A5078872209- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Chronic Myeloid Leukemia Treatments
- Gene expression and cancer classification
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Speech and Audio Processing
- Multiple Myeloma Research and Treatments
- RNA modifications and cancer
- Virus-based gene therapy research
- Music and Audio Processing
- Microbial Community Ecology and Physiology
- Ferroptosis and cancer prognosis
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Eosinophilic Disorders and Syndromes
- Monoclonal and Polyclonal Antibodies Research
Moffitt Cancer Center
2018-2025
Dalian Medical University
2024-2025
Chongqing Medical University
2024-2025
Second Affiliated Hospital of Chongqing Medical University
2024-2025
Shanxi University
2025
North University of China
2025
Heilongjiang Bayi Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2024
University of South China
2024
Shanghai Institute of Technology
2022-2023
Tumor-associated macrophages are major contributors to malignant progression and resistance immunotherapy, but the mechanisms governing their differentiation from immature myeloid precursors remain incompletely understood. In this study, we demonstrate that exosomes secreted by human mouse tumor-educated mesenchymal stem cells (MSCs) drive accelerated breast cancer inducing of monocytic myeloid-derived suppressor into highly immunosuppressive M2-polarized at tumor beds. Mechanistically,...
Abstract Most ovarian cancers are infiltrated by prognostically relevant activated T cells 1–3 , yet exhibit low response rates to immune checkpoint inhibitors 4 . Memory B cell and plasma infiltrates have previously been associated with better outcomes in cancer 5,6 but the nature functional relevance of these responses controversial. Here, using 3 independent cohorts that total comprise 534 patients high-grade serous cancer, we show robust, protective humoral dominated production...
The immune checkpoint receptor PD-1 on T follicular helper (Tfh) cells promotes Tfh:B cell interactions and appropriate positioning within tissues. Here, we examined the impact of regulation expression by genomic organizer SATB1 Tfh differentiation. Vaccination CD4CreSatb1f/f mice enriched for antigen-specific cells, TGF-β-mediated repression enhanced differentiation human cells. Mechanistically, high Icos in Satb1-/- CD4+ promoted preventing regulatory skewing resulted increased...
Despite repeated associations between T cell infiltration and outcome, human ovarian cancer remains poorly responsive to immunotherapy. We report that the hallmarks of tumor recognition in cancer-infiltrating cells are primarily restricted tissue-resident memory (TRM) cells. Single-cell RNA/TCR/ATAC sequencing 83,454 CD3
Melanoma brain metastases (MBM) and leptomeningeal melanoma (LMM) are two different manifestations of CNS metastasis. Here, we used single-cell RNA sequencing (scRNA-seq) to define the immune landscape MBM, LMM, skin metastases.scRNA-seq was undertaken on 43 patient specimens, including 8 metastases, 14 19 serial LMM specimens. Detailed cell type curation performed, landscapes were mapped, key results validated by IHC flow cytometry. Association analyses identify subsets correlated with...
Recent evidence demonstrates that the androgen receptor (AR) continues to influence prostate cancer growth despite medical therapies reduce circulating ligands castrate levels and/or block ligand binding. Whereas mutation, amplification, overexpression of AR, or cross-talk between AR and other factor pathways may explain failure ablation in some cases, there is little supporting a causal role cancer. In this study, we functionally directly address whereby contributes spontaneous progression...
Abstract Castration-resistant prostate cancer (CRPC) is a lethal stage of disease in which androgen receptor (AR) signaling persistent despite deprivation therapy (ADT). Most studies have focused on investigating cell-autonomous alterations CRPC, while the contributions tumor microenvironment are less well understood. Here we sought to determine role tumor-associated macrophages based upon their progression and therapeutic resistance. In syngeneic model that reflected mutational landscape...
Dimeric IgA (dIgA) can move through cells via the IgA/IgM polymeric immunoglobulin receptor (PIGR), which is expressed mainly on mucosal epithelia. Here, we studied ability of dIgA to target commonly mutated cytoplasmic oncodrivers. Mutation-specific dIgA, but not IgG, neutralized KRAS
Abstract Despite functional heterogeneity, high frequency of intratumoral neutrophils predicts poor clinical outcomes. The tumor microenvironment reprograms into immunosuppressive subsets that hinder anti-cancer immunity, thereby contributing to growth and resistance immunotherapies. However, the mechanisms underlying neutrophil reprogramming remain elusive. Here, we report ability brain tumor-infiltrating was restricted a highly glycolytic long-lived subset expressing CD71, which acquired...
Recent studies suggest that B cells could play an important role in the tumor microenvironment. However, of humoral responses endometrial cancer remains insufficiently investigated. Using a cohort 107 patients with different histological subtypes carcinoma, we evaluated coordinated and cellular adaptive immune cancer. Concomitant accumulation T, B, plasma at beds predicted better survival. only B-cell markers corresponded prolonged survival specifically high-grade endometrioid type serous...
Background Adoptive cell therapy (ACT) with tumor-infiltrating lymphocytes (TILs) is a promising immunotherapeutic approach for patients advanced solid tumors. While numerous advances have been made, the contribution of neoantigen-specific CD4 + T cells within TIL infusion products remains underexplored and therefore offers significant opportunity progress. Methods We analyzed infused from metastatic melanoma previously treated ACT presence cells. TILs were enriched on reactivity to...
RNA splicing is an important biological process associated with cancer initiation and progression. However, the contribution of alternative to pancreatic (PDAC) development not well understood. Here, we identify enrichment binding proteins (RBPs) involved in regulation linked PDAC progression from a forward genetic screen using Sleeping Beauty insertional mutagenesis mouse model cancer. We demonstrate downregulation RBFOX2, RBP FOX family, promotes liver metastasis. Specifically, show RBFOX2...
Abstract Background Breast cancer ranks first among malignant tumors, of which triple-negative breast (TNBC) is characterized by its highly invasive behavior and the worst prognosis. Timely diagnosis precise treatment TNBC are substantially challenging. Abnormal tumor vessels play a crucial role in progression treatment. Nitric oxide (NO) regulates angiogenesis maintains vascular homeostasis, while effective NO delivery can normalize vasculature. Accordingly, we have proposed here...
Spatial transcriptomics (ST) assays represent a revolution in how the architecture of tissues is studied by allowing for exploration cells their spatial context. A common element analysis delineating tissue domains or "niches" followed detecting differentially expressed genes to infer biological identity cell types. However, many studies approach differential expression using statistical approaches often applied non-spatial scRNA data (e.g., two-sample t-tests, Wilcoxon's rank sum test),...
The immunoregulatory cytokine macrophage migration inhibitory factor (MIF) is encoded in a functionally polymorphic locus that linked to the susceptibility of autoimmune and infectious diseases. MIF promoter contains 4-nucleotide microsatellite polymorphism (-794 CATT) repeats 5 8 times locus, with greater numbers associated higher mRNA levels. Because there no information about transcriptional regulation these common alleles, we used oligonucleotide affinity chromatography liquid...
Advances in medical technology have allowed for customized prognosis, diagnosis, and treatment regimens that utilize multiple heterogeneous data sources. Multiple kernel learning (MKL) is well suited the integration of high throughput MKL remains to be under-utilized by genomic researchers partly due lack unified guidelines its use, benchmark datasets. We provide three implementations R. These methods are applied simulated illustrate can select appropriate models. also apply combine clinical...