A5034211356- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Advancements in Transdermal Drug Delivery
- Animal Behavior and Welfare Studies
- Asthma and respiratory diseases
- Wound Healing and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Entomological Studies and Ecology
- Cytokine Signaling Pathways and Interactions
- Diabetes and associated disorders
- Food Allergy and Anaphylaxis Research
- SARS-CoV-2 and COVID-19 Research
- Contact Dermatitis and Allergies
- Veterinary Equine Medical Research
- Barrier Structure and Function Studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Neuroendocrine regulation and behavior
University of Florida
2020-2024
No study has directly compared the various treatment options for canine atopic dermatitis and their effects on skin barrier.To compare prednisone, oclacitinib, ciclosporin lokivetmab of dermatitis.Nineteen beagle dogs.Controlled, blinded study. Dogs were challenged with allergen twice weekly randomized to ciclosporin, lokivetmab, prednisone or no four weeks. Dermatitis pruritus assessed at baseline after each challenge. Transepidermal water loss (TEWL) hydration measured baseline, Day (D)14...
Filaggrin is important for the skin barrier and atopic dermatitis. Another filaggrin-like protein, filaggrin 2, has been described. We evaluated antibodies against both filaggrins in normal biopsies from dogs before after allergen challenges (D0, D1, D3 D10). Filaggrins expression was by immunohistochemistry Western blot. used PCR to investigate changes gene expression. Effects of group (
Introduction Canine atopic dermatitis (AD) closely mimics human AD and is recognized as a beneficial animal model. House dust mites (HDM) are common allergen for both species. The effects of chronic exposure to HDM on the skin have not been studied in this model, it known how changes gene expression correlate severity dermatitis. Methods We used an established canine model took biopsies before (D0) five times during repeated challenges (on Days 1, 2, 8, 15, 29, hereafter referred D1, D2, D8,...
Abstract OBJECTIVE This study investigated the effects of recombinant equine IL-31 (eIL-31) in vivo and vitro. METHODS Equine mRNA sequences were verified by sequencing. Recombinant eIL-31 was produced using mammalian bacterial expression systems. From November 2019 through February 2021, 12 normal horses, 6 to 10 years old with no history or clinical signs consistent allergic skin disease, injected ID saline 2 challenge studies. Pruritus-associated behaviors recorded for a minimum 15...
Skin barrier dysfunction is important in atopic dermatitis and can be secondary to inflammation. Observation of keratinocytes culture may show intrinsic differences. TransEpithelial Electrical Resistance (TEER) measures epithelial permeability. We cultured normal found that TEER was significantly lower (p < 0.0001) than normals. Atopic grew upwards, first creating isolated dome-like structures later horizontally into a monolayer. At time confluence (D0), were more differentiated, with higher...
Abstract This prospective, 4‐week, placebo‐controlled, cross‐over study aimed to investigate the efficacy of 1% topical κ‐opioid agonist, asimadoline, in a model canine atopic dermatitis (AD). Fourteen beagles were challenged with house dust mites every 3–4 days for total 9 challenges. Severity was assessed, and pruritus monitored using GoPro HERO cameras. Pruritus scoring evaluated at 10 time periods; baseline, 4 h post allergen challenge last day on Day 28. Scoring done blindly by...
Canine progenitor epidermal keratinocytes (CPEK) are used as canine keratinocyte cell line. Their suitability for skin barrier studies is unknown. Measurement of transepithelial electric resistance (TEER) evaluates epithelial permeability. We compared TEER and tight junction (TJ) expression in CPEKs normal (NK) harvested from biopsies dogs. NK were grown until confluence (D0) 13 additional days. Slides fixed on D0 stained with ZO-1 claudin-1 antibodies. Five images/antibody taken, randomized...
Preliminary evidence supports a role for IL-31 in equine insect bite hypersensitivity (IBH) and pruritus. Our studies investigated receptor-α (IL-31RA) transcription leukocytes from normal IBH horses response to Culicoides nubeculosus.19 15 were recruited the summer of 2019 (low-dose study) 8 10 winter 2022 2023 (high-dose study). Normal had no history or signs allergic skin disease, while clinical compatible with IBH. Pruritus was scored using visual analog score 1 6 grading system.Whole...
Janus kinase (JAK) pathways have emerged as targets of treatment, yet localization and expression JAK1 JAK3 in canine atopic skin not been studied. This study aimed to compare the dogs before after allergen exposure. Skin biopsies taken from beagles sensitized house dust mites (HDM) (D0) four weeks (D28) exposure were stained. Staining was subjectively scored by examiners unaware source slides. Image J used for semiquantitative assessment staining intensity. epidermal dermal. cytoplasmic,...
Objective: About 80% of corona virus disease-19 (COVID-19) deaths due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection occur in subject’s ≥ 65 years age, even though subjects this age group only account for about 10% COVID-19 cases. Our objectives were assess effects and the clinical utility of antibody levels health, disease, post-vaccination. Methods: We measured serum SARS-CoV-2 immunoglobulin M (IgM), IgG neutralizing antibodies using immunoassay kits...