A5024646746- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Valve Diseases and Treatments
- Public Health in Brazil
- Aortic aneurysm repair treatments
- Bone Tissue Engineering Materials
- Xenotransplantation and immune response
- Congenital Heart Disease Studies
- Cardiac Structural Anomalies and Repair
- Bone and Dental Protein Studies
- Reconstructive Surgery and Microvascular Techniques
- Cardiac and Coronary Surgery Techniques
- Medication Adherence and Compliance
- Corrosion Behavior and Inhibition
- Extracellular vesicles in disease
- Muscle and Compartmental Disorders
- Protease and Inhibitor Mechanisms
- Aortic Disease and Treatment Approaches
- Infective Endocarditis Diagnosis and Management
- Cardiovascular Conditions and Treatments
- Orthopaedic implants and arthroplasty
- Molecular Biology Techniques and Applications
- Mechanical Circulatory Support Devices
- Muscle Physiology and Disorders
- Collagen: Extraction and Characterization
Pontifícia Universidade Católica do Paraná
2010-2022
Universidade Federal do Paraná
2019
Universidade Tecnológica Federal do Paraná
2019
Centro Universitário Campos de Andrade
2014
Abstract Pericardial membrane derived from bovine heart tissues is a promising source of material for use in tissue‐engineering applications. However, tissue processing required its humans due to the presence animal antigens. Therefore, purpose this study was evaluate structural integrity and biocompatibility pericardium (BP) after soft decellularization process with 0.1% sodium dodecyl sulfate (SDS) solution, aim remove xenoantigens preserve extracellular matrix (ECM) bioactivity. The...
Decellularization is an alternative method for processing biological tissues with decreased antigenicity and resistance to calcification. The aim of this study was characterize the properties decellularized (dCell) bovine pericardium fixed 0.1% glutaraldehyde (GA) evaluate outcomes bioprosthetic valves constructed tissue when implanted in mitral position juvenile sheep.Bioprosthetic were fresh 0.5% GA (control group) or dCell (study group). Before implantation, samples submitted histological...
The cryopreserved homograft is a good valve substitute due attributes like excellent hemodynamics, low incidence of thromboembolic events, infection resistance and mid-term durability. However, progressive degeneration fibrocalcification may occur, particularly in the childhood young adults. Their antigenicity triggers an immunological reaction that plays important role their failure. decellularization process was proposed to decrease this antigenicity. By action detergents enzymes, removes...
Abstract Tissue‐engineered heart valves aim to reproduce the biological properties of natural with anatomically correct structure and physiological performance. The closest alternative creating an ideal valve substitute is use decellularized porcine valves, due their anatomy availability. However, immunological barrier structural maintenance limit long‐term performance valves. This study investigated extracellular matrix ( ECM ) aortic pulmonary by a low concentration sodium dodecyl sulfate...
OBJETIVO: Este estudo avalia o comportamento biológico dos heteroenxertos porcinos descelularizados (Grupo Desc) comparados com os homoenxertos criopreservados Crio) implantados em carneiros jovens. MÉTODOS: Foram cinco animais pulmonares e outros cinco, criopreservados. Os apresentaram seguimento médio de 280 ± 14 dias. O diâmetro valvar foi medido por ecocardiografia, a qual realizada no 30º pós-operatório antes do explante. As valvas foram também avaliadas macroscopicamente. A avaliação...
Extracellular vesicles (EVs) are particles released from different cell types and represent key components of paracrine secretion. Accumulating evidence supports the beneficial effects EVs for tissue regeneration. In this study, discarded human heart tissues were used to isolate heart-derived extracellular (hH-EVs). We nanoparticle tracking analysis (NTA) transmission electron microscopy (TEM) physically characterize hH-EVs mass spectrometry (MS) profile protein content in these particles....
Review our long-term results with the Ross operation in middle-aged patients.Between 1995 and 2016, 129 consecutive patients (106 males); mean age (47.2 ± 5.2 years) underwent a operation. Right ventricular outflow tract (RVOT) reconstruction was performed cryopreserved (n = 45) or decellularized allografts 84). Mean follow-up 8.4 5.3 years (0.1 20.5 years). We analyzed early late mortality, as well valve related events need for reoperations.Early mortality 1.6% survival 87.6% at 16 years....
The objective was to analyze the decellularization process with SDS in glutaraldehyde-preserved bovine pericardium as an anticalcification method a circulatory sheep model.The valved tubs were implanted pulmonary artery position by 180 days. animals divided two groups of 8 animals: control group--glutaraldehyde-preserved and study group--decellularized 0,1% glutaraldehyde-preserved. After explantation analized x-ray macroscopy, hematoxilin-eosin, alizarin-red Russel-Movatz pentacromic...
The aim of study was to investigate the SDS-based decellularization process as an anticalcification method in glutaraldehyde-preserved bovine pericardium subcutaneous rat model.Pericardium samples with 0.5 cm² area were divide four groups: group GDA: 0.5% (GDA); GDA-GL: GDA + 0.2% glutamic acid (GL); D-GDA: decellularized (D) 0.1% SDS and D-GDA-GL: acid. After this implanted 18 rats position till 90 days. Each animal received groups. explants performed at 45 subjected histology glass slides...
The primary objective was to evaluate performance of low concentration SDS decellularised porcine pulmonary roots in the right ventricular outflow tract juvenile sheep. Secondary objectives were explore cellular population over time. Animals monitored by echocardiography and explanted at 1, 3, 6 ( n = 4) 12 months 8) for gross analysis. Explanted subject histological, immunohistochemical quantitative calcium analysis 4 3 months) determination material properties 4; months). Cryopreserved...
Real-time polymerase chain reaction (qPCR) using 16S rDNA is an alternative to conventional culture-based tests. The aim of this study was compare the culture method with qPCR in a model cardiac tissue contamination. Samples for artificial contamination Escherichia coli and control samples were submitted DNA extraction, which conducted by selective alkaline lysis purification steps. A standard curve constructed determine efficiency analytical sensitivity assay concentrations from 106 102...
Este trabalho se trata de uma Revisão bibliográfica, sobre desprescrição, que é o processo planejado e supervisionado interrupção ou redução dose um tratamento farmacoterapêutico, não está sendo benéfico ao paciente mostrando resposta farmacêutica satisfatória. Os profissionais responsáveis pelo ato desprescrição deve ter amplo conhecimento em farmacologia, como profissional farmacêutico para a avaliação da somente interações medicamentosas. principais grupos visados prática são: pacientes...
Processos licitatórios têm por objetivo a aquisição de bens e produtos onde há emprego verba pública. Medicamentos insumos para saúde são comercializados obedecendo leis resoluções específicas, sendo comercialização restrita empresas especializadas com responsabilidade técnica do farmacêutico. Porém quando adquiridos licitação medicamentos passam ser categorizados como consumo, vigorando sobre estes legislação não mais específica. Tal fato corrobora que sem as devidas qualificações técnicas,...
Efficacy of AlCl3 and ethanol in the prevention calcification fragments porcine aortic wall fixed GDAEficácia do e etanol na prevenção da calcificação de fragmentos parede aórtica porcina fixados em GDA Resumo Objetivo: Avaliar a eficácia cloreto alumínio, isoladamente ou associação com o etanol, resposta inflamatória fixada glutaraldeído (GDA), implantados no tecido subcutâneo ratos jovens.Método: Utilizaram-se 15 linhagem Sprague-Dawley, cujas telas subcutâneas foram porcina, submetidos...