A5032211571- Plant Molecular Biology Research
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Circular RNAs in diseases
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Soybean genetics and cultivation
- Plant Pathogenic Bacteria Studies
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Plant and Fungal Interactions Research
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogens and Resistance
Donald Danforth Plant Science Center
2018-2025
University of California, Davis
2024-2025
Center for Research in Agricultural Genomics
2014-2024
Universitat Autònoma de Barcelona
2014-2021
Consejo Superior de Investigaciones Científicas
2014
Universitat de Barcelona
2014
Abstract The OsCPK4 gene is a member of the complex family calcium-dependent protein kinases in rice (Oryza sativa). Here, we report that expression induced by high salinity, drought, and phytohormone abscisic acid. Moreover, plasma membrane localization was observed transient assays green fluorescent protein-tagged onion (Allium cepa) epidermal cells. Overexpression plants significantly enhances tolerance to salt drought stress. Knockdown plants, however, are severely impaired growth...
Small RNAs (sRNAs) that are 21 to 24 nucleotides (nt) in length found most eukaryotic organisms and regulate numerous biological functions, including transposon silencing, development, reproduction, stress responses, typically via control of the stability and/or translation target mRNAs. Major classes sRNAs plants include microRNAs (miRNAs) small interfering (siRNAs); known travel as a silencing signal from cell cell, root shoot, even between host pathogen. In mammals, transported inside...
Previously, we have shown that apoplastic wash fluid (AWF) purified from Arabidopsis leaves contains small RNAs (sRNAs). To investigate whether these sRNAs are encapsulated inside extracellular vesicles (EVs), treated EVs isolated with the protease trypsin and RNase A, which should degrade located outside but not those inside. These analyses revealed mostly associated proteins. Further of (exRNAs) they include both long noncoding (lncRNAs), including circular (circRNAs). We also found exRNAs...
Abstract MicroRNAs (miRNAs) play a pivotal role in regulating gene expression during plant development. Although substantial fraction of miRNAs has proven responsive to pathogen infection, their disease resistance remains largely unknown, especially fungal infections. In this study, we screened Arabidopsis thaliana lines which miRNA activity been reduced using artificial target mimics ( MIM lines) for response pathogens. Reduced miR396 MIM396 plants) was found confer broad necrotrophic and...
MicroRNAs (miRNAs) are small non-coding RNAs that have important regulatory functions in plant growth, development, and response to abiotic stress. Increasing evidence also supports miRNAs contribute immune responses pathogens. Here, we used deep sequencing of RNA libraries for global identification rice regulated by fungal elicitors. We describe 9 previously uncharacterized rice. Combined degradome analyses revealed networks enriched elicitor-regulated supported the their corresponding...
Summary MicroRNAs (miRNAs) are small non‐coding RNAs that direct post‐transcriptional gene silencing in plant development and stress responses through cleavage or translational repression of target mRNAs. Here, we report the identification functional characterization a new member miR812 family rice (named as miR812w) involved disease resistance. miR812w is present cultivated Oryza species, both japonica indica subspecies, wild species within genus, but not dicotyledonous species. 24nt‐long...
In Arabidopsis thaliana, ARGONAUTE1 (AGO1) plays a central role in microRNA (miRNA) and small interfering RNA (siRNA)-mediated silencing. AGO1 associates to the rough endoplasmic reticulum conduct miRNA-mediated translational repression, mRNA cleavage, biogenesis of phased siRNAs. Here, we show that 37°C heat stress (HS) promotes protein accumulation cytosolic condensates where it colocalizes with components siRNA bodies granules. contains prion-like domain its poorly characterized...
Transgenic expression of a double-stranded RNA in plants can induce silencing homologous mRNAs fungal pathogens. Although such host-induced gene is well documented, the molecular mechanisms by which RNAs move from cytoplasm plant cells across plasma membrane both host cell and are poorly understood. Indirect evidence suggests that this transfer may occur at very early stage infection process, prior to breach wall, suggesting might be secreted onto leaf surfaces. To assess whether Arabidopsis...
MicroRNAs (miRNAs) are short regulatory non-coding RNAs that guide gene silencing in most eukaryotes. They regulate expression by triggering sequence-specific cleavage or translational repression of target transcripts. Plant miRNAs known to play important roles a wide range developmental processes. Increasing evidence also supports the modulation miRNA levels plays an role reprogramming plant responses abiotic stress (drought, cold, salinity and nutrient deficiency) biotic (antibacterial...
MicroRNAs (miRNAs) are 21- to 24-nucleotide short noncoding RNAs that trigger gene silencing in eukaryotes. In plants, miRNAs play a crucial role wide range of developmental processes and adaptive responses abiotic biotic stresses. this work, we investigated the miR773 modulating resistance infection by fungal pathogens Arabidopsis thaliana. Interference with activity target mimics (in MIM773 plants) concomitant upregulation METHYLTRANSFERASE 2 (MET2) increased necrotrophic...
