A5066781577- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Mass Spectrometry Techniques and Applications
- Probiotics and Fermented Foods
- Smart Materials for Construction
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Concrete Corrosion and Durability
- Plant tissue culture and regeneration
- Phytochemistry and Bioactive Compounds
- Tannin, Tannase and Anticancer Activities
- Microfluidic and Bio-sensing Technologies
- Phytochemical Studies and Bioactivities
- Bacterial Identification and Susceptibility Testing
- Plant biochemistry and biosynthesis
- Fiber-reinforced polymer composites
- Essential Oils and Antimicrobial Activity
- Microfluidic and Capillary Electrophoresis Applications
- Technology Adoption and User Behaviour
- Structural Behavior of Reinforced Concrete
- Metabolomics and Mass Spectrometry Studies
- ERP Systems Implementation and Impact
- Plant Virus Research Studies
- Natural product bioactivities and synthesis
Harbin Institute of Technology
2022-2023
Agricultural Biotechnology Research Center, Academia Sinica
2014-2022
Academia Sinica
2018
Taipei Medical University
2011
Potato common scab, which is caused by soil-borne Streptomyces species, a severe plant disease that results in significant reduction the economic value of potatoes worldwide. Due to lack efficacious pesticides, crop rotations, and resistant potato cultivars against disease, we investigated whether biological control can serve as an alternative approach. In this study, multiple Bacillus species were isolated from healthy tubers, amyloliquefaciens Ba01 was chosen for further analyses based on...
Abstract Rice bacterial blight caused by Xanthomonas oryzae pv. (Xoo ) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant cultivars, it desirable develop effective biocontrol agents against Xoo . Here, we report that a soil bacterium Pseudomonas taiwanensis displayed strong antagonistic activity Using matrix-assisted laser desorption/ionization imaging mass spectrometry, identified an iron chelator, pyoverdine, secreted P. could...
This study tried to clarify the antagonistic effect of lipopeptides secreted by Bacillus amyloliquefaciens strain BPD1 (Ba-BPD1) against Pyricularia oryzae Cavara (PO). To determine major antifungal effective PO, single and dual cultures were carried out in solid-state media. The matrix-assisted laser desorption/ionization-time flight imaging mass spectrometry (MALDI-TOF IMS) was used identify most lipopeptide situ. Meanwhile, morphology pathogen fungi treated with observed via SEM. Of three...
Two new steroidal saponins, 8 and 10, along with 7 known sapogenins saponins (1–7) a furostanol saponin (9) were isolated from Agave sisalana Perrine ex Engelm. The structures of these two compounds identified characterized by 1D 2D NMR spectroscopy mass spectrometry. In addition, acid hydrolysis GC‐FID used to confirm the sugar moieties 10. cytotoxic effects 1–10 on MCF-7, NCI-H460, SF-268 cancer cells evaluated, among them, compound 10 proved be most IC50 values 1.2, 3.8, 1.5 µM, respectively.
Abstract Bacterial polyynes are highly active natural products with a broad spectrum of antimicrobial activities. However, their detailed mechanism action remains unclear. By integrating comparative genomics, transcriptomics, functional genetics, and metabolomics analysis, we identified unique polyyne resistance gene, masL (encoding acetyl-CoA acetyltransferase), in the biosynthesis gene cluster antifungal (massilin A 1 , massilin B 2 collimonin C 3 D 4 ) Massilia sp. YMA4. Crystallographic...
Mass spectrometry (MS) interfacing technology provides the means for incorporating microfluidic processing with post MS analysis.
Plant viruses cause devastating diseases in plants, yet no effective viricide is available for agricultural application. We screened cultured filtrates derived from various soil microorganisms vegetable broth that enhanced plant viral resistance. A filtrate, designated F8 culture a fungus belonging to the genus Trichosporon, induced strong resistance on different plants. Our inoculation assay found infection rate of Tobacco mosaic virus (TMV)-inoculated Nicotiana benthamiana with filtrate...
Abstract Bacterial polyynes are highly active natural products with a broad-spectrum of antimicrobial activities. However, their detailed mechanism action remains unclear. Through integrating comparative genomics, transcriptomics, functional genetics, and metabolomics analysis, we identified unique polyyne resistance gene, masL (encoding acetyl-CoA acetyltransferase), from the biosynthesis gene cluster (BGC) dominant for production antifungal (massilin A, massilin B, collimonin C, D) in...
Abstract Bacterial polyynes are highly active natural products with a broad spectrum of antimicrobial activities. However, their detailed mechanism action remains unclear. By integrating comparative genomics, transcriptomics, functional genetics, and metabolomics analysis, we identified unique polyyne resistance gene, masL (encoding acetyl-CoA acetyltransferase), in the biosynthesis gene cluster antifungal (massilin A 1 , massilin B 2 collimonin C 3 D 4 ) Massilia sp. YMA4. Crystallographic...