A5041392763- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- CAR-T cell therapy research
- Diet and metabolism studies
- Lymphoma Diagnosis and Treatment
- Biosimilars and Bioanalytical Methods
- Pharmacogenetics and Drug Metabolism
- Liver Disease Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Amyotrophic Lateral Sclerosis Research
- Alcoholism and Thiamine Deficiency
- Genetically Modified Organisms Research
- Viral Infectious Diseases and Gene Expression in Insects
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Phytochemicals and Antioxidant Activities
- Biochemical Acid Research Studies
- Skin Protection and Aging
- Liver Diseases and Immunity
- Liver Disease and Transplantation
- Genomics and Rare Diseases
- Chronic Kidney Disease and Diabetes
- Cholinesterase and Neurodegenerative Diseases
- Sulfur Compounds in Biology
- Epigenetics and DNA Methylation
Kite (United States)
2023-2025
Gilead Sciences (United States)
2023
Metabolon (United States)
2013-2022
Gilead Sciences (France)
2022
Natera (United States)
2021
Triangle
2018
Durham University
2011-2015
Universitätskinderklinik
1984
A molecular signature, derived from integrated analysis of clinical data, the metabolome, and proteome in prospective human studies, improved prediction death patients with sepsis, potentially identifying a subset who merit intensive treatment.
Metabolic profiling of cancer cells has recently been established as a promising tool for the development therapies and identification biomarkers. Here we characterized metabolomic profile human breast tumors uncovered intrinsic metabolite signatures in these using an untargeted discovery approach validation key metabolites. The oncometabolite 2-hydroxyglutarate (2HG) accumulated at high levels subset cell lines. We discovered association between increased 2HG MYC pathway activation cancer,...
A novel translational pharmacology investigation was conducted by combining an in vitro efficacy target with mucosal tissue pharmacokinetic (PK) data and mathematical modeling to determine the number of doses required for effective human immunodeficiency virus (HIV) preexposure prophylaxis (PrEP).
Axicabtagene ciloleucel (axi-cel) is an autologous anti-CD19 chimeric antigen receptor (CAR) T-cell therapy approved for relapsed/refractory (R/R) follicular lymphoma (FL). Approval was supported by the phase 2, multicenter, single-arm ZUMA-5 study of axi-cel patients with R/R indolent non-Hodgkin (iNHL; N = 104), including FL and marginal zone (MZL). In primary analysis (median follow-up, 17.5 months), overall response rate (ORR) 92% (complete rate, 74%). Here, we report long-term outcomes...
ZUMA-12 is a multicenter phase 2 study that evaluated axicabtagene ciloleucel (axi-cel), an autologous anti-CD19 chimeric antigen receptor (CAR) T-cell therapy, as part of first-line treatment in patients with high-risk large B-cell lymphoma (LBCL). In the primary efficacy analysis (n=37; 15.9 months median follow-up), axi-cel demonstrated high rate complete responses (CR; 78%) and safety profile consistent prior experience. Here, we assessed updated outcomes from 40 treated after ≥3 years...
Precision medicine, taking account of human individuality in genes, environment, and lifestyle for early disease diagnosis individualized therapy, has shown great promise to transform medical care. Nontargeted metabolomics, with the ability detect broad classes biochemicals, can provide a comprehensive functional phenotype integrating clinical phenotypes genetic nongenetic factors. To test application metabolomics individual diagnosis, we conducted analysis on plasma samples collected from...
The application of whole-exome sequencing for the diagnosis genetic disease has paved way systems-based approaches in clinical laboratory. Here, we describe a metabolomics method screening metabolic diseases through analysis multi-pronged mass spectrometry platform. By simultaneously measuring hundreds metabolites single sample, offers comprehensive approach to identify perturbations across multiple biochemical pathways.We conducted single- and multi-day precision study on human plasma 4,...
Bladder cancer (BCa) is a common malignancy worldwide and has high probability of recurrence after initial diagnosis treatment. As result, recurrent surveillance, primarily involving repeated cystoscopies, critical component post patient management. Since cystoscopy invasive, expensive possible deterrent to compliance with regular follow-up screening, new non-invasive technologies aid in the detection and/or primary bladder are strongly needed. In this study, mass spectrometry based...
