A5010522343- Chemical Synthesis and Analysis
- Metabolism, Diabetes, and Cancer
- Click Chemistry and Applications
- Liver Disease Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Pharmacology and Obesity Treatment
- Adrenal Hormones and Disorders
- Nitric Oxide and Endothelin Effects
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Regulation of Appetite and Obesity
- Diet and metabolism studies
- Cancer-related gene regulation
- Venous Thromboembolism Diagnosis and Management
- Diabetes Treatment and Management
- Cancer, Stress, Anesthesia, and Immune Response
- Vitamin K Research Studies
- Cardiovascular and Diving-Related Complications
- Cannabis and Cannabinoid Research
- Cardiac, Anesthesia and Surgical Outcomes
- Enhanced Recovery After Surgery
- Tea Polyphenols and Effects
- Acute Kidney Injury Research
- Amino Acid Enzymes and Metabolism
- Mental Health Research Topics
Wroclaw Medical University
2020-2024
Wrocław University of Science and Technology
2017-2023
AGH University of Krakow
2017-2023
Central Clinical Hospital
2020
Ministry of Interior and Administration
2020
There is a pressing need for molecular targets and biomarkers in gastric cancer (GC). We aimed at identifying aberrations L-arginine metabolism with therapeutic diagnostic potential. Systemic metabolites were quantified using mass spectrometry 293 individuals enzymes’ gene expression was 29 paired tumor-normal samples qPCR referred to pathology landscape. Patients or benign disorders had reduced systemic arginine, citrulline, ornithine elevated symmetric dimethylarginine dimethylamine....
Checkpoint inhibitors are a modern therapeutic approach for treating various types of cancer, metabolic diseases, and chronic infections. The main goal this therapy is to specifically unlock the immune system, allowing it recognize eliminate cancer cells or pathogens, primarily through activation T lymphocytes. Monoclonal antibodies used in treatment cancers, such as pembrolizumab (Keytruda), nivolumab (Opdivo), ipilimumab (Yervoy), carry several limitations, due their large molecular size....
L-arginine/nitric oxide pathway metabolites are altered in colorectal cancer (CRC). We evaluated underlying changes enzymes 55 paired tumor/tumor-adjacent samples and 20 normal mucosa using quantitative-PCR assessed the impact of classic novel oxicam analogues on enzyme expression intracellular metabolite concentration (LC-MS/MS) Caco-2, HCT116, HT-29 cells. Compared to mucosa, ARG1, PRMT1, PRMT5 were overexpressed both tumor tumor-adjacent tissue DDAH2 solely tissue. Tumor-adjacent had...
Gastric (GC) and esophageal (EC) cancers are highly lethal. Better understanding of molecular abnormalities is needed for new therapeutic targets biomarkers to be found. Expression 18 cancer-related genes in 31 paired normal-tumor samples was quantified by reversely-transcribed quantitative polymerase chain reaction (RTqPCR) systemic concentration 27 cytokines/chemokines/growth factors 195 individuals determined using Luminex xMAP technology. Only Ki67, CLDN2, BCLxL were altered GC while...
Immunosuppressive interleukins (IL)-4 and 13 may directly promote cancer but neither their status nor role in gastrointestinal tract is clarified. We aim at quantifying ILs receptors paired normal-tumor samples (n = 49/51) sera 263), using immunoassays RTqPCR, screening for effect on colonic cells. Both were elevated locally protein level all cancers only IL13 transcripts colon upregulated. Interleukin receptor expression reflected pathology to varying degrees, with the association...
Abstract Peptide foldamers containing both cis ‐β‐aminocyclopentanecarboxylic acid and α‐amino residues combined in various sequence patterns (ααβ, αααβ, αβααβ, ααβαααβ) were screened using CD NMR spectroscopy for the tendency to form helices. ααβ‐Peptides found fold into an unprecedented well‐defined 16/17/15/18/14/17‐helix. By extending length of or shifting a fragment from one terminus another ααβ‐peptides, balance between left‐handed right‐handed helix populations present solution can be...
Abstract This prospective study aimed to analyze whether the patients with pre-diabetes (pre-DM) reach TC (therapeutic concentration) of metformin during repeated, low, constant drug dose. The guidelines do not recommend any dose for this group patients. Based on previous after a 1700 mg/day seem therapeutic concentration, which guarantees glycemic effect. Twenty new-diagnosed pre-DM were treated 1500 regimen 15 weeks. serum concentration was assessed by liquid chromatography-mass...
A possibility of repurposing sitagliptin, a well-established antidiabetic drug, for alleviating injury caused by ischemia-reperfusion (IR) is being researched. The aim this study was to shed some light on the molecular background protective activity sitagliptin during hepatic IR. expression and/or concentration inflammation and oxidative stress-involved factors have been determined in rat liver homogenates using quantitative RT-PCR Luminex® xMAP® technology markers nitrative halogenative...
A new miniprotein built from three helices, including one structure based on the ααβαααβ sequence pattern was developed. Its crystal revealed a compact conformation with well-packed hydrophobic core of unprecedented structure. The formed dimers that were stabilized by interaction their surfaces.
Nanosecond pulsed electric field (nsPEF) has emerged as a promising approach for inducing cell death in melanoma, either standalone treatment or combination with chemotherapeutics. However, to date, there been shortage of studies exploring the impact nsPEF on expression cancer-specific molecules. In this investigation, we sought assess effects melanoma-specific MAGE (Melanoma Antigen Gene Protein Family) expression. To achieve this, melanoma cells were exposed parameters set at 8 kV/cm, 200...
