A5081121290- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Immune Response and Inflammation
- Ultrasound and Hyperthermia Applications
- DNA and Nucleic Acid Chemistry
- Immune cells in cancer
- Nanoparticle-Based Drug Delivery
- SARS-CoV-2 and COVID-19 Research
- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Cavitation Phenomena
- Microbial Inactivation Methods
- Cystic Fibrosis Research Advances
- Mosquito-borne diseases and control
- Cancer Immunotherapy and Biomarkers
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Neonatal Respiratory Health Research
- Viral Infectious Diseases and Gene Expression in Insects
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- RNA and protein synthesis mechanisms
Ghent University
2016-2025
Koninklijke Nederlandse Maatschappij voor Diergeneeskunde
2025
Cancer Research Institute Ghent
2016-2024
Instituut voor Landbouw en Visserijonderzoek
2022
Ghent University Hospital
2012-2020
Geneeskundige en Gezondheidsdienst
2019
University of Veterinary Medicine
2015
AZ Sint-Lucas
2009
Rode Kruis-Vlaanderen
2006
Universidade de Santiago de Compostela
2006
Nonviral gene complexes can enter mammalian cells through different endocytic pathways. For efficient optimization of the carrier it is important to profile its cellular uptake, because this largely determines intracellular processing and subsequent transfection efficiency. Most current information on uptake these gene-delivery vehicles based data following use chemical inhibitors Here, we have performed a detailed characterization four commonly used endocytosis [chlorpromazine, genistein,...
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 200 leading journals. To access Abstract, please click on HTML or PDF.
Intracellular uptake and degradation of polyelectrolyte capsules is reported in this work. Two types intracellular degradable are described: enzyme-sensitive hydrolysis- sensitive (see figure). The results may be a significant step towards the biomedical application capsules.
Drug delivery with microbubbles and ultrasound is gaining more attention in the drug field due to its noninvasiveness, local applicability, proven safety ultrasonic imaging techniques. In this article, we tried improve cytotoxicity of doxorubicin (DOX)-containing liposomes by preparing DOX-liposome-containing for therapeutic ultrasound. way, DOX release uptake can be restricted ultrasound-treated areas. Compared DOX-liposomes, DOX-loaded killed at least two times melanoma cells after...
Understanding cellular uptake and intracellular processing of nonviral gene delivery systems is a key aspect in developing more efficient vectors. In this study, the impact clathrin- caveolae/lipid-raft-dependent endocytosis on cell entry overall transfection efficiency polyethylenimine (PEI) polyplexes was evaluated. Most remarkably, internalization pathway mediating successful depended both type polyplex applied. Colocalization studies with transferrin cholera toxin B revealed that at...
Ultrasound imaging is widely used in both diagnosis of diseases and pregnancy follow-up. As ultrasound a harmless, cheap portable technique, guided drug delivery gaining more attention the field. To be effective, most cases, ultrasonic makes use microbubbles. This highlight describes why it attractive to load microbubbles with drugs discusses recent attempts made design loaded
Cystic fibrosis (CF) is characterized by the presence of a viscoelastic mucus layer in upper airways and bronchi. The underlying problem mutation gene encoding cystic transmembrane conductance regulator protein. Clinical studies transfer for CF are ongoing. For delivery to patients be effective, covering target cells must overcome. We therefore examined extent which sputum presents physical barrier transport nanospheres size comparable that lipoplexes other transfection systems currently...
purpose. Intravitreal injection of therapeutic DNA, complexed to nonviral carriers such as cationic liposomes, may be promising in the treatment many severe retinal eye diseases. However, after intravitreal injection, DNA/cationic liposome complexes—called lipoplexes (LPXs)—which are typically hundreds nanometers size, must first diffuse through vitreous before they can reach retina. The aim this study was elucidate whether is a barrier for LPXs and find strategies overcome barrier. methods....
Ultrasound in combination with microbubbles has recently been considered by gene delivery scientists to be an interesting approach enhance transfer into cells. Its low toxicity and simplicity apply vivo without major complications make this technology (sonoporation) especially attractive. Sonoporation of DNA evaluated the injection free plasmid (pDNA) together (as used diagnostic imaging) bloodstream. However, gene-transfer efficiency these experiments remained rather low. Both enzymatic...
Accurate sizing of nanoparticles in biological media is important for drug delivery and biomedical imaging applications since size directly influences the nanoparticle processing nanotoxicity vivo. Using fluorescence single particle tracking we have succeeded first time following aggregation real undiluted whole blood. We demonstrate that, by using a suitable surface functionalization, blood circulation prevented to large extent.
Abstract Localized therapeutic modalities that subvert the tumor microenvironment from immune‐suppressive to pro‐immunogenic can elicit systemic antitumor immune responses induce regression of directly treated as well nontreated distal tumors. A key toward generating robust T cell is activation dendritic cells (DCs) in microenvironment. Treatment with agonists triggering various pattern recognition receptors very efficient activate DCs, yet suffers induction serious immune‐related adverse...
Given their high potential to evoke cytolytic T cell responses, tumor antigen-encoding messenger RNA (mRNA) vaccines are now being intensively explored as therapeutic cancer vaccines. mRNA clearly benefit from wrapping the into nano-sized carriers such lipoplexes that protect degradation and increase its uptake by dendritic cells in vivo. Nevertheless, early innate host factors regulate induction of lipoplex have remained unresolved. Here, we demonstrate induce a potent type I interferon...
Abstract Introduction Aberrant turnover of the actin cytoskeleton is intimately associated with cancer cell migration and invasion. Frequently however, evidence circumstantial, a reliable assessment therapeutic significance gene product offset by lack inhibitors that target biologic properties protein, as most conventional drugs do, instead corresponding gene. Proteomic studies have demonstrated overexpression CapG, constituent cytoskeleton, in breast cancer. Indirect suggests CapG involved...
Synthetic immune-stimulatory drugs such as agonists of the Toll-like receptors (TLR) 7/8 are potent activators antigen-presenting cells (APCs), however, they also induce severe side effects due to leakage from site injection into systemic circulation. Here, we report on design and synthesis an amphiphilic polymer-prodrug conjugate imidazoquinoline TLR7/8 agonist that in aqueous medium forms vesicular structures 200 nm. The contains endosomal enzyme-responsive linker enabling degradation...