocalization phenomenon favoring N1 protonation. stable conformations neutral protonated molecules water found. It demonstrated that diprotonation...

Fullerene molecules are created entirely of carbon and form orbicular or ellipsoidal cage shape similar to a hollow tube. The derivatives fullerenes classified into the following categories according their functionalization: endohedral with active residing inside cage, exohedral wide variety both inorganic organic groups existing outside connected fullerenes’ exterior heterofullerenes when one more atoms that fullerene replaced by non-carbon atom, i.e. heteroatom. C 240 belongs family giant...

Fullerene molecules are created entirely of carbon and have a highly unusual attributes. The one the fullerene derivatives heterofullerenes when or more atoms that form cage replaced by non-carbon atom, i.e., heteroatom. aim study was to determine effect aromatic compound (benzene) on boron, silicon sulfur C 60 hetero stability. chosen heteroatoms were occupied only ring type. following complexes been studied: 6 H @C 54 B , 55 5 Si S . energies stabilization ( ΔE stab ), deformation def )...

Fullerene molecules are created entirely of carbon and form orbicular or ellipsoidal cage shape similar to a hollow tube. The derivatives fullerenes classified into the following categories according their functionalization: endohedral with active residing inside cage, exohedral wide variety both inorganic organic groups existing outside connected fullerenes’ exterior heterofullerenes when one more atoms that fullerene replaced by non-carbon atom, i.e. heteroatom. C 240 belongs family giant...