Quantification with satisfactory specificity and sensitivity of free 3-Nitro-l-tyrosine (3-NT), 3-Chloro-l-tyrosine (3-CT), 3-Bromo-l-tyrosine (3-BT) in biological samples as potential inflammation, oxidative stress, cancer biomarkers is analytically challenging. We aimed at developing a liquid chromatography–tandem mass spectrometry (LC-MS/MS)-based method for their simultaneous analysis without an extract purification step by solid-phase extraction. Validation the developed yielded...
Recent studies place great importance on Protein-Bound Uraemic Toxins (PBUT) in the context of etiopathogenesis chronic kidney disease-associated pruritus (CKD-aP). This study aimed to investigate possible contribution free and total Indoxyl Sulfate (IS) p-Cresol (PCS) cause CKD-aP. Group A included 64 patients maintenance haemodialysis (HD) with B 62 HD that did not report CKD-aP, group C 50 healthy controls. Pruritus severity was assessed using a Numerical Rating Scale (NRS). Moreover,...
The L-arginine/NO pathway holds promise as a source of potential therapy target and biomarker; yet, its status utility in esophageal squamous cell carcinoma (ESCC) is unclear. We aimed at quantifying metabolites sera from patients with ESCC (n = 61) benign conditions 62) using LC-QTOF-MS enzyme expression tumors matched noncancerous samples 40) real-time PCR reference to pathology circulating immune/inflammatory mediators, quantified Luminex xMAP technology. was associated elevated systemic...
The authors evaluated the impact of different dose metformin on NO (nitric oxide) production in subjects with pre-diabetes. metformin-naïve patients from one Diabetic Center newly diagnosed pre-diabetes, without cardio-vascular diseases, were randomized (based identification number, individual for each inhabitant country) treatment doses (group A 3 × 500 mg, group B 1000 mg) 12 weeks. Then, switched to last wide panel L-arginine/NO pathway metabolites concentrations was assessed using liquid...
Antiplatelet therapy has become a standard therapeutic approach in the secondary prevention of cardiovascular system disorders thrombotic origin. Patients with concomitant diabetes mellitus (DM) obtain fewer benefits from this treatment. Hence, pathophysiology altered platelet function response to glucose metabolism impairment should be particular interest.The aim our study was verify if expression asymmetric dimethylarginine (ADMA) diabetic patients differs comparison nondiabetic ones. The...
Despite improvement in the management of modifiable cardiovascular risk factors, ischemic stroke remains leading cause morbidity and mortality adult population. The aim this study was to analyze time-dependent dynamic differences expression nitric oxide (NO) metabolic pathway platelet plasma compartment between subjects with without stroke. Additionally, interplay these parameters aggregation investigated. A total 418 patients acute phase non-cardioembolic were Following inclusion exclusion...
Excessive endocrine response to trauma negatively affects patients’ well-being. Cortisol dynamics following robot-assisted colorectal surgery are unknown. We aimed at determining the impact of cancer pathology and surgery-related factors on baseline cortisol levels analyzed its time-profile in patients undergoing open or surgery. were measured using liquid chromatography quadrupole time-of-flight mass spectrometry. Baseline was not associated with any patient- disease-related factors....
(1) Background: Type-2-diabetes-mellitus (DM) is one the most important cardiovascular-risk-factors. Among many molecules regulating vascular tone, nitric oxide appears to be pivotal. Although micro- and macrovascular-abnormalities are extensively studied, alterations in nitric-oxide-metabolic-pathway require further investigations. Additionally, role of erythrocytes tone regulation has not been explored. The aim this study was evaluate endothelial-function plasma diabetic individuals. (2)...
Molecular mechanisms underlying the beneficial effect of sitagliptin repurposed for hepatic ischemia-reperfusion injury (IRI) are poorly understood. We aimed to evaluate impact IRI and on profile eicosanoids (LC-MS/MS) expression/concentration (RTqPCR/ELISA) GLP-1/GLP-1R, SDF-1α/CXCR4 VIP/VPAC1, VPAC2, PAC1 in 36 rats. Animals were divided into four groups subjected ischemia (60 min) reperfusion (24 h) with or without pretreatment (5 mg/kg) (IR SIR) sham-operated (controls sitagliptin)....
Abstract Peptide foldamers containing both cis ‐β‐aminocyclopentanecarboxylic acid and α‐amino residues combined in various sequence patterns (ααβ, αααβ, αβααβ, ααβαααβ) were screened using CD NMR spectroscopy for the tendency to form helices. ααβ‐Peptides found fold into an unprecedented well‐defined 16/17/15/18/14/17‐helix. By extending length of or shifting a fragment from one terminus another ααβ‐peptides, balance between left‐handed right‐handed helix populations present solution can be...
The aim of this research was to design and synthesize new lipid conjugates 7-DHC that could serve as a storage form esterified provitamin D
BACKGROUND Acute kidney injury (AKI) is one of the most common organ failures. An early diagnosis AKI using specific biomarkers essential for effective treatment. This study determined serum concentrations selected amino acids and amines targeted liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in patients during sepsis septic shock treated Intensive Care Unit (ICU). MATERIAL AND METHODS A sample 41 was divided into 2 groups: (1) along required continuous renal...