A5044048823- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy and Chemometric Analyses
- Photoacoustic and Ultrasonic Imaging
- Gold and Silver Nanoparticles Synthesis and Applications
- Optical Coherence Tomography Applications
- Image Processing Techniques and Applications
- Near-Field Optical Microscopy
- Advanced biosensing and bioanalysis techniques
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Molecular Biology Techniques and Applications
- Advanced Fiber Laser Technologies
- Mass Spectrometry Techniques and Applications
- Chemical Reactions and Isotopes
- Lipid Membrane Structure and Behavior
- Organoselenium and organotellurium chemistry
- Optical Imaging and Spectroscopy Techniques
- Asymmetric Hydrogenation and Catalysis
- Digital Holography and Microscopy
- Click Chemistry and Applications
- Advanced Optimization Algorithms Research
- Optimization and Variational Analysis
- Photonic and Optical Devices
- Integrated Circuits and Semiconductor Failure Analysis
The University of Tokyo
2018-2024
Tokyo University of Information Sciences
2018-2023
Japan Science and Technology Agency
2021
Imaging of biological cells and tissues with subcellular spatial resolution is important in biology medicine because it allows us to explore the dynamics diagnose structure tissues. Among various optical imaging modalities, laser microscopy fluorescent staining a powerful method for this purpose. However, still suffers from limited applicability cytotoxicity process. Stimulated Raman scattering (SRS) an emerging technique based on molecular vibrational contrast, offering new opportunities...
Raman probes based on alkyne or nitrile tags hold promise for highly multiplexed imaging. However, sensing of enzyme activities with is difficult because few mechanisms are available to modulate the vibrational response. Here we present a general strategy prepare activatable that show enhanced signals due electronic preresonance (EPR) upon reaction enzymes under physiological conditions. We identified xanthene derivative bearing group at position 9 (9CN-JCP) as suitable scaffold dye, and...
Observing multiple molecular species simultaneously with high spatiotemporal resolution is crucial for comprehensive understanding of complex, dynamic, and heterogeneous biological systems. The recently reported super-multiplex optical imaging breaks the "color barrier" fluorescence to achieve multiplexing number over six in living systems, while its temporal limited several minutes mainly by slow color tuning. Herein, we report integrated stimulated Raman microscopy simultaneous multimodal...
Super-resolution vibrational microscopy is promising to increase the degree of multiplexing nanometer-scale biological imaging because narrower spectral linewidth molecular vibration compared fluorescence. However, current techniques super-resolution suffer from various limitations including need for cell fixation, high power loading, or complicated detection schemes. Here, we present reversible saturable optical Raman transitions (RESORT) microscopy, which overcomes these by using...
Detecting multiple enzyme activities simultaneously with high spatial specificity is a promising strategy to investigate complex biological phenomena, and Raman imaging would be an excellent tool for this purpose due its multiplexing capabilities. We previously developed activatable probes based on 9CN-pyronins, but specific visualization of cells target proved difficult leakage the hydrolysis products from after activation. Here, focusing rhodol bearing nitrile group at position 9...
Photoswitchable fluorescence is a powerful technique to realize super-resolution imaging, highlighting, and optical storage, while its multiplexing capability limited. Raman scattering attracting attention because it generates narrowband vibrational signatures, which are potentially useful for highly multiplexed detection of different constituents. Here, we demonstrate photoswitchable stimulated (SRS) spectroscopy microscopy where signatures switched with full reversibility at high speed....
9-cyanopyronin is a promising scaffold that exploits resonance Raman enhancement to enable sensitive, highly multiplexed biological imaging. Here, we developed cyano-Hydrol Green (CN-HG) derivatives as scaffolds expand the color palette of 9-cyanopyronins. CN-HG exhibit sufficiently long wavelength absorption produce strong for near-infrared (NIR) excitation, and their nitrile peaks are shifted lower frequency than those The fluorescence strongly quenched due lack 10th atom, unlike pyronin...
Plants can rapidly respond to different stresses by activating multiple signaling and defense pathways. The ability directly visualize quantify these pathways in real time using bioorthogonal probes would have practical applications, including characterizing plant responses both abiotic biotic stress. Fluorescence-based labels are widely used for tagging of small biomolecules but relatively bulky with potential effects on their endogenous localization metabolism. This work describes the use...
