A5072438384- Biopolymer Synthesis and Applications
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Polyamine Metabolism and Applications
- Ruminant Nutrition and Digestive Physiology
- Microbial Inactivation Methods
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Bacillus and Francisella bacterial research
- Cancer Research and Treatments
- Reproductive Physiology in Livestock
- Bacterial Genetics and Biotechnology
- Amino Acid Enzymes and Metabolism
- Listeria monocytogenes in Food Safety
- Genetic and phenotypic traits in livestock
- Radioactive contamination and transfer
- Cellular transport and secretion
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Anaerobic Digestion and Biogas Production
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Plant and animal studies
- Chemical Synthesis and Characterization
National Agriculture and Food Research Organization
2012-2022
Food Research Institute
2011-2021
Agriculture and Food
2018
Food Research Institute
2002-2017
Kanazawa University
1999-2013
Yakult Central Institute
2003-2007
National Center For Child Health and Development
2006
Ministry of Agriculture Forestry and Fisheries
1998
RIKEN
1998
University of Fukui
1994
Expression of vascular endothelial growth factor (VEGF)-C and that its receptors were assessed in non-small cell lung cancer. Immunohistochemistry revealed positive VEGF-C expression 38.7% (24/62) the patients studied. A significant correlation was found between cancer cells VEGF receptor-3 (VEGFR-3) cells, but not VEGFR-2 cells. In this cohort patients, significantly associated with lymph node metastasis, lymphatic vessel invasion, worse outcomes after operation. Although independent...
S100A4 has been implicated in the malignant phenotype of tumor cells, including cell motility, but biological function is hardly known. A recent study suggests that S100A4-induced invasiveness cells partially caused by down-regulation E-cadherin. To clarify clinical significance and its association with E-cadherin-mediated cell-to-cell adhesion system, we examined their protein expressions non-small lung cancer (NSCLC) specimens using immunohistochemical techniques. Expression was observed...
Spore-forming Bacillus strains that produce extracellular poly-γ-glutamic acid were screened for their application to natto (fermented soybean food) fermentation. Among the 424 strains, including subtilis and B. amyloliquefaciens, which we isolated from rice straw, 59 capable of fermenting natto. Biotin auxotrophism was tightly linked A multilocus nucleotide sequence six genes (rpoB, purH, gyrA, groEL, polC, 16S rRNA) used phylogenetic analysis, amplified fragment length polymorphism (AFLP)...
During early stationary phase, Bacillus subtilis NAFM5 produces capsular poly(gamma-glutamic acid) (gammaPGA, 2x10(6) Da), which contains D- and L-glutamate, then degrades it during late phase. The gamma-glutamyltransferase (EC 2.3.2.2; GGT) of this strain successively hydrolysed gammaPGA from the amino-terminal end, to yield both L-glutamate. This enzyme was specifically synthesized phase through transcriptional activation corresponding ggt gene by ComQXPA quorum-sensing system. A knockout...
ABSTRACT Some Bacillus subtilis strains, including natto (fermented soybeans) starter produce a capsular polypeptide of glutamate with γ-linkage, called poly-γ-glutamate (γ-PGA). We identified and purified monomeric 25-kDa degradation enzyme for γ-PGA (designated hydrolase, PghP) from bacteriophage ΦNIT1 in B. host cells. The PghP internally hydrolyzed to oligopeptides, which were then specifically converted tri-, tetra-, penta-γ-glutamates. Monoiodoacetate EDTA both inhibited the activity,...
Bacillus subtilis is the main component in fermentation of soybeans. To investigate genetics soybean-fermenting B. strains and its relationship with productivity extracellular poly-γ-glutamic acid (γPGA), we sequenced whole genome eight stains isolated from non-salted fermented soybean foods Southeast Asia. Assembled nucleotide sequences were compared those a natto (fermented food) starter strain BEST195 laboratory standard 168 that incapable γPGA production. Detected variants investigated...
Sec12p is the guanine nucleotide exchange factor of Sar1 GTPase and functions at very upstream in vesicle budding reactions from endoplasmic reticulum (ER). We previously identified three yeast loci, RST1, RST2, RST3, whose mutations suppressed temperature-sensitive growth sec12-4 mutant (Nakano, A. (1996) J. Biochem. (Tokyo) 120, 642-646). In present study, we cloned wild-type RST2 gene by complementation cold-sensitive phenotype rst2-1 mutant. turned out to be identical HRR25, a encoding...
The degQ gene of Bacillus subtilis (natto), encoding a small peptide 46 amino acids, is essential for the synthesis extracellular poly-gamma-glutamate (γPGA). To elucidate role DegQ in γPGA synthesis, we knocked out (natto) and screened suppressor mutations that restored absence DegQ. Suppressor were found degS, receptor kinase DegS-DegU two-component system. Recombinant DegS-His(6) mutant proteins expressed Escherichia coli cells subjected to an vitro phosphorylation assay. Compared with...
The pancreas and hypothalamus are critical for maintaining nutrient energy homeostasis, combined disorders in these organs account the onset of metabolic syndrome. Activating transcription factor 3 (ATF3) is an adaptive response factor. physiological role ATF3 has been controversial, its remains unknown. To elucidate roles organs, we generated pancreas- hypothalamus-specific Atf3 knockout (PHT-Atf3-KO) mice this study.We crossed bearing floxed alleles with Pdx1-cre mice, which cre...
Many bacteria, including Escherichia coli , have a unique gene that encodes glutamate racemase. This enzyme catalyses the formation of d -glutamate, which is necessary for cell wall peptidoglycan synthesis. However, Bacillus subtilis has two racemase genes, named racE and yrpC . Since appears to be indispensable growth in rich medium, role -amino acid synthesis vague. Experiments with - -knockout mutants confirmed essential medium but showed this was dispensable minimal where executes...
Pseudomonas aeruginosa PAO1 has two possible catabolic pathways of spermidine and spermine; one includes the spuA spuB products with unknown functions other involves dehydrogenase (SpdH; EC 1.5.99.6) encoded by an gene. The properties SpdH in P. were characterized corresponding spdH gene this strain identified. deduced (620 residues, calculated Mr 68,861) had a signal sequence 28 amino acids at terminal potential transmembrane segment between residues 76 92, accordance membrane location...
An industrial strain of Bacillus subtilis (natto) was used to produce poly-gamma-dl-glutamate (γPGA), a polymer dl-glutamate linked by γ-peptide bond. In spite efforts improve γPGA production modifying the medium, little attention has been paid expression synthetase gene. this study, we investigated synthetic gene and product under various conditions with LacZ-fusion (pgsB-lacZ). The 5′ upstream regulatory region pgsB also constructing deletion mutations lacZ-fusion. pgsB-lacZ clearly...
Abatement of greenhouse gas emitted from ruminants and promotion biogas energy animal effluent were comprehensively examined in each anaerobic fermentation reactor experiments.Moreover, the conversion efficiency biomass to power generation evaluated with a engine generator or proton exchange membrane fuel cell (PEMFC).To mitigate safely rumen methanogenesis nutritional manipulation suppressing effects some strains lactic acid bacteria yeast, bacteriocin, β1-4 galactooligosaccharide, plant...