Our objective was to identify metabolic pathways affected by ALS using non-targeted metabolomics in plasma, comparing samples from healthy volunteers those patients. This discovery could become the basis for identification of therapeutic targets and diagnostic biomarkers ALS. Two distinct cross-sectional studies were conducted. Plasma collected 62 (Study 1) 99 2) participants meeting El Escorial criteria possible, probable, or definite ALS; 69 48 controls collected. Global profiling used...
Our objective was to identify plasma biomarkers of ALS that can aid in distinguishing patients with from those disease mimics. In this multi-center study, samples were collected 172 recently diagnosed ALS, 50 healthy controls, and 73 neurological Samples analyzed using metabolomics. Using all identified biochemicals detected > 50% the metabolomics analysis, classified as or mimic 65% sensitivity 81% specificity by LASSO analysis (AUC 0.76). A subset panel 32 candidate these diagnosis groups...
A greater understanding of cholestatic disease is necessary to advance diagnostic tools and therapeutic options for conditions such as primary biliary cirrhosis (PBC) sclerosing cholangitis (PSC). The purpose this study was determine compare the serum metabolomes patients with PBC (n = 18) or PSC 21) healthy controls 10) identify metabolites that may differentiate these two diseases.Using a mass spectrometry-based, non-targeted biochemical profiling approach, we identified 420 metabolites,...
Abstract Genetically modified (GM) crops currently constitute a significant and growing part of agriculture. An important aspect GM crop adoption is to demonstrate safety equivalence with respect conventional crops. Untargeted metabolomics has the ability profile diverse classes metabolites thus could be an adjunct for substantial assessment. To account environmental effects introgression traits into genetic backgrounds, we propose that assessment metabolic composition should understood...
In metabolomics data, like other -omics normalization is an important part of the data processing. The goal to reduce variation from non-biological sources (such as instrument batch effects), while maintaining biological variation. Many techniques make adjustments each sample. One common method adjust sample by its Total Ion Current (TIC), i.e. for feature in sample, divide intensity value total Because many assumptions these methods are dubious sets, we compare two that separately...
We wished to determine the efficacy of using urine as an analyte screen for a broad range metabolic products associated with multiple different types inborn errors metabolism (IEMs), automated mass spectrometry-based assay. Urine was compared plasma samples from similar cohort analyzed same Specimens were two commonly utilized normalization methods based on creatinine and osmolality, respectively.Biochemical profiles each sample (from both affected unaffected subjects) obtained platform...
Because increasing evidence point to the convergence of environmental and genetic risk factors drive redox dysregulation in schizophrenia, we aim clarify whether metabolic anomalies associated with early psychosis reflect an adaptation oxidative stress. Metabolomic profiling was performed characterize response stress fibroblasts from control individuals (n = 20) patients 30), all, 282 metabolites were identified. In addition expected redox/antioxidant response, induced a decrease lysolipid...
Background: The oral glucose tolerance test (OGTT) is the only method to diagnose patients having impaired (IGT), but its use has diminished considerably in recent years. Metabolomic profiling studies have identified a number of metabolites whose fasting levels are associated with dysglycemia and type 2 diabetes. These may serve as basis an alternative for IGT. Method: Using stable isotope dilution technique, quantitative assays were developed 23 candidate biomarker metabolites. measured...
Introduction Colorectal cancer (CRC) is a highly prevalent disease, wherein, ~30%–40% of patients with CRC relapse postresection. In some CRC, adjuvant chemotherapy can help delay recurrence or be curative. However, current biomarkers show limited clinical utility in determining if/when should administered, to provide benefit. Circulating tumour DNA (ctDNA) measure molecular residual disease (MRD) and high specificity sensitivity. This study protocol investigates the ctDNA for optimal use...
Abstract Limonene is a lipophilic monoterpene found in high levels citrus peel. demonstrates anticancer properties preclinical models with effects on multiple cellular targets at varying potency. While of interest as cancer chemopreventive, the biologic activity limonene humans poorly understood. We conducted metabolite profiling 39 paired (pre/postintervention) plasma samples from early-stage breast patients receiving treatment (2 g QD) before surgical resection their tumor. Metabolite was...
Introduction Analysis of blood for the evaluation clinically relevant biomarkers requires precise collection and sample handling by phlebotomists laboratory staff. An important consideration clinical application metabolomics are different anticoagulants utilized collection. Most studies that have characterized differences in metabolite levels various tubes focused on single analytes. We define analyte a global platform following sampling using five different, but commonly used, (i.e., plasma...