Polarized Raman spectroscopy and microscopy are known to enable the investigation of symmetry orientation molecular vibrational modes give additional spectroscopic signature. However, conventional always requires prolonged exposure ensure satisfying signal-to-noise ratio, which impedes fast imaging. Here, we demonstrate dual-polarization hyperspectral stimulated scattering with simultaneous accessibility two polarized images in orthogonal polarization states without modulation. Each pair...
Low-loss and broadband optics is important in optical measurement imaging. Here, we design an axicon-based beam shaper for low-loss microscopic by numerically calculating the transmittance focusing performance, assuming application to stimulated Raman scattering (SRS) microscopy. Furthermore, demonstrate equipped SRS microscope confirm that increases without sacrificing spatial resolution or signal intensity.
The dopaminergic system is essential for the function of brain in health and disease. Therefore, detailed studies focused on unraveling mechanisms involved signaling are required. However, lack probes that mimic dopamine living tissues, owing to neurotransmitter's small size, has hampered analysis system. current study aimed overcome this limitation by developing alkyne-tagged compounds (ATDAs) have a minimally invasive uniquely identifiable alkyne group as tag. ATDAs were established...
We present a widely wavelength-tunable fiber-based optical parametric oscillator (FOPO) with fixed repetition rate using four-wave mixing in photonic crystal fiber and an intracavity spectral filter. The FOPO produces nearly transform-limited pulses width of 1.1 nm pulse duration 1.0 ps. By changing the wavelength pump laser passband filter, tuning range between 832 922 is achieved. Over entire range, displacement delay stage required to match cavity round trip time period as small 0.8 mm....
Photoactivatable fluorescence probes can track the dynamics of specific cells or biomolecules with high spatiotemporal resolution, but their broad absorption and emission peaks limit number wavelength windows that be employed simultaneously. In contrast, narrower peak width Raman signals offers more scope for simultaneous discrimination multiple targets, therefore a palette photoactivatable would enable comprehensive investigation biological phenomena. Herein we report...
Abstract Alkyltrimethylammonium chlorides (ATACs) are widely utilized as cationic surfactants in various scientific fields. Furthermore, deuterium‐incorporated compounds can be powerful tools for elucidating the complicated morphologies of molecules with high molecular weights. Dodecyltrimethylammonium chloride (DTAC), a representative ATAC, underwent regioselective multi‐deuteration alkylamine moiety, except α and β ‐positions. Deuteration was performed under Pt/C Ru/C‐catalyzed conditions...
Polarization-resolved stimulated Raman scattering spectroscopies and microscopies have been utilized to investigate the symmetry orientation of molecular vibrational modes provide extra spectral signatures, while polarization modulation introduced additional complexity successive measurement on different states limits imaging speed. Here we demonstrate dual-polarization hyperspectral microscopy which enables detailed in two orthogonal simultaneously at video-rate Two polarized images can be...
Super-resolution vibrational microscopy holds great promise for enhancing the multiplexing capabilities of nanometer-scale biological imaging due to narrower spectral linewidth molecular vibrations compared fluorescence. However, current super-resolution techniques are plagued by several limitations, such as requirement cell fixation, high power consumption, or complex detection setups. Here we describe our recent demonstration reversible saturable optical Raman transitions (RESORT)...
We demonstrate a multispectral and multimodal microscopy which enables high-speed stimulated Raman fluorescence imaging. Both the wavenumber detection wavelength of each frame can be tuned via galvanometer-driven optical filters.
Among various optical methods, fluorescence imaging has been the most widely exploited thanks to its superior sensitivity and specificity, but resolvable colors are restricted 2-5 because of intrinsically broad featureless spectra. Recently, this fluorescent "color barrier" was broken super-multiplex became possible taking advantage well-designed Raman probes. However, acquisition images is still relatively slow which impedes wider applications. Here, we demonstrate fast organelle with...
Super-resolution vibrational microscopy is a promising tool to increase the degree of multiplexing nanometer-scale biological imaging, because spectral linewidth molecular vibration about 50 times narrower than that fluorescence. However, current techniques super-resolution still suffer from various limitations including need for cell fixation, high power loading or complicated frequency-modulated detection schemes. Herein we utilize photoswitchable stimulated Raman scattering (SRS) develop...
We present a fiber optical parametric oscillator incorporating wavelength-tunable pump pulses and an intracavity filter. A wide tuning range of 90 nm is achieved while keeping the repetition rate constant.
We present an environmentally stable, high-speed wavelength-tunable laser system with a tunability of >280 cm−1. This is synchronized to picosecond Ti:sapphire demonstrate video-rate hyperspectral stimulated Raman imaging polymer beads.