Abstract Aegilops tauschii is the donor of D subgenome hexaploid wheat and an important genetic resource. The reference-quality genome sequence Aet v4.0 for Ae. acc. AL8/78 was therefore milestone biology breeding. Further advances in sequencing release v5.0 assembly are reported here. Two new optical maps were constructed used revision pseudomolecules. Gaps closed with Pacific Biosciences long-read contigs, decreasing gap number by 38,899. Transposable elements protein-coding genes...
RNA silencing is a conserved mechanism in eukaryotes involved development and defense against viruses. In plants, ARGONAUTE1 (AGO1) protein plays central role both microRNA- small interfering RNA-directed silencing, its expression regulated at multiple levels. Here, we report that the F-box FBW2 assembles an SCF complex selectively targets for proteolysis AGO1 when it unloaded mutated. Although loss of function does not lead to strong growth or developmental defects, significantly increases...
Small RNAs (sRNAs), including small interfering (siRNAs) and microRNAs (miRNAs), have emerged as important regulators of eukaryotic gene expression. In plants, miRNAs play critical roles in development, nutrient homeostasis abiotic stress responses. Accumulating evidence also reveals that sRNAs are involved plant immunity. Most studies on pathogen-regulated been conducted Arabidopsis plants infected with the bacterial pathogen Pseudomonas syringae, or treated flagelin-derived elicitor...
Phased, secondary siRNAs (phasiRNAs) represent a class of small RNAs in plants generated via distinct biogenesis pathways, predominantly dependent on the activity 22-nt miRNAs. Most miRNAs are processed by DCL1 from miRNA precursors containing an asymmetric bulge, yielding 22/21-nt miRNA/miRNA* duplex. Here we show that miR1510, soybean capable triggering phasiRNA production numerous nucleotide-binding leucine-rich repeat (NB-LRRs), previously described as 21 nt its mature form, primarily...
Regulated gene expression is key to the orchestrated progression of cell cycle. Many genes are expressed at specific points in cycle, including important cycle regulators, plus factors involved signal transduction, hormonal regulation, and metabolic control. We demonstrate that post-embryonic depletion Arabidopsis (Arabidopsis thaliana) ARGONAUTE1 (AGO1), main effector plant microRNAs (miRNAs), impairs division root meristem. utilized highly synchronizable tobacco (Nicotiana tabacum) Bright...
PhasiRNAs (phased small interfering RNAs) are a major class of plant RNAs known to be key regulators in male reproductive development maize (Zea mays) and rice (Oryza sativa), among other plants. Earlier research focused primarily on premeiotic 21-nt phasiRNAs meiotic 24-nt phasiRNAs, while new studies uncovered phasiRNAs. The biogenesis function these remain unclear. We conducted an integrative analysis combining RNA (sRNA) sequencing transcriptomic profiling sRNA-associated genes across...
The Soybean-Bradyrhizobium symbiosis enables symbiotic nitrogen fixation (SNF) within root nodules, reducing reliance on synthetic N-fertilizers. However, is transient, peaking several weeks after Bradyrhizobium colonization and declining as nodules senesce in coordination with host development. To investigate the regulatory mechanisms governing SNF senescence, we conducted a temporal transcriptomic analysis of soybean inoculated diazoefficiens USDA110. Weekly nodule samples (2-10...
Maize somatic embryogenesis (SE) requires the induction of embryogenic callus and establishment proliferation before plant regeneration. The molecular mechanisms underlying potential are not well understood. Here we explored role small RNAs (sRNAs) accumulation their target transcripts in maize SE at dedifferentiation step using VS-535 zygotic embryos collected distinct developmental stages displaying contrasting vitro morphology. MicroRNAs (miRNAs), trans-acting siRNAs (tasiRNAs),...
SUMMARY Noncoding and coding RNAs are key regulators of plant growth, development, stress responses. To investigate the types transcripts accumulated during vegetative to reproductive transition floral development in Coffea arabica L., we sequenced small RNA libraries from eight developmental stages, up anthesis. We combined these data with messenger PARE sequencing two important stages that marks an apparent latent a rapid growth stage. In addition, took advantage multiple silico tools...
The Solanaceae or "nightshade" family is an economically important group with remarkable diversity. To gain a better understanding of how the unique biology relates to family's small RNA (sRNA) genomic landscape, we downloaded over 255 publicly available sRNA data sets that comprise 2.6 billion reads sequence data. We applied suite computational tools predict and annotate two major classes: (1) microRNAs (miRNAs), typically 20- 22-nucleotide (nt) RNAs generated from hairpin precursor...
Summary Localization of mRNA and small RNAs (sRNAs) is important for understanding their function. Fluorescent in situ hybridization (FISH) has been used extensively animal systems to study the localization expression sRNAs. However, current methods fluorescent detection sRNA plant tissues are less developed. Here we report a protocol (sRNA‐FISH) efficient sRNAs plants. This suitable application diverse species tissue types. The use locked nucleic acid probes antibodies conjugated with...
MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional regulators of gene expression via sequence-specific cleavage or translational repression target transcripts. They transcribed long single-stranded RNA precursors with unique stem–loop structures processed by a DICER-Like (DCL) ribonuclease, typically DCL1, to produce mature miRNAs. Although plethora miRNAs have been found be regulated pathogen infection in plants, the biological function most remains largely...