A5058457879- Growth Hormone and Insulin-like Growth Factors
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Diabetes Management and Research
- Thyroid Disorders and Treatments
- Bone health and treatments
- Pituitary Gland Disorders and Treatments
- Pharmaceutical studies and practices
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Birth, Development, and Health
- Cellular transport and secretion
- Diet and metabolism studies
- Pharmacology and Obesity Treatment
- Bone health and osteoporosis research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Lipid metabolism and disorders
- Reproductive Biology and Fertility
- Folate and B Vitamins Research
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Rabbits: Nutrition, Reproduction, Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Pancreatic function and diabetes
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
Mbarara University of Science and Technology
2025
Makerere University Walter Reed Project
2021-2023
Mountains of the Moon University
2023
Novo Nordisk (United States)
2008-2019
Novo Nordisk (United Kingdom)
2015
Novo Nordisk (Denmark)
2013-2015
The University of Texas Southwestern Medical Center
2008
Harvard University
1986-2005
Joslin Diabetes Center
1985-2005
Beth Israel Deaconess Medical Center
1992-2005
It has been suggested that elevated levels of insulin or insulin-like growth factors (IGFs) play a role in the development diabetic vascular complications. Previously, we have shown differential response to between cells from retinal capillaries and large arteries with former being much more responsive. In present study, characterized receptors growth-promoting effect insulinlike factor I (IGF-I) multiplication-stimulating activity (MSA, an IGF-II) on endothelial pericytes calf smooth muscle...
Multiplication-stimulating activity (MSA), purified from medium conditioned by the BRL-3A rat liver cell line, previously has been shown to be closely related human somatomedins or insulin-like growth factors. A radioimmunoassay was utilized measure MSA levels in sera fetal, maternal, and young rats. serum somatomedin-binding protein found interfere competing with antibody for binding 125I-labeled MSA. Therefore, prior radioimmunoassay, were filtered on Sephadex G-75 1 M acetic acid...
Insulin and insulin-like growth factor type I (IGF-I) stimulate an overlapping spectrum of biological responses in human skin fibroblasts. Although insulin IGF-I are known to the incorporation [3H]thymidine into DNA these cells, identity receptor(s) that mediates this effect has not been fully clarified. The mouse anti-human receptor antibody alpha IR-3 binds with specificity but receptors placental membranes; it also specifically inhibits binding 125I-labeled suspensions fibroblasts a...
We demonstrate that therapeutically useful amounts of insulin are absorbed by the nasal mucosa human beings when administered as a spray with common bile salts. By employing series salts subtle differences in number, position, and orientation their nuclear hydroxyl functions alterations side chain conjugation, we show adjuvant potency for absorption correlates positively increasing hydrophobicity salts' steroid nucleus. As inferred from studies various concentrations unconjugated...
The expression of insulin-like growth factor (IGF) receptors at the cell surface and changes in IGF responsiveness during differentiation were studied L6 skeletal muscle line.Throughout entire developmental sequence, distinct for I I1 that differed structure peptide specificity could be demonstrated.During differentiation, both 12'I-IGF 1261-IGF binding to cells decreased as a result 3-4-fold reduction receptor number, whereas 1251-insulin increased.Under nonreducing conditions,...
Insulin-resistant hyperinsulinemic states are now widely known to be associated with ovarian hyperandrogenism,and this is thought due an action of insulinon the ovary. However, identity receptor that responsible for insulin in these patients, whose receptors on classical target tissues severely impaired, unclear. We report presence stromal and follicular compartments as well granulosa cells obtained from normal ovaries. After 15-h incubations at 4 C [125I]insulin tissue fragments, specific...
We have studied the effects of thyrotropin (TSH) on growth and levels mRNAs cellular proto-oncogenes, c-myc, c-fos, in specific target TSH action, thyroid follicular cell.FRTL5 cells, a cloned line from normal rat gland that depends upon for its replication, were maintained quiescent state 5 days by keeping them medium devoid serum or TSH.The addition bovine (bTSH, 1 nM) increased DNA synthesis stimulated cell proliferation after lag pe- riod 24 h.This response was anteceded prompt, but...
The strong association between hyperinsulinemic states of insulin resistance and ovarian hyperandrogenism has led to the suggestion that might directly influence function ovary. To assess this possibility, we have attempted measure receptors in stroma three patients who were operated upon for polycystic disease. 125I-insulin binding was easily detectable fragments each case. Specific totally inhibited by pre-treatment with serum containing specific anti-insulin receptor autoantibodies (B-2)....
