A5044106357- Pancreatic function and diabetes
- Protein Structure and Dynamics
- Neurobiology and Insect Physiology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Carbohydrate Chemistry and Synthesis
- Physiological and biochemical adaptations
- Enzyme Structure and Function
- Insect Resistance and Genetics
- Proteoglycans and glycosaminoglycans research
- Genetics, Aging, and Longevity in Model Organisms
- Metabolism, Diabetes, and Cancer
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Glycosylation and Glycoproteins Research
- Growth Hormone and Insulin-like Growth Factors
- Educational and Social Studies
- Insect Utilization and Effects
- Wheat and Barley Genetics and Pathology
- Plant biochemistry and biosynthesis
- Sperm and Testicular Function
- Food composition and properties
- Crystallization and Solubility Studies
- Diabetes Management and Research
- Williams Syndrome Research
Hull York Medical School
2024
University of York
2015-2024
European Molecular Biology Laboratory
2009-2015
Université Joseph Fourier
2009-2011
Université Grenoble Alpes
2011
Centre National de la Recherche Scientifique
2009-2011
Unit of Virus Host Cell Interactions
2009-2011
University of Toronto
2003
Abstract General transcription factor TFIID is a cornerstone of RNA polymerase II initiation in eukaryotic cells. How human TFIID—a megadalton-sized multiprotein complex composed the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs)—assembles into functional poorly understood. Here we describe heterotrimeric subcomplex consisting TAF2, TAF8 TAF10 proteins, which assembles cytoplasm. Using native mass spectrometry, define interactions between TAFs uncover central role for...
Fertilization involves the recognition and fusion of sperm egg to form a previously unidentified organism. In mammals, surface molecules on have central roles, while adhesion is mediated by IZUMO1-JUNO sperm-egg ligand-receptor pair, molecule/s responsible for membrane remain mysterious. Recently, MAIA/FCRL3 was identified as mammalian receptor, which bound IZUMO1 JUNO might therefore bridging role in gamete fusion. Here, we use sensitive assays designed detect extracellular protein binding...
Abstract The insulin-related hormones regulate key life processes in Metazoa, from metabolism to growth, lifespan and aging, through an evolutionarily conserved insulin signalling axis (IIS). In humans the IIS is controlled by insulin, two insulin-like growth factors, isoforms of receptor (hIR-A -B), its homologous IGF-1R. Drosophila , this engages seven (DILP1-7) a single (dmIR). This report describes cryoEM structure dmIR ectodomain:DILP5 complex, revealing high structural homology between...
The oligomeric state of the storage form human insulin in pancreas, which may be affected by several endogenous components β-cell granules such as arginine, is not known. Here, effect arginine on oligomerization investigated independently protein crystallography, molecular dynamics simulations, and capillary electrophoresis. combined results point to a strong ionic strength assembly. Molecular simulations electrophoretic measurements at low/mM salt concentrations show no significant...
Abstract The insulin/insulin-like growth factor signalling axis is an evolutionary ancient and highly conserved hormonal system involved in the regulation of metabolism, lifespan animals. Human insulin stored pancreas, while insulin-like factor-1 (IGF-1) maintained blood complexes with IGF-binding proteins (IGFBP1–6). Insect polypeptide binding (IBPs) have been considered as IGFBP-like structural functional homologues. Here, we report structures Drosophila IBP Imp-L2 its free form bound to...
We are witnessing tremendous advances in our understanding of the organization life. Complete genomes being deciphered with ever increasing speed and accuracy, thereby setting stage for addressing entire gene product repertoire cells, towards whole biological systems. Advances bioinformatics mass spectrometric techniques have revealed multitude interactions present proteome. Multiprotein complexes emerging as a paramount cornerstone activity, many proteins appear to participate, stably or...
Chorismate synthase (CS) (EC 4.6.1.4) catalyzes the last enzymatic step of shikimate biosynthetic pathway involving conversion 5-enolpyruvylshikimate 3-phosphate (EPSP) to chorismate.1 serves as a precursor for biosynthesis large number aromatic compounds, including amino acids, and secondary metabolites in plants. This is essential microbes, fungi, parasites plants, absent mammals, which makes it an excellent target design drug compounds herbicides. The potential such has been exemplified...
Structural details of changes accompanying interaction between insulin-related hormones and their binding partners are often enigmatic. Here, cross-linking/mass spectrometry could complement structural techniques reveal these protein-protein interfaces. We used such approach to clarify missing description the interface in human insulin-like growth factor (IGF-1):Drosophila melanogaster imaginal morphogenesis protein-late 2 protein (Imp-L2) complex which we studied previously by X-ray...
Abstract The family of insulin-related hormones regulates key life processes in Metazoa, from metabolism to growth, reproduction, lifespan and aging, through an evolutionarily conserved Insulin/insulin-like growth factor-1 (IGF-1) signalling (IIS) axis. In humans the IIS axis is controlled by insulin, two insulin-like factors, isoforms insulin receptor (hIR-A -B), its homologous IGF-1R their hybrid dimers. Drosophila , this engages seven proteins (DILP1-7) a single (dmIR), with many...
<div> <p>The oligomeric state of the storage form human insulin in pancreas, which may be affected by several endogenous components beta-cells granules such as arginine, is not known. Here, effect arginine on oligomerisation investigated independently protein crystallography, molecular dynamics simulations and capillary electrophoresis. The combined results point to a strong ionic strength assembly. Molecular electrophoretic measurements at low/mM salt concentrations show no...
ABSTRACT The insulin-related hormones regulate key life processes in Metazoa, from metabolism to growth, lifespan and aging, through an evolutionarily conserved insulin signalling axis (IIS). In humans the IIS is controlled by insulin, two insulin-like growth factors, isoforms of receptor (hIR-A -B), its homologous IGF-1R. Drosophila , this engages seven (DILP1-7) a single (dmIR). This report describes cryoEM structure dmIR ectodomain:DILP5 complex, revealing high structural homology between...
The oligomeric state of the storage form human insulin in pancreas, which may be affected by several endogenous components beta-cells granules such as arginine, is not known. Here, effect arginine on oligomerisation investigated independently protein crystallography, molecular dynamics simulations and capillary electrophoresis. combined results point to a strong ionic strength assembly. Molecular electrophoretic measurements at low/mM salt concentrations show no significant aggregation. In...