Explants of 19- to 20-day fetal rat liver synthesize polypeptides biochemically and immunologically related the well characterized somatomedin (insulin-like growth factor) BRL-MSA, multiplication-stimulating activity. Fetal MSA was purified from media conditioned by explants chromatography on Sephadex G-75 under acid conditions. Partially MSA: 1) inhibited binding BRL-MSA receptor plasma membranes, somatomedin-binding proteins serum, rabbit anti-BRL-MSA serum; 2) had a molecular weight 4,500...
Insulin-like growth factors (IGFs) are potent mitogens for FRTL5 rat thyroid follicular cells. IGFs also synergize the independent mitogenic effects of thyrotropin-stimulating hormone (TSH) and other agents that increase intracellular AMP concentration. We examined whether cells M12 cells, a TSH-independent mutant cell line derived therefrom, secrete IGF regulates Immunoreactive IGF-II, but not IGF-I, was found in media conditioned by cells; from contained four- to fivefold higher...
Recombinant human (rh) insulin-like growth factor-I (IGF-I) is a potential therapy for individuals with severe insulin resistance, but its efficacy, mechanism of action, or duration effect these patients have not been explored fully. Two subjects the type A phenotype resistance without receptor mutations were investigated to assess secretion, and carbohydrate tolerance before after 3-4 weeks rhIGF-I treatment (100 micrograms/kg, sc, twice daily). Tests included 24-h glucose profile (modal...
We have performed clinical, physiological, in vitro biochemical and genetic studies of a patient with severe insulin resistance associated the phenotype "pseudoacromegaly," defined as presence acromegaloid features absence elevated levels GH or insulin-like growth factor-I (IGF-I). Despite marked hyperinsulinemia, IGF-I binding to circulating blood cells cultured skin fibroblasts was normal. Insulin IGF-I-stimulated autophosphorylation their respective receptors also However, neither nor...
In a family expressing euthyroid hyperthyroxinemia, an increased association of plasma thyroxine (T4) with transthyretin (TTR) is transmitted by autosomal dominant inheritance and secondary to mutant TTR molecule affinity for T4. Eight individuals spanning three generations exhibited the abnormality. Although five eight had elevated total T4 concentrations, all affected were clinically normal free levels. Purified from propositus 125I-T4 times that control TTR. Exons 2, 3, 4 (representing...
Aims To report preliminary data on baseline serum calcitonin concentrations and associated clinical characteristics in a global population with type 2 diabetes before liraglutide or placebo randomization. Methods The ongoing LEADER trial has enrolled 9340 people at high risk of cardiovascular disease 410 centres worldwide. People ≤50 ng/l were randomized to once daily will be followed for up 5 years. Serum was measured annually thereafter. An independent committee thyroid experts oversee...
Background Extended-spectrum β-lactamase (ESBL) producing bacteria pose a global challenge because of resistance developing against wide range antimicrobial agents that complicate available treatment options. Thus, identifying the prevalent bacterial species ESBL enzymes and understanding how they are susceptible to antibiotics is necessary internationally inform effective guidelines. Objective To determine prevalence molecular characterization causing Urinary Tract Infections (UTIs) in...
Allogeneic cortical bone screws offer a promising alternative to traditional metallic orthopedic screws, primarily due their potential for improved biocompatibility and osteointegration. However, earlier designs of these biological implants faced significant challenges, including antigenicity, inconsistent mechanical properties, limited bioactivity, inadequate fixation performance—factors that restricted clinical adoption. This study addresses issues through two key innovations: redesigned...
Bisphosphonates have been shown to decrease bone turnover in a variety of high states. We postulated that pamidronate (APD), bisphosphonate, could prevent the increased caused by thyroid hormone excess. Twenty-two male subjects were randomized receive either placebo (group 1) or APD (30 mg, iv, daily for 2 days; group 2). Subsequently, all received T3 (50 micrograms, twice daily, 8 days). Biochemical indices measured blood and urine at baseline, after treatment with APD/placebo, T3. The...
We designed a 2 × factorial, randomized, double-blinded, placebo-controlled trial to evaluate the effects of treatment with pioglitazone and/or fenofibrate in patients highly active antiretroviral therapy (HAART)–induced metabolic syndrome. found that administration pioglitazone, but not fenofibrate, improved insulin resistance, blood pressure, and lipid profile over 